Strong expression of CD133 is associated with increased cholangiocarcinoma progression

Table 1 Relation between clinicopathological features and immunohistochemical staining of CD133 in cholangiocarcinoma specimens

Variable	Total(n = 34)	CD133 expression		P-value
		Weak	Strong
Age
< 60	18	7	11	0.477
> 60	16	4	12
Sex
Male	22	8	14	0.705
Female	12	3	9
Tumor differentiation
Well	17	7	10	0.465
Moderate/poor	17	4	13
Node
Negative	16	9	7	0.0091
Positive	18	2	16
Distant metastasis
Negative	24	10	14	0.113
Positive	10	1	9
Surgical resection margin2
R0	20	10	10	0.0111
R1, R2	14	1	13
Location of tumor
Perihilar	12	3	9	0.705
Intrahepatic	22	8	14
Neurovascular invasion3
Positive	15	8	7	0.210
Negative	9	2	7

¹Statistically significant;

²Surgical resection margin: R0 = negative resection margin, R1 = microscopic positive resection margin and R2 = macroscopic positive resection margin;

³To study the presence of neurovascular invasion, we excluded 10 specimens derived from tissue biopsy.

Table 2 Survival analysis on clinicopathological parameters by Cox’s multivariate model

Variables	P-value	Hazard ratio	95% CI
			Lower limit	Upper limit
Tumor differentiation	0.57	0.77	0.32	1.86
Lymph node metastasis	0.81	1.15	0.36	3.66
Distant metastasis	0.97	1.03	0.22	4.53
Neurovascular invasion	0.33	1.48	0.67	3.26
CD133 expression	0.35	0.53	0.14	2.02
Surgical resection status	0.041	4.09	1.06	15.72

¹Statistically significant; CI: Confidence interval.