Schistosoma japonicum ova maintains epithelial barrier function during experimental colitis

Table 1 Primers for polymerase chain reaction

Primer	Sequence	Product (bp)
ZO-1 sense	5’-AGCCAGTCCATTCTCAGG-3’	151
ZO-1 antisense	5’-TGTACTGTGAGGGCAACG -3’
Occludin sense	5’-TACAGACCCAAGAGCAGC -3’	177
Occludin antisense	5’-GTGGCAATAAACACCATG -3’
NOD2 sense	5’-CCGAGGAGTCGTGATGGTT-3’	150
NOD2 antisense	5’-GTGTCACCCACATGCAGTG-3’
GAPDH sense	5’-GGTGAAGGTCGGTGTGAACG -3’	233
GAPDH antisense	5’-CTCGCTCCTGGAAGATGGTG-3’

GAPDH: Glyceraldehyde phosphate dehydrogenase.

Table 2 Culture of blood, mesenteric lymph nodes, liver and spleen

	No. of mice	MLN	Blood	Liver	Spleen
Control	10	1/10	0/10	0/10	0/10
TNBS+ova-	10	8/10	10/10	9/10	5/10
TNBS+ova+	10	1/10a	3/10a	2/10a	1/10a

^aP < 0.05 compared with the TNBS group. MLN: Mesenteric lymph nodes; TNBS: Trinitrobenzenesulfonic acid.