Multiphoton microscopy: An introduction to gastroenterologists

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Oct 28, 2011 (publication date) through Apr 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 28, 2011; 17(40): 4456-4460
Published online Oct 28, 2011. doi: 10.3748/wjg.v17.i40.4456

Table 1 Comparison of two-photon microscopy with confocal microscopy

	Confocal microscopy	Two-photon microscopy
Excitation wavelength	Short (ultraviolet light)	Long (infra-red light)
Tissue imaging depth	About 50-100 μm	About 400-1000 μm
Spatial resolution	nm (3D-resolution with pinhole aperture )	nm (3D-resolution with inherent optical sectioning)
Photodamage and photobleaching	High	Low

Citation: Cho HJ, Chun HJ, Kim ES, Cho BR. Multiphoton microscopy: An introduction to gastroenterologists. World J Gastroenterol 2011; 17(40): 4456-4460
URL: https://www.wjgnet.com/1007-9327/full/v17/i40/4456.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i40.4456