rAd-p53 enhances the sensitivity of human gastric cancer cells to chemotherapy

doi:10.3748/wjg.v17.i38.4289

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Oct 14, 2011 (publication date) through Apr 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 14, 2011; 17(38): 4289-4297
Published online Oct 14, 2011. doi: 10.3748/wjg.v17.i38.4289

Table 1 p53 expression in gastric cancer cells 48 h after treatment with rAd-p53, oxaliplatin or rAd-p53 plus oxaliplatin

Treatment	Gastric cancer cell line
	rAd-p53 (vp/mL)	OXA (μg/mL)	SGC-7901	BGC-823	HGC-27
OXA	0	3.2	11.83 ± 1.02^e^b	8.67 ± 1.35^e^b	6.36 ± 1.62^e^b
rAd-p53	5 × 10⁶	0	36.65 ± 1.04^c^a	25.13 ± 2.73^c^a	21.26 ± 1.07^c^a
	5 × 10⁷	0	40.32 ± 1.03^c^a	32.45 ± 2.35^c^a	25.35 ± 1.28^c^a
	5 × 10⁸	0	48.86 ± 1.26^c^a	38.25 ± 2.16^c^a	29.67 ± 1.31^c^a
	5 × 10⁹	0	60.38 ± 1.14^c^a	49.37 ± 1.07^c^a	33.25 ± 2.05^c^a
rAd-p53 + OXA	5 × 10⁶	3.2	37.23 ± 1.07^e^c^a	26.54 ± 1.53^e^c^a	22.17 ± 1.13^e^c^a
	5 × 10⁷	3.2	39.83 ± 1.32^e^c^a	34.17 ± 1.26^e^c^a	24.83 ± 1.07^e^c^a
	5 × 10⁸	3.2	49.03 ± 1.26^e^c^a	40.28 ± 1.43^e^c^a	30.45 ± 1.32^e^c^a
	5 × 10⁹	3.2	61.54 ± 1.18^e^c^a	50.37 ± 1.27^e^c^a	35.21 ± 2.1^e^c^a
Control	0	0	12.55 ± 1.15	8.23 ± 1.13	6.15 ± 1.36

^aP < 0.05 vs control,

^bP > 0.05 vs control;

^cP > 0.05, rAd-p53 vs rAd-p53 + oxaliplatin (OXA) with the same dose of rAd-p53;

^eP < 0.05, OXA vs rAd-p53 + OXA with the same dose of OXA.

Table 2 Bax expression in gastric cancer cells 48 h after treatment with rAd-p53, oxaliplatin or rAd-p53 plus oxaliplatin

Treatment	Gastric cancer cell line
	rAd-p53 (vp/mL)	OXA (μg/mL)	SGC-7901	BGC-823	HGC-27
OXA	0	3.2	73.52 ± 0.83^e^a	56.43 ± 0.74^e^a	36.47 ± 1.21^e^a
rAd-p53	5 × 10⁶	0	63.25 ± 1.32^c^a	53.86 ± 1.54^c^a	33.71 ± 1.41^c^a
	5 × 10⁷	0	76.14 ± 0.73^c^a	59.32 ± 1.45^c^a	39.47 ± 1.03^c^a
	5 × 10⁸	0	79.62 ± 1.46^c^a	64.74 ± 1.08^c^a	41.35 ± 1.15^c^a
	5 × 10⁹	0	82.54 ± 1.28^c^a	69.53 ± 1.02^c^a	43.75 ± 1.1^c^a
rAd-p53 + OXA	5 × 10⁶	3.2	78.82 ± 0.88^e^c^a	58.64 ± 1.07^e^c^a	49.15 ± 1.04^e^c^a
	5 × 10⁷	3.2	84.32 ± 1.02^e^c^a	62.74 ± 1.19^e^c^a	52.9 ± 1.31^e^c^a
	5 × 10⁸	3.2	87.41 ± 1.03^e^c^a	67.38 ± 1.14^e^c^a	55.23 ± 1.06^e^c^a
	5 × 10⁹	3.2	89.71 ± 0.36^e^c^a	75.14 ± 1.65^e^c^a	58.67 ± 1.12^e^c^a
Control	0	0	26.32 ± 1.04	19.91 ± 0.87	16.74 ± 1.23

^aP < 0.05 vs control;

^cP < 0.05, rAd-p53 vs rAd-p53 + oxaliplatin (OXA) with the same dose of rAd-p53;

^eP < 0.05, OXA vs rAd-p53 + OXA with the same dose of OXA.

Table 3 Bcl-2 expression in gastric cancer cells 48 h after treatment with rAd-p53, oxaliplatin or rAd-p53 plus oxaliplatin

Treatment	Gastric cancer cell line
	rAd-p53 (vp/mL)	OXA (μg/mL)	SGC-7901	BGC-823	HGC-27
OXA	0	3.2	26.32 ± 1.21^e^a	47.53 ± 1.13^e^a	56.64 ± 1.33^e^a
rAd-p53	5 × 10⁶	0	28.62 ± 1.07^c^a	58.23 ± 1.04^c^a	61.23 ± 1.07^c^a
	5 × 10⁷	0	24.34 ± 1.05^c^a	46.26 ± 1.31^c^a	49.54 ± 1.14^c^a
	5 × 10⁸	0	18.62 ± 1.32^c^a	40.81 ± 1.15^c^a	47.34 ± 1.06^c^a
	5 × 10⁹	0	15.37 ± 1.51^c^a	38.37 ± 1.08^c^a	44.31 ± 1.03^c^a
rAd-p53+ OXA	5 × 10⁶	3.2	21.76 ± 1.16^e^c^a	35.63 ± 1.41^e^c^a	38.18 ± 1.08^e^c^a
	5 × 10⁷	3.2	18.34 ± 1.24^e^c^a	32.37 ± 1.07^e^c^a	35.71 ± 2.02^e^c^a
	5 × 10⁸	3.2	16.22 ± 1.02^e^c^a	29.27 ± 1.13^e^c^a	32.91 ± 1.24^e^c^a
	5 × 10⁹	3.2	13.14 ± 1.07^e^c^a	26.74 ± 1.02^e^c^a	29.84 ± 1.57^e^c^a
Control	0	0	38.97 ± 1.06	73.71 ± 2.02	84.03 ± 1.02

^aP < 0.05 vs control;

^cP < 0.05, rAd-p53 vs rAd-p53 + oxaliplatin (OXA) with the same dose of rAd-p53;

^eP < 0.05, OXA vs rAd-p53 + OXA with the same dose of OXA.

Table 4 Caspase-3 expression in gastric cancer cells 48 h after treatment with rAd-p53, oxaliplatin or rAd-p53 plus oxaliplatin

Treatment	Gastric cancer cell line
	rAd-p53 (vp/mL)	OXA (μg/mL)	SGC-7901	BGC-823	HGC-27
OXA	0	3.2	12.32 ± 0.8^e^a	11.21 ± 1.05^e^a	8.86 ± 1.01^e^a
rAd-p53	5 × 10⁶	0	7.89 ± 1.13^c^a	6.07 ± 0.97^c^a	4.32 ± 1.03^c^a
	5 × 10⁷	0	10.03 ± 1.03^c^a	8.38 ± 1.04^c^a	6.03 ± 0.99^c^a
	5 × 10⁸	0	12.34 ± 1.05^c^a	10.52 ± 0.89^c^a	8.31 ± 1.02^c^a
	5 × 10⁹	0	15.04 ± 1.03^c^a	11.34 ± 0.55^c^a	10.12 ± 1.01^c^a
rAd-p53 + OXA	5 × 10⁶	3.2	22.05 ± 1.01^e^c^a	15.67 ± 1.03^e^c^a	13.48 ± 1.01^e^c^a
	5 × 10⁷	3.2	25.13 ± 1.06^e^c^a	18.83 ± 1.02^e^c^a	15.32 ± 1.07^e^c^a
	5 × 10⁸	3.2	27.24 ± 1.73^e^c^a	21.07 ± 1.01^e^c^a	18.93 ± 1.06^e^c^a
	5 × 10⁹	3.2	35.67 ± 1.03^e^c^a	26.16 ± 1.05^e^c^a	22.34 ± 1.13^e^c^a
Control	0	0	1.32 ± 1.02	1.29 ± 0.97	1.27 ± 0.68

^aP < 0.05 vs control;

^cP < 0.05, rAd-p53 vs rAd-p53 + oxaliplatin (OXA) with the same dose of rAd-p53;

^eP < 0.05, OXA vs rAd-p53 + OXA with the same dose of OXA.

Table 5 Apoptotic rate in gastric cancer cells 48 h after treatment with rAd-p53, oxaliplatin or rAd-p53 plus oxaliplatin

Treatment	Gastric cancer cell line
	rAd-p53 (vp/mL)	OXA (μg/mL)	SGC-7901	BGC-823	HGC-27
OXA	0	3.2	33.52 ± 1.6^e^a	23.28 ± 1.35^e^a	18.72 ± 1.61^e^a
rAd-p53	5 × 10⁶	0	7.89 ± 1.13^c^a	6.51 ± 0.97^c^a	4.07 ± 0.83^c^a
	5 × 10⁷	0	12.47 ± 1.43^c^a	8.78 ± 1.34^c^a	6.43 ± 0.79^c^a
	5 × 10⁸	0	21.84 ± 1.05^c^a	14.24 ± 0.89^c^a	11.72 ± 1.12^c^a
	5 × 10⁹	0	36.73 ± 1.03^c^a	28.64 ± 1.75^c^a	21.82 ± 1.81^c^a
rAd-p53 + OXA	5 × 10⁶	3.2	42.38 ± 1.51^e^c^a	35.72 ± 1.13^e^c^a	28.84 ± 1.21^e^c^a
	5 × 10⁷	3.2	54.84 ± 1.26^e^c^a	48.63 ± 1.62^e^c^a	34.51 ± 1.47^e^c^a
	5 × 10⁸	3.2	58.41 ± 1.13^e^c^a	51.71 ± 1.41^e^c^a	38.5 ± 1.16^e^c^a
	5 × 10⁹	3.2	63.91 ± 1.23^e^c^a	55.73 ± 1.35^e^c^a	42.92 ± 1.33^e^c^a
Control	0	0	4.67 ± 1.32	1.74 ± 0.67	1.15 ± 0.58

^aP < 0.05 vs control;

^cP < 0.05, rAd-p53 vs rAd-p53 + oxaliplatin (OXA) with the same dose of rAd-p53;

^eP < 0.05, OXA vs rAd-p53 + OXA at the same dose of OXA.

Citation: Chen GX, Zheng LH, Liu SY, He XH. rAd-p53 enhances the sensitivity of human gastric cancer cells to chemotherapy. World J Gastroenterol 2011; 17(38): 4289-4297
URL: https://www.wjgnet.com/1007-9327/full/v17/i38/4289.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i38.4289