Dickkopf3 overexpression inhibits pancreatic cancer cell growth in vitro

Table 1 Oligonucleotide primers used in the study

	Sequence (5’ to 3’)	TA (°C)	Cycles
PCR
Dkk3 (full-length)	Forward: CCCAAGCTTATGCAGCGGCTTGGGGC	53	35
	Reverse: CGCGGATCCCTAAATCTCTTCCCCTCCCAGCAGT
Real-time RT-PCR
Dkk3	Forward: ACAGCCACAGCCTGGTGTA	60	40
	Reverse: CCTCCATGAAGCTGCCAAC
β-catenin	Forward: AAAATGGCAGTGCGTTTAG	60	40
	Reverse: TTTGAAGGCAGTCTGTCGTA
GAPDH	Forward: GCACCGTCAAGGCTGAGAAC	60	40
	Reverse: GCCTTCTCCATGGTGGTGAA
RT-PCR
Dkk3	Forward: AAGGCAGAAGGAGCCACGAGTGC	61	35
	Reverse: GGCCATTTTGGTGCAGTGACCCCA
β-actin	Forward: AAATCGTGCGTGACATTAA	52	35
	Reverse: CTCGTCATACTCCTGCTTG
MSP
Dkk3 unmethylated	Forward: TTAGGGGTGGGTGGTGGGGT[32]	59	34
	Reverse: CTACATCTCCACTCTACACCCA[32]
Dkk3 methylated	Forward: GGGCGGGCGGCGGGGC[32]	59	34
	Reverse: ACATCTCCGCTCTACGCCCG[32]

T_A: Annealing temperature; Real-time RT-PCR: Real-time reverse transcription polymerase chain reaction; Dkk3: Dickkopf3; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MSP: Methylation-specific polymerase chain reaction.