RNAi knockdown of PIK3CA preferentially inhibits invasion of mutant PIK3CA cells

Table 1 PIK3CA mutation in gastric cancer cell lines

Cell line	Nucleotide substitution	Amino acid change	Exon	Domain
HGC-27	G1633A	E545K	9	Helical
MKN-45	A3140G	H1047R	20	Kinase

Detection of PIK3CA mutation in exons 9 and 20 was screened by PCR followed by sequencing in 5 gastric cancer cell lines (HGC-27, SGC-7901, BGC-823, MGC-803 and MKN-45). Only two cell lines carried mutations of PIK3CA. HGC-27 cells contained the G1633A (E545K) mutation in exon 9 and MKN-45 contained the A3140G (H1047R) mutation in exon 20.