Switching-on of serotonergic calcium signaling in activated hepatic stellate cells

Table 1 Primers for reverse transcription-polymerase chain reaction

Name	Sequence	Accession code	Position	Product (bp)
5-HT1A
(+)	5'-TCAGCTACCAAGTGATCACC-3'	NM_012585.1	98-117	211
(-)	5'-GTCCACTTGTTGAGCACCTG-3'		308-289
5-HT1B
(+)	5'-TACACGGTCTACTCCACGGT-3'	NM_022225.1	610-629	258
(-)	5'-TCGCACTTTGACTTGGTTCAC-3'		867-847
5-HT2A
(+)	5'-GTGTCCATGTTAACCATCCT-3'	NM_017254	446-465	376
(-)	5'-GTAGGTGATCACCATGATGG-3'		821-802
5-HT2B
(+)	5'-CATGCATCTCTGTGCCATTTC-3'	NM_017250	652-672	352
(-)	5'-TGTTAGGCGTTGAGGTGGC-3'		1003-985
5-HT3A
(+)	5'-TCCTCAACGTGGATGAGAAG-3'	NM_024394.1	553-572	352
(-)	5'-ATGTTGATGTCCTGGATGGT-3'		904-885
5-HT3B
(+)	5'-AAGCCCATCCAGGTGGTCTC-3'	NM_022189.1	459-478	428
(-)	5'-GACATGTTGACCCTGAAGAC-3'		886-867
5-HT4
(+)	5'-TCATGGTGCTGGCCTATTAC-3'	NM_012853.1	640-659	377
(-)	5'-CTCATCATCACAGCAGAGGA-3'		1016-997
5-HT5A
(+)	5'-GAACAGGAGGAAGGAAGAGA-3'	NM_013148	1535-1554	109
(-)	5'-TAAGTCTCCTTGGTGTGAGG-3'		1643-1624
5-HT5B
(+)	5'-TTCACCGTACTCGTGGTAAC-3'	L10073.1	453-472	132
(-)	5'-GGTCGAGGCTACCAAGTTAT-3'		584-565
5-HT6
(+)	5'-CCTGAGAGTGTGCTGAATTG-3'	NM_024365.1	1716-1735	129
(-)	5'-AGCCACACTACACAAGCAAC-3'		1844-1825
5-HT7
(+)	5'-GTGTGTCCACTGTCAAATCC-3'	NM_022938	2072-2091	148
(-)	5'-TCACTCATCTCCAGTTACCG-3'		2219-2200

5-HT: 5-hydroxytryptamine.

Table 2 Primers for quantitative reverse transcription-polymerase chain reaction

Name	Sequence	Accession code	Position	Product (bp)
5-HT2A
(+)	5'-GGGTACCTCCCACCGACAT-3'	NM_	234-252	101
(-)	5'-TTTCCAGCAATGGTGAGAATAATC-3'	17254	334-311
5-HT2B
(+)	5'-CGCCATCCCAGTCCCTATTA-3'	NM_	781-800	101
(-)	5'-AGAGCATGAAACTGCCAAAGC-3'	17250	881-861
IP3R 1
(+)	5'-GCAGAAGCAGATTGGCTATG-3'	NM_	2072-2091	261
(-)	5'-GTCTCAATCAGGATGTCAGC-3'	1007235	2332-2313
IP3R 2
(+)	5'-CAAGAAGTTCAGAGACTGCC-3'	NM_	396-415	295
(-)	5'-ACGCATGGCATTCTTCTCCA-3'	31046.3	690-671
IP3R 3
(+)	5'-GATGTGGTGTTGCTGCAGAA-3'	NM_	390-409	137
(-)	5'-TTGTTGCTCTTCATGTGCAG-3'	13138.1	526-507
RyR 1
(+)	5'-CTGAATGTCTGCTCTCCAAG-3'	AC	35577-35596	112
(-)	5'-GAAGGGCAGAGAGACAAGAT-3'	165142.3	35688-35669
RyR 2
(+)	5'-ATGTAGGCTTCTTCCAGAGC-3'	XR_	11405-11414	136
(-)	5'-TGCAGTACCTTCTCTCCTGA-3'	8338.1	11540-11521
RyR 3
(+)	5'-TACCTTGCCTGGTACACAAC-3'	XM_001080527.1	13957-13976	123
(-)	5'-AGTCACAGATGACAGGATCG-3'		14079-14060
SERCA 1
(+)	5'-CCAAGGAGCCTCTTATCAGT-3'	NM_017254	2516-2535	111
(-)	5'-CCTCTGCATACAAGAACCAC-3'		2626-2607
SERCA 2
(+)	5'-AGTTCATCCGCTACCTCATC-3'	M 23114	2297-2316	119
(-)	5'-CACCAGATTGACCCAGAGTA-3'		2415-2396
SERCA 3
(+)	5'-CTCATGCAGAAGGAGTTCAC-3'	NM_172812	1563-1582	140
(-)	5'-CGCTCAATTACACTCTCAGG-3'		1702-1683
Calreticulin
(+)	5'-AGAAGACTGGGATGAACGAG-3'	NM_	683-701	109
(-)	5'-GTCCTCAGGCTTCTTAGCAT-3'	22399.1	791-772
Calsequestrin-2
(+)	5'-CAGATGGCTATGAGTTCCTG-3'	NM_	988-1007	118
(-)	5'-CAGTAAGCAACAAGCAGAGG-3'	17131.2	1105-1086
Calnexin
(+)	5'-GTGTTTGCTACTGGTCCTTG-3'	NM_	21-40	146
(-)	5'-ATGGAGGAGTGCTGGTATCT-3'	172008.1	166-147
TGF-β type 1 R
(+)	5'-ACCAGCTATTGCCCATAGAG-3'	L 26110	1011-1030	106
(-)	5'-GGCAGAATCATGTCTCACAG-3'		1116-1097
α-SMA
(+)	5'-GCAGAGCAAGAGGGATCCT-3'	X 06801	222-242	73
(-)	5'-CATGTCGTCCCAGTTGGTGAT-3'		294-274
Cav1.2 (α1c)
(+)	5'-GACCCGTAGGAGCACGTTTG-3'	NM_012517	2327-2346	71
(-)	5'-CCTCCCCGGTCAGGATCT-3'		2397-2380

5-HT: 5-hydroxytryptamine; SERCA: Sarcoplasmic/endoplasmic reticulum Ca²⁺ ATPase; α-SMA: α-smooth muscle actin; RyR: Ryanodine receptor; TGF: Transforming growth factor.