β-catenin accumulation in nuclei of hepatocellular carcinoma cells up-regulates glutathione-s-transferase M3 mRNA

Table 1 Primer sets used in mRNA differential display analysis

Anchor primers	Arbitrary primers
T12MG (10 μmol/L)	AP-10 (2 μmol/L), 5’-TAGCAAGTGC-3’
T12MA (10 μmol/L)	AP-11 (2 μmol/L), 5’-CAGACCGTTC-3’
T12MT (10 μmol/L)	AP-12 (2 μmol/L), 5’-TGCTGACCTG-3’
T12MC (10 μmol/L)	AP-14 (2 μmol/L), 5’-AATGGGCTGA-3’

M represents a degenerated mixture of dA, dG and dC. The 16 different primer sets used for PCR amplification were randomly combined from the four arbitrary primers and the four anchor primers.

Table 2 Total glutathione-s-transferase activity in B6C3F1 mice hepatocellular carcinoma cells (T) and matched nontumorous (N) liver tissue samples

Samples	Total GST activity (nmol/mg per min)		Values of GST activity ratio (T-N)/N
	T	N
(+)	3122 ± 189	2447 ± 180	0.353 ± 0.117a
	(n = 13)	(n = 13)
(-)	2644 ± 199	2523 ± 205	0.071 ± 0.064
	(n = 11)	(n = 11)

[(T-N)/N] indicates the glutathione-s-transferase (GST) activity values for the samples with and without β-catenin accumulation in hepatocellular carcinoma (HCC) cell nuclei. “(+) or (-)” denotes the samples from B6C3F1 mice with or without β-catenin accumulation in nuclei of HCC cells.

^aP < 0.001 vs negative β-catenin staining group.