MiRNA profile in esophageal squamous cell carcinoma: Downregulation of miR-143 and miR-145

Table 1 Clinical characteristics of esophageal squamous cell carcinoma used for quantitative reverse transcription-polymerase chain reaction analysis

Clinical parameter	n
Gender
Male	62
Female	24
Age (yr)
< 55	35
≥ 55	51
Diameter
< 5 cm	57
≥ 5 cm	29
LNM
N0	53
N1	33
Invasion
T1	4
T2	18
T3	64
Histological type
Ulcerative	49
Medullary	24
Fungating	10
Others	3
Differentiation
I	23
II	51
III	12
TNM stage
I	3
IIa	49
IIb	4
III	30

Table 2 Associations between expression levels of miR-143 and miR-145 and clinicopathological features

	miR-143		miR-145
	median ± SD	P value	median ± SD	P value
Gender
Male	1.43 ± 2.99	0.805	0.82 ± 1.61	0.718
Female	1.27 ± 1.72		0.95 ± 1.32
Age (yr)
< 55	1.67 ± 3.54	0.418	1.15 ± 1.99	0.138
≥ 55	1.19 ± 1.92		0.65 ± 1.08
Diameter (cm)
< 5	1.47 ± 2.02	0.701	0.93 ± 1.31	0.497
≥ 5	1.23 ± 3.71		0.69 ± 1.89
LNM
N0	1.47 ± 3.17	0.717	0.88 ± 1.71	0.815
N1	1.25 ± 1.70		0.80 ± 1.16
Tumor stage
T1	6.31 ± 9.33	0.000b	3.33 ± 4.70	0.001b
T2	0.80 ± 1.32		0.34 ± 0.30
T3	1.25 ± 1.87		0.84 ± 1.26
Histological type
Ulcerative	1.45 ± 3.28	0.996	0.79 ± 1.76	0.955
Medullary	1.29 ± 1.72		0.90 ± 1.23
Fungating	1.32 ± 1.70		0.90 ± 1.13
Others	1.48 ± 1.18		1.27 ± 0.98
Differentiation
I	1.60 ± 2.11	0.907	0.87 ± 1.31	0.925
II	1.30 ± 3.12		0.81 ± 1.70
III	1.38 ± 1.60		1.00 ± 1.15
TNM stage
I	0.76 ± 1.00	0.971	0.94 ± 1.39	0.998
IIa	1.41 ± 2.18		0.83 ± 1.31
IIb	1.06 ± 1.47		0.95 ± 1.48
III	1.46 ± 3.60		0.87 ± 1.90

^bP < 0.01.

Table 3 miR-143 and miR-145 predicted targets related to cell mobility

	Predicted targets	Functions
miR-143	LASP1 (LIM and SH3 protein 1)	Functions as an actin-binding protein and possibly in cytoskeletal organization
	RICTOR (RPTOR independent companion of MTOR, complex 2)	Functions upstream of Rho GTPases to regulate the actin cytoskeleton
	ARHGAP26 (Rho GTPase activating protein 26)	Regulates the organization of the actin-cytoskeleton
	EPB41 [erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)]	Together with spectrin and actin, constitute the red cell membrane cytoskeletal network.
	MYO3A (myosin IIIA)	Belongs to the myosin superfamily, an actin-dependent motor protein
	MARCKS (myristoylated alanine-rich protein kinase C substrate)	An actin filament crosslinking protein involved in cell motility
	SVIL (supervillin)	Tightly associated with both actin filaments and plasma membranes
miR-145	ARF6 (ADP-ribosylation factor 6)	Regulates vesicular trafficking, remodeling of membrane lipids, and signaling pathways that lead to actin remodeling.
	ABLIM2 (actin binding LIM protein family, member 2)	Bound strongly to F-actin, localized to actin stress fibers
	ADD3 [adducin 3 (γ)]	Involved in assembly of the spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues
	CAPZB (c apping protein (actin filament) muscle Z-line, β)	Regulates growth of the actin filament by capping the barbed end of growing actin filaments.
	ELMO1 (engulfment and cell motility 1)	Mediates cytoskeletal rearrangements during phagocytosis of apoptotic cells and cell motility
	PHACTR2 (phosphatase and actin regulator 2)	Coprecipitated with both PP1 and actin
	TMOD1 (tropomodulin 1)	Inhibits depolymerization and elongation of the pointed end of actin filaments