MicroRNA155 is induced in activated CD4+ T cells of TNBS-induced colitis in mice

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Feb 21, 2010 (publication date) through Nov 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 21, 2010; 16(7): 854-861
Published online Feb 21, 2010. doi: 10.3748/wjg.v16.i7.854

Table 1 Primers used for RT or PCR of mRNA or miRNA

Gene name		Primer sequences (5’-3’)
IL-1β	Sense	GCAACTGTTCCTGAACTCAACT
IL-1β	Antisense	ATCTTTTGGGGTCCGTCAACT
IL-6	Sense	CCACTTCACAAGTCGGAGGCTTA
IL-6	Antisense	GCAAGTGCATCATCGTTGTTCATAC
IFN-γ	Sense	TCAAGTGGCATAGATGTGGAAGAA
IFN-γ	Antisense	TGGCTCTGCAGGATTTTCATG
TNF	Sense	CCACCACGCTCTTCTGTCTAC
TNF	Antisense	TGGGCTACAGGCTTGTCACT
β-actin	Sense	CTAGGCACCAGGGTGTGAT
β-actin	Antisense	TGCCAGATCTTCTCCATGTC
miRNA155	Stem-loop primer	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCCT
	Sense	GCCCGCTTAATGCTAATTGTGAT
	Antisense	GTGCAGGGTCCGAGGT
U6	Sense	CTCGCTTCGGCAGCACA
U6	Antisense	AACGCTTCACGAATTTGCGT

RT: Reverse transcription; PCR: Polymerase chain reaction.

Citation: Chen DF, Gong BD, Xie Q, Ben QW, Liu J, Yuan YZ. MicroRNA155 is induced in activated CD4⁺ T cells of TNBS-induced colitis in mice. World J Gastroenterol 2010; 16(7): 854-861
URL: https://www.wjgnet.com/1007-9327/full/v16/i7/854.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i7.854