Analgesic effects of JCM-16021 on neonatal maternal separation-induced visceral pain in rats

Table 1 Composition of JCM-16021 and related quality analysis results

Composition and samples	Atractylone1	Gallic acid1	Corilagin2	Paeoniflorin3	Magnolol4	Honokiol5	Tetrahydropalmatine6	Quercitrin7	Heavy metal & pesticide residues
Fructus Terminaliae Chebulae (Terminalia chebula Retz.) 9%		+	2.18%						Pass
Radix Paeoniae Lactiflorae (Paeonia lactiflora Pall.) 14%				0.45%					Pass
Cortex Magnoliae Officinalis (Magnolia officinalis Rehd. et Wils.) 9%					1.76%	0.89%			Pass
Rhizoma Corydalis Yanhusuo (Corydalis yanhusuo W. T. Wang) 14%							0.10%		Pass
Herba Polygoni Chinensis (Polygonum chinense L) 18%								0.04%	Pass
Rhizoma Atractylodis Macrocephalae (Atractylodes macrocephala Koidz) 18%	+								Pass
Semen coicis Lachryma-jobi [Coix lacryma-jobi L. var. ma-yuan (Roman.) Stapf] 18%									Pass
Final product	-	+	2.43%	0.70%	ND	ND	0.01%	ND	Pass

This table presents the composition of formula and its ratio of each component in whole formula.

¹TLC test, + means detected, - means not detected;

²Y = 4309.9x + 11.158, R² = 1, 0.155-7.75 μg;

³Y = 1141.8x + 54.916, R² = 0.9999, 0.322-18.343 μg;

⁴Y = 7822.7x + 1167.9, R² = 0.9994, 0.375-3.75 μg;

⁵Y=7947.5x + 1465.9, R² = 0.9982, 0.34-3.4 μg;

⁶Y = 5782.6x - 11.063, R² = 1, 0.028-1.4 μg;

⁷Y=2612.4x + 1.6674, R² = 1, 0.0228-1.14 μg; ND means that the chemical marker was detected but not determined due to peak area being too small.