Redefining the properties of an osmotic agent in an intestinal-specific preservation solution

Table 1 Characteristics of high-purity dextrans

Group	Mp	Mw	Mn	PDI	IV
D1	62 900	72 700	50 700	1.43	0.263
D2	238 000	275 900	204 600	1.35	0.463
D3	490 000	534 000	371 000	1.43	0.633
D4	1 050 000	1 185 000	705 000	1.67	0.862
D5	2 000 000	2 400 000	1 600 000	1.50	0.803

Mp: Peak average molecular weight; Mw: Weight average molecular weight; Mn: Number average molecular weight; PDI: Polydispersity index (PDI = Mw/Mn); IV: Intrinsic viscosity (dL/g).

Table 2 Intestinal morphology following cold ischemic storage

Time (h)	Group	Grade	Median	Significance	Event
2	D1	0, 2, 6, 7	4		Denuded villi
	D2	3, 4, 6, 6	5		Loss of villi
	D3	1, 2, 2, 3	2	D3, D4, D5 vs D1, D2, P = 0.06	Moderate clefting
	D4	1, 2, 3, 5	2.5		Moderate-extensive clefting
	D5	1, 2, 3, 4	2.5		Moderate-extensive clefting
12	D1	0, 6, 7, 8	6.5		Injury to crypts and submucosa
	D2	2, 6, 7, 7	6.5		Injury to crypts and submucosa
	D3	3, 7, 8, 8	7.5		Injury to crypts, submucosa and muscularis
	D4	3, 6, 8, 8	7		Injury to crypts and submucosa
	D5	0, 4, 4, 5	4	D5 vs D1-D4, P < 0.05	Clefting and denuded villi

A modified Park’s classification for intestinal injury was used to assess mucosal morphology as follows: Grade 0: Normal mucosa; Grade 1: Subepithelial space at villus tip; Grade 2: Moderate subepithelial space; Grade 3: Epithelial lifting along villus sides; Grade 4: Denuded villi; Grade 5: Loss of villus tissue; Grade 6: Crypt layer injury; Grade 7: Transmucosal injury; Grade 8: Transmural injury.