Original Article
Copyright ©2010 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 28, 2010; 16(44): 5565-5581
Published online Nov 28, 2010. doi: 10.3748/wjg.v16.i44.5565
Table 1 Relevant parameters in serum (mean ± SE)
GroupAmylase (IU/L)Lipase (IU/L)TNF-α (pg/mL)LDH (IU/L)
Stress (-)
Veh402 ± 2312 ± 2BD299 ± 21
Cer569 ± 3144 ± 3BD358 ± 23
Anti-TNF-α + Cer572 ± 4342 ± 5BD327 ± 27
Stress (+)
Veh475 ± 2121 ± 312 ± 2a612 ± 41
Cer3997 ± 226a1793 ± 118a42 ± 4a3410 ± 218a
Anti-TNF-α + Cer1895 ± 103c714 ± 45c16 ± 2c1534 ± 121c
Table 2 Histopathological score in pancreatic tissue (mean ± SE)
GroupEdemaHemorrhageLeukocyte infiltrateNecrosis
Stress (-)
Veh0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
Cer0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
Anti-TNF-α + Cer0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
Stress (+)
Veh0.49 ± 0.060.00 ± 0.000.39 ± 0.050.00 ± 0.00
Cer1.28 ± 0.11a3.11 ± 0.17a2.19 ± 0.10a3.12 ± 0.15a
Anti-TNF-α + Cer2.19 ± 0.17c1.21 ± 0.17c1.32 ± 0.10c1.37 ± 0.19c
Table 3 Inflammatory parameters in pancreatic tissue (mean ± SE)
GroupTNF-α (ng/mg DNA)HIF-1α (ng/mg DNA)Active trypsin (nmol/mg DNA)NF-κB (p65) (μg/mg DNA)NF-κB (p50) (μg/mg DNA)MPO (U/mg DNA)LPO (nmol/mg DNA)MDA (μmol/mg DNA)
Stress (-)
VehBDBD0.11 ± 0.0243 ± 50.48 ± 0.040.00 ± 0.00BD0.71 ± 0.03
CerBDBD0.23 ± 0.0446 ± 60.51 ± 0.050.00 ± 0.00BD0.89 ± 0.05
Anti-TNF-α + CerBDBD0.22 ± 0.0544 ± 30.50 ± 0.030.00 ± 0.00BD0.84 ± 0.05
Stress (+)
Veh1.6 ± 0.2a0.9 ± 0.1a0.37 ± 0.0672 ± 60.84 ± 0.071.41 ± 0.0727 ± 22.05 ± 0.11
Cer5.4 ± 0.3a3.6 ± 0.3a4.15 ± 0.32a389 ± 25a5.54 ± 0.35a15.90 ± 0.66a316 ± 20a22.16 ± 0.69a
Anti-TNF-α + Cer2.0 ± 0.2c1.5 ± 0.3c1.79 ± 0.21c221 ± 18c2.65 ± 0.24c7.14 ± 0.38c171 ± 14c12.38 ± 0.47c
Table 4 Inflammatory parameters in blood (mean ± SE)
GroupLeukocytes (ng/mL)CRP (mg/dL)HSP-72 (pg/mL)IL-6 (pg/mL)IL-10 (pg/mL)MIP-1α (pg/mL)
Stress (-)
Veh 5900 ± 2400.0 ± 0.0558 ± 27BDBD7 ± 1
Cer 6111 ± 2480.0 ± 0.0866 ± 32BDBD9 ± 1
Anti-TNF-α + Cer 6005 ± 2440.0 ± 0.0817 ± 49BDBD8 ± 1
Stress (+)
Veh 7139 ± 2501.5 ± 0.2a899 ± 6366 ± 6a75 ± 5a54 ± 4a
Cer21 222 ± 976a24.9 ± 1.3a2648 ± 159a1115 ± 54a1404 ± 148a318 ± 23a
Anti-TNF-α + Cer12 733 ± 586c12.4 ± 0.8c1805 ± 112c417 ± 38c596 ± 68c127 ± 11b
Table 5 General biochemical assays in blood (mean ± SE)
GroupGlucose (mg/dL)Urea (mg/dL)Creatinine (mg/dL)Calcium (mg/dL)Proteins (g/dL)AST (IU/L)ALT (IU/L)Hematocrit (%)
Stress (-)
Veh139 ± 1339 ± 40.48 ± 0.0313.02 ± 0.578.51 ± 0.39192 ± 2188 ± 740 ± 5
Cer153 ± 1455 ± 40.65 ± 0.0911.08 ± 0.487.46 ± 0.28298 ± 32155 ± 1442 ± 4
Anti-TNF-α + Cer148 ± 1252 ± 50.67 ± 0.0810.91 ± 0.707.51 ± 0.43293 ± 20149 ± 941 ± 5
Stress (+)
Veh118 ± 11a38 ± 50.50 ± 0.0511.72 ± 0.618.48 ± 0.22201 ± 2695 ± 936 ± 5a
Cer294 ± 26a105 ± 9a1.94 ± 0.50a9.03 ± 0.33a6.02 ± 0.19a432 ± 35a219 ± 19a41 ± 3
Anti-TNF-α + Cer189 ± 16c73 ± 8c1.14 ± 0.24c9.63 ± 0.586.76 ± 0.35c356 ± 29c187 ± 19c39 ± 4
Table 6 Pancreatic caspases (mean ± SE)
GroupCASP-2CASP-3CASP-8CASP-9
Stress (-)
Veh34 ± 625 ± 245 ± 540 ± 7
Cer56 ± 1263 ± 987 ± 1182 ± 8
Anti-TNF-α + Cer50 ± 739 ± 572 ± 768 ± 9
Stress (+)
Veh124 ± 15118 ± 10189 ± 11158 ± 16
Cer995 ± 88a838 ± 74a1123 ± 103a1079 ± 70a
Anti-TNF-α + Cer696 ± 47c544 ± 58c887 ± 65c679 ± 48c
Table 7 Energy metabolism in pancreatic tissue (mean ± SE)
GroupATP (μmol/mg protein)ATP loss (%)ADP/ATP ratio
Stress (-)
Veh9.1 ± 0.40.0 ± 4.40.32 ± 0.04
Cer6.8 ± 0.525.3 ± 5.50.38 ± 0.05
Anti-TNF-α + Cer6.9 ± 0.624.2 ± 6.60.36 ± 0.04
Stress (+)
Veh7.3 ± 0.519.8 ± 5.50.40 ± 0.06
Cer2.6 ± 0.4a71.4 ± 4.4a0.94 ± 0.11a
Anti-TNF-α + Cer5.3 ± 0.3c41.8 ± 3.3c0.63 ± 0.07c
Table 8 Histopathological score in lung tissue (mean ± SE)
GroupEdemaHemorrhageLeukocyte infiltrate
Stress (-)
Veh0.00 ± 0.000.00 ± 0.000.00 ± 0.00
Cer0.00 ± 0.000.00 ± 0.000.00 ± 0.00
Anti-TNF-α + Cer0.00 ± 0.000.00 ± 0.000.00 ± 0.00
Stress (+)
Veh0.36 ± 0.040.00 ± 0.000.30 ± 0.03
Cer1.51 ± 0.11a2.73 ± 0.58a1.63 ± 0.54a
Anti-TNF-α + Cer1.80 ± 0.21c1.21 ± 0.08c0.73 ± 0.05c
Table 9 Inflammatory parameters in lung tissue (mean ± SE)
GroupTNF-α (ng/mg DNA)MPO (U/mg DNA)NF-κB (p65) (μg/mg DNA)NF-κB (p50) (μg/mg DNA)
Stress (-)
VehBD0.00 ± 0.0023 ± 20.27 ± 0.02
CerBD0.00 ± 0.0024 ± 20.30 ± 0.02
Anti-TNF-α + CerBD0.00 ± 0.0024 ± 30.29 ± 0.03
Stress (+)
Veh1.3 ± 0.1a0.89 ± 0.1243 ± 50.51 ± 0.03
Cer4.2 ± 0.3a12.10 ± 0.56a251 ± 19a2.92 ± 0.28a
Anti-TNF-α + Cer1.8 ± 0.2c5.04 ± 0.29c107 ± 9c1.13 ± 0.11c
Table 10 Lung caspases (mean ± SE)
GroupCASP-2CASP-3CASP-8CASP-9
Stress (-)
Veh18 ± 320 ± 221 ± 522 ± 4
Cer27 ± 329 ± 432 ± 529 ± 3
Anti-TNF-α + Cer26 ± 224 ± 324 ± 422 ± 3
Stress (+)
Veh97 ± 11103 ± 999 ± 8104 ± 12
Cer853 ± 81a912 ± 101a824 ± 77a917 ± 85a
Anti-TNF-α + Cer597 ± 48c592 ± 55c560 ± 45c568 ± 47c
Table 11 Energy metabolism in lung tissue (mean ± SE)
GroupATP (μmol/mg protein)ATP loss (%)ADP/ATP ratio
Stress (-)
Veh10.3 ± 0.50.0 ± 4.90.20 ± 0.03
Cer10.0 ± 0.32.9 ± 2.90.22 ± 0.02
Anti-TNF-α + Cer10.1 ± 0.41.9 ± 3.80.21 ± 0.03
Stress (+)
Veh7.9 ± 0.725.5 ± 6.80.32 ± 0.05
Cer3.6 ± 0.4a66.0 ± 3.8a0.72 ± 0.10a
Anti-TNF-α + Cer6.7 ± 0.6c36.8 ± 5.7c0.49 ± 0.06c
Table 12 Histopathological score in stomach tissue (mean ± SE)
GroupEdemaHemorrhageLeukocyte infiltrateNecrosis
Stress (-)
Veh0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
Cer0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
Anti-TNF-α + Cer0.00 ± 0.000.00 ± 0.000.00 ± 0.000.00 ± 0.00
Stress (+)
Veh3.25 ± 0.14b1.55 ± 0.08a1.26 ± 0.19a0.95 ± 0.06a
Cer1.17 ± 0.08a3.23 ± 0.29a2.77 ± 0.16a3.48 ± 0.32a
Anti-TNF-α + Cer1.96 ± 0.17c2.23 ± 0.15c1.63 ± 0.05c1.57 ± 0.12c
Table 13 Stomach caspases (mean ± SE)
GroupCASP-2CASP-3CASP-8CASP-9
Stress (-)
Veh37 ± 432 ± 348 ± 539 ± 5
Cer40 ± 532 ± 551 ± 440 ± 3
Anti-TNF-α + Cer33 ± 430 ± 347 ± 439 ± 4
Stress (+)
Veh1358 ± 132a1515 ± 154a1479 ± 136a1425 ± 126a
Cer798 ± 87a824 ± 95a868 ± 70a832 ± 87a
Anti-TNF-α + Cer1089 ± 98c1203 ± 107c1108 ± 89c1144 ± 102c
Table 14 Energy metabolism in stomach tissue (mean ± SE)
GroupATP (μmol/mg protein)ATP loss (%)ADP/ATP ratio
Stress (-)
Veh13.4 ± 0.90.0 ± 6.70.16 ± 0.02
Cer12.9 ± 0.73.7 ± 5.20.18 ± 0.03
Anti-TNF-α + Cer13.0 ± 0.83.0 ± 6.00.17 ± 0.02
Stress (+)
Veh5.5 ± 0.4a58.9 ± 3.0a0.67 ± 0.07a
Cer2.3 ± 0.4a82.8 ± 3.0a1.14 ± 0.16a
Anti-TNF-α + Cer6.2 ± 0.6c53.7 ± 4.5c0.58 ± 0.06c