Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer

doi:10.3748/wjg.v16.i37.4738

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 16, Issue 37

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (4816)

All Articles published online

The chart showing PDF series, HTML series, Tables (1-7) series.

Item

Count

PDF

441

HTML

4256

Tables (1-7)

119

Sum=4816

Oct 7, 2010 (publication date) through Apr 29, 2024

Times Cited of This Article

Times Cited (38)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Article

World J Gastroenterol. Oct 7, 2010; 16(37): 4738-4746
Published online Oct 7, 2010. doi: 10.3748/wjg.v16.i37.4738

Table 1 Primers used for genotyping

SNP		Primers	Concentration (μmol/L)
rs4998557	Forward	5’-CGGTGTGGTGTGGATGTTGTG-3’	1.0
	Reverse	5’-GCCCCCAGGAGAGGACTGATT-3’	1.0
	SF	5’-TTTTTTTTTTTTTCCATTACCTGAATGGCTATACTGCTT-3’	0.8
rs4880	Forward	5’-CGGGCTGTGCTTTCTCGTCTT-3’	2.0
	Reverse	5’-GCCAACGCCTCCTGGTACTTC-3’	2.0
	SR	5’-TTTTGGAGCCCAGATACCCCAAA-3’	1.6

SNP: Single nucleotide polymorphism; SF: Single-base extension forward; SR: Single-base extension reverse.

Table 2 Demographic characteristics and risk factors for gastric cancer and malignant potential of gastric precancerous lesion

Variable	HC (%)	GPL (%)	GC (%)	¹P	GC vs GPL	GC vs HC
Variable	n = 147	n = 145	n = 140	¹P	²OR (95% CI)/P	²OR (95% CI)/P
Age (yr), mean ± SD	54.8 ± 9.6	55.8 ± 11.4	56.5 ± 10.3	0.370
Sex
Female	50 (34.0)	35 (24.1)	37 (26.4)		1	1
Male	97 (66.0)	110 (75.9)	103 (73.6)	0.146	1.13 (0.66-1.93)/0.656	0.70 (0.42-1.16)/0.163
Smoking³
No	114 (77.6)	104 (71.7)	102 (72.9)		1	1
Yes	33 (22.4)	41 (28.3)	38 (27.1)	0.484	0.95 (0.56-1.59)/0.831	1.29 (0.75-2.20)/0.358
Alcohol⁴
No	100 (68.0)	69 (47.6)	55 (39.3)		1	1
Yes	47 (32.0)	76 (52.4)	85 (60.7)	< 0.001	1.40 (0.88-2.25)/0.158	3.29 (2.03-5.34)/< 0.001
FH⁵
No	135 (91.8)	120 (82.8)	99 (73.8)		1	1
Yes	12 (8.2)	25 (17.2)	41 (26.2)	< 0.001	1.99 (1.13-3.49)/0.017	4.66 (2.33-9.32)/< 0.001
H. pylori
Negative	76 (51.7)	39 (26.9)	26 (18.6)		1	1
Type I	65 (44.2)	98 (67.6)	108 (77.1)	< 0.001	1.65 (0.94-2.91)/0.082	4.86 (2.83-8.35)/< 0.001
Type II	6 (4.1)	8 (5.5)	6 (4.3)	0.132	1.13 (0.35-3.62)/0.843	2.92 (0.87-9.86)/0.084

¹Gastric cancer (GC) vs gastric precancerous lesion (GPL) vs healthy control (HC), P values were calculated by One-Way ANOVA analysis, χ² test; ²OR (odds ratio), 95% CI (95% confidence interval), P values were calculated by logistic regression;

³Smoking history, at least one cigarette per day for 6 mo or longer;

⁴Alcohol drinking history, at least twice a week for 6 mo or longer and at least 100 g each time;

⁵Family history of gastric cancer, first-degree relatives with gastric cancer (parents, brothers or sisters). FH: Family history of gastric cancer.

Table 3 Distribution of SOD1 G7958A and SOD2 Val16Ala polymorphisms

Variable	HC (%)	GPL (%)	GC (%)	¹P	GC vs GPL	GC vs HC
Variable	n = 147	n = 145	n = 140	¹P	²OR (95% CI)/P	²OR(95% CI)/P
SOD1
GG	78 (53.1)	47 (32.4)	39 (27.9)		1.00	1.00
AG	57 (38.8)	72 (49.7)	72 (51.4)		1.16 (0.67-2.02)/0.593	2.60 (1.54-4.39)/< 0.001
AA	12 (8.2)	26 (17.9)	29 (20.7)	< 0.001³	1.32 (0.66-2.62)/0.429	4.94 (2.26-10.80)/< 0.001
A/-	69 (46.9)	98 (67.6)	101 (72.1)	< 0.001⁴	1.21 (0.72-2.03)/0.483	3.01 (1.83-4.95)/< 0.001
SOD2
Val/Val	119 (81.0)	112 (77.2)	85 (60.7)		1.00	1.00
Ala/Val	27 (18.4)	31 (21.4)	48 (34.3)		2.04 (1.19-3.49)/0.009	2.57 (1.48-4.48)/0.001
Ala/Ala	1 (0.7)	2 (1.4)	7 (5.0)	0.001⁵	4.50 (0.91-22.29)/0.065	10.68 (1.26-90.79)/0.030
Ala/-	28 (19.0)	33 (22.8)	55 (39.3)	< 0.001⁶	2.19 (1.30-3.68)/0.003	2.85 (1.66-4.89)/< 0.001

¹P values were calculated by χ² test; ²Adjusted for age, sex, OR, 95% CI, P values were calculated by logistic regression;

³GG vs AG vs AA;

⁴GG vs A/-;

⁵Val/Val vs Ala/Val vs Ala/Ala;

⁶Val/Val vs Ala/-. A/-: AA + AG; Ala/-: Ala/Val + Ala/Ala. HC: Healthy control; GPL: Gastric precancerous lesion; GC: Gastric cancer.

Table 4 Interaction of family history and SOD2 Val16Ala for malignant potential of gastric precancerous lesion

Family history	SOD2	GPL (%)	GC (%)	¹OR(95% CI)	β	γ = β_eg/β_e
Family history	SOD2	n = 145	n = 140	¹OR(95% CI)	β	γ = β_eg/β_e
Negative	Val/Val	89 (61.4)	58 (41.4)	1.00
Negative	Ala/-	31 (21.4)	41 (29.3)	2.22 (1.24-3.97)	0.80
Positive	Val/Val	24 (16.6)	27 (19.3)	1.99 (1.03-3.84)	0.69
Positive	Ala/-	1 (0.7)	14 (10.0)	7.71 (2.10-28.22)	2.04	2.96

A/-: AA + AG; Ala/-: Ala/Val + Ala/Ala. β: Regressive coefficient; γ: Interaction coefficients; β_eg: Regressive coefficient when genetic and environmental factors coexist; β_e: Regressive coefficient when environmental factors exist alone. ¹Adjusted for age, sex, OR, 95% CI, β were calculated by logistic regression. GPL: Gastric precancerous lesion; GC: Gastric cancer.

Table 5 Interaction of SOD1 G7958A and environmental factors for gastric cancer development

Factors	SOD1	HC (%)	GC (%)	¹OR (95% CI)	β	γ = β_eg/β_e
Factors	SOD1	n = 147	n = 140	¹OR (95% CI)	β	γ = β_eg/β_e
Alcohol
No	GG	58 (39.5)	10 (7.1)	1.00
No	A/-	42 (28.6)	45 (32.1)	6.00 (2.70-13.33)	1.79
Yes	GG	21 (14.3)	29 (20.7)	10.30 (3.94-26.91)	2.33
Yes	A/-	26 (17.7)	56 (40.0)	16.50 (6.67-40.86)	2.80	1.20
Family history
Negative	GG	73 (49.7)	25 (17.9)	1.00
Negative	A/-	62 (42.2)	74 (52.9)	3.58 (2.02-6.37)	1.28
Positive	GG	6 (4.1)	14 (10.0)	7.70 (2.63-22.51)	2.04
Positive	A/-	6 (4.1)	27 (19.3)	15.56 (5.57-43.50)	2.75	1.35
Type I H. pylori
Negative	GG	55 (37.4)	10 (7.1)	1.00
Negative	A/-	27 (18.4)	22 (15.7)	4.48 (1.84-10.91)	1.50
Positive	GG	24 (16.3)	29 (20.7)	6.41 (2.69-15.27)	1.86
Positive	A/-	41 (27.9)	79 (56.4)	10.71 (4.92-23.33)	2.37	1.27

A/-: AA + AG; Ala/-: Ala/Val + Ala/Ala.

¹Adjusted for age, sex, OR, 95% CI, β were calculated by logistic regression. HC: Healthy control; GC: Gastric cancer.

Table 6 Interaction of SOD2 Val16Ala and environmental factors for gastric cancer development

Factors	SOD2	HC (%)	GC (%)	¹OR (95% CI)	β	γ = β_eg/β_e
Factors	SOD2	n = 147	n = 140	¹OR (95% CI)	β	γ = β_eg/β_e
Alcohol
No	Val/Val	89 (60.5)	33 (23.6)	1.00
No	Ala/-	11 (7.5)	21 (15.0)	4.89 (2.11-11.36)	1.59
Yes	Val/Val	34 (23.1)	52 (37.1)	5.26 (2.62-10.55)	1.66
Yes	Ala/-	13 (8.8)	34 (24.3)	9.46 (4.08-21.94)	2.25	1.36
Family history
Negative	Val/Val	114 (77.6)	58 (41.4)	1.00
Negative	Ala/-	21 (14.3)	41 (29.3)	3.90 (2.09-7.27)	1.36
Positive	Val/Val	9 (6.1)	27 (19.3)	6.34 (2.75-14.59)	1.85
Positive	Ala/-	3 (2.0)	14 (10.0)	12.86 (3.36-49.25)	2.55	1.38
Type I H. pylori
Negative	Val/Val	75 (51.0)	23 (16.4)	1.00
Negative	Ala/-	7 (4.8)	9 (6.4)	4.90 (1.59-15.08)	1.59
Positive	Val/Val	48 (32.7)	61 (43.6)	4.24 (2.30-7.09)	1.44
Positive	Ala/-	17 (11.6)	47 (33.6)	9.07 (4.36-18.86)	2.21	1.53

A/-: AA + AG; Ala/-: Ala/Val + Ala/Ala. ¹Adjusted for age, sex, OR, 95% CI, β were calculated by logistic regression. HC: Healthy control; GC: Gastric cancer.

Table 7 Interaction of SOD1 G7958A and SOD2 Val16Ala for gastric cancer development

Genotype		HC (%)	GC (%)	¹OR (95% CI)	P
SOD1	SOD2	n = 147	n = 140	¹OR (95% CI)	P
GG	Val/Val	72 (49.0)	31 (22.1)	1.00
A/-	Val/Val	50 (34.0)	54 (38.6)	2.54 (1.43-4.52)	0.002
GG	Ala/-	7 (4.8)	8 (5.7)	2.69 (0.89-8.14)	0.079
A/-	Ala/-	18 (12.2)	47 (33.6)	6.43 (3.20-12.91)	< 0.001

A/-: AA + AG; Ala/-: Ala/Val + Ala/Ala. ¹Adjusted for age, sex, OR, 95% CI and P values were calculated by logistic regression. HC: Healthy control; GC: Gastric cancer.

Citation: Yi JF, Li YM, Liu T, He WT, Li X, Zhou WC, Kang SL, Zeng XT, Zhang JQ. Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer. World J Gastroenterol 2010; 16(37): 4738-4746
URL: https://www.wjgnet.com/1007-9327/full/v16/i37/4738.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i37.4738