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©2010 Baishideng Publishing Group Co.
World J Gastroenterol. Oct 7, 2010; 16(37): 4738-4746
Published online Oct 7, 2010. doi: 10.3748/wjg.v16.i37.4738
Published online Oct 7, 2010. doi: 10.3748/wjg.v16.i37.4738
Table 1 Primers used for genotyping
SNP | Primers | Concentration (μmol/L) | |
rs4998557 | Forward | 5’-CGGTGTGGTGTGGATGTTGTG-3’ | 1.0 |
Reverse | 5’-GCCCCCAGGAGAGGACTGATT-3’ | 1.0 | |
SF | 5’-TTTTTTTTTTTTTCCATTACCTGAATGGCTATACTGCTT-3’ | 0.8 | |
rs4880 | Forward | 5’-CGGGCTGTGCTTTCTCGTCTT-3’ | 2.0 |
Reverse | 5’-GCCAACGCCTCCTGGTACTTC-3’ | 2.0 | |
SR | 5’-TTTTGGAGCCCAGATACCCCAAA-3’ | 1.6 |
Table 2 Demographic characteristics and risk factors for gastric cancer and malignant potential of gastric precancerous lesion
Variable | HC (%) | GPL (%) | GC (%) | 1P | GC vs GPL | GC vs HC |
n = 147 | n = 145 | n = 140 | 2OR (95% CI)/P | 2OR (95% CI)/P | ||
Age (yr), mean ± SD | 54.8 ± 9.6 | 55.8 ± 11.4 | 56.5 ± 10.3 | 0.370 | ||
Sex | ||||||
Female | 50 (34.0) | 35 (24.1) | 37 (26.4) | 1 | 1 | |
Male | 97 (66.0) | 110 (75.9) | 103 (73.6) | 0.146 | 1.13 (0.66-1.93)/0.656 | 0.70 (0.42-1.16)/0.163 |
Smoking3 | ||||||
No | 114 (77.6) | 104 (71.7) | 102 (72.9) | 1 | 1 | |
Yes | 33 (22.4) | 41 (28.3) | 38 (27.1) | 0.484 | 0.95 (0.56-1.59)/0.831 | 1.29 (0.75-2.20)/0.358 |
Alcohol4 | ||||||
No | 100 (68.0) | 69 (47.6) | 55 (39.3) | 1 | 1 | |
Yes | 47 (32.0) | 76 (52.4) | 85 (60.7) | < 0.001 | 1.40 (0.88-2.25)/0.158 | 3.29 (2.03-5.34)/< 0.001 |
FH5 | ||||||
No | 135 (91.8) | 120 (82.8) | 99 (73.8) | 1 | 1 | |
Yes | 12 (8.2) | 25 (17.2) | 41 (26.2) | < 0.001 | 1.99 (1.13-3.49)/0.017 | 4.66 (2.33-9.32)/< 0.001 |
H. pylori | ||||||
Negative | 76 (51.7) | 39 (26.9) | 26 (18.6) | 1 | 1 | |
Type I | 65 (44.2) | 98 (67.6) | 108 (77.1) | < 0.001 | 1.65 (0.94-2.91)/0.082 | 4.86 (2.83-8.35)/< 0.001 |
Type II | 6 (4.1) | 8 (5.5) | 6 (4.3) | 0.132 | 1.13 (0.35-3.62)/0.843 | 2.92 (0.87-9.86)/0.084 |
Table 3 Distribution of SOD1 G7958A and SOD2 Val16Ala polymorphisms
Variable | HC (%) | GPL (%) | GC (%) | 1P | GC vs GPL | GC vs HC |
n = 147 | n = 145 | n = 140 | 2OR (95% CI)/P | 2OR(95% CI)/P | ||
SOD1 | ||||||
GG | 78 (53.1) | 47 (32.4) | 39 (27.9) | 1.00 | 1.00 | |
AG | 57 (38.8) | 72 (49.7) | 72 (51.4) | 1.16 (0.67-2.02)/0.593 | 2.60 (1.54-4.39)/< 0.001 | |
AA | 12 (8.2) | 26 (17.9) | 29 (20.7) | < 0.0013 | 1.32 (0.66-2.62)/0.429 | 4.94 (2.26-10.80)/< 0.001 |
A/- | 69 (46.9) | 98 (67.6) | 101 (72.1) | < 0.0014 | 1.21 (0.72-2.03)/0.483 | 3.01 (1.83-4.95)/< 0.001 |
SOD2 | ||||||
Val/Val | 119 (81.0) | 112 (77.2) | 85 (60.7) | 1.00 | 1.00 | |
Ala/Val | 27 (18.4) | 31 (21.4) | 48 (34.3) | 2.04 (1.19-3.49)/0.009 | 2.57 (1.48-4.48)/0.001 | |
Ala/Ala | 1 (0.7) | 2 (1.4) | 7 (5.0) | 0.0015 | 4.50 (0.91-22.29)/0.065 | 10.68 (1.26-90.79)/0.030 |
Ala/- | 28 (19.0) | 33 (22.8) | 55 (39.3) | < 0.0016 | 2.19 (1.30-3.68)/0.003 | 2.85 (1.66-4.89)/< 0.001 |
Table 4 Interaction of family history and SOD2 Val16Ala for malignant potential of gastric precancerous lesion
Family history | SOD2 | GPL (%) | GC (%) | 1OR(95% CI) | β | γ = βeg/βe |
n = 145 | n = 140 | |||||
Negative | Val/Val | 89 (61.4) | 58 (41.4) | 1.00 | ||
Negative | Ala/- | 31 (21.4) | 41 (29.3) | 2.22 (1.24-3.97) | 0.80 | |
Positive | Val/Val | 24 (16.6) | 27 (19.3) | 1.99 (1.03-3.84) | 0.69 | |
Positive | Ala/- | 1 (0.7) | 14 (10.0) | 7.71 (2.10-28.22) | 2.04 | 2.96 |
Table 5 Interaction of SOD1 G7958A and environmental factors for gastric cancer development
Factors | SOD1 | HC (%) | GC (%) | 1OR (95% CI) | β | γ = βeg/βe |
n = 147 | n = 140 | |||||
Alcohol | ||||||
No | GG | 58 (39.5) | 10 (7.1) | 1.00 | ||
No | A/- | 42 (28.6) | 45 (32.1) | 6.00 (2.70-13.33) | 1.79 | |
Yes | GG | 21 (14.3) | 29 (20.7) | 10.30 (3.94-26.91) | 2.33 | |
Yes | A/- | 26 (17.7) | 56 (40.0) | 16.50 (6.67-40.86) | 2.80 | 1.20 |
Family history | ||||||
Negative | GG | 73 (49.7) | 25 (17.9) | 1.00 | ||
Negative | A/- | 62 (42.2) | 74 (52.9) | 3.58 (2.02-6.37) | 1.28 | |
Positive | GG | 6 (4.1) | 14 (10.0) | 7.70 (2.63-22.51) | 2.04 | |
Positive | A/- | 6 (4.1) | 27 (19.3) | 15.56 (5.57-43.50) | 2.75 | 1.35 |
Type I H. pylori | ||||||
Negative | GG | 55 (37.4) | 10 (7.1) | 1.00 | ||
Negative | A/- | 27 (18.4) | 22 (15.7) | 4.48 (1.84-10.91) | 1.50 | |
Positive | GG | 24 (16.3) | 29 (20.7) | 6.41 (2.69-15.27) | 1.86 | |
Positive | A/- | 41 (27.9) | 79 (56.4) | 10.71 (4.92-23.33) | 2.37 | 1.27 |
Table 6 Interaction of SOD2 Val16Ala and environmental factors for gastric cancer development
Factors | SOD2 | HC (%) | GC (%) | 1OR (95% CI) | β | γ = βeg/βe |
n = 147 | n = 140 | |||||
Alcohol | ||||||
No | Val/Val | 89 (60.5) | 33 (23.6) | 1.00 | ||
No | Ala/- | 11 (7.5) | 21 (15.0) | 4.89 (2.11-11.36) | 1.59 | |
Yes | Val/Val | 34 (23.1) | 52 (37.1) | 5.26 (2.62-10.55) | 1.66 | |
Yes | Ala/- | 13 (8.8) | 34 (24.3) | 9.46 (4.08-21.94) | 2.25 | 1.36 |
Family history | ||||||
Negative | Val/Val | 114 (77.6) | 58 (41.4) | 1.00 | ||
Negative | Ala/- | 21 (14.3) | 41 (29.3) | 3.90 (2.09-7.27) | 1.36 | |
Positive | Val/Val | 9 (6.1) | 27 (19.3) | 6.34 (2.75-14.59) | 1.85 | |
Positive | Ala/- | 3 (2.0) | 14 (10.0) | 12.86 (3.36-49.25) | 2.55 | 1.38 |
Type I H. pylori | ||||||
Negative | Val/Val | 75 (51.0) | 23 (16.4) | 1.00 | ||
Negative | Ala/- | 7 (4.8) | 9 (6.4) | 4.90 (1.59-15.08) | 1.59 | |
Positive | Val/Val | 48 (32.7) | 61 (43.6) | 4.24 (2.30-7.09) | 1.44 | |
Positive | Ala/- | 17 (11.6) | 47 (33.6) | 9.07 (4.36-18.86) | 2.21 | 1.53 |
Table 7 Interaction of SOD1 G7958A and SOD2 Val16Ala for gastric cancer development
Genotype | HC (%) | GC (%) | 1OR (95% CI) | P | |
SOD1 | SOD2 | n = 147 | n = 140 | ||
GG | Val/Val | 72 (49.0) | 31 (22.1) | 1.00 | |
A/- | Val/Val | 50 (34.0) | 54 (38.6) | 2.54 (1.43-4.52) | 0.002 |
GG | Ala/- | 7 (4.8) | 8 (5.7) | 2.69 (0.89-8.14) | 0.079 |
A/- | Ala/- | 18 (12.2) | 47 (33.6) | 6.43 (3.20-12.91) | < 0.001 |
- Citation: Yi JF, Li YM, Liu T, He WT, Li X, Zhou WC, Kang SL, Zeng XT, Zhang JQ. Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer. World J Gastroenterol 2010; 16(37): 4738-4746
- URL: https://www.wjgnet.com/1007-9327/full/v16/i37/4738.htm
- DOI: https://dx.doi.org/10.3748/wjg.v16.i37.4738