Up-regulation of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in sentinel lymph nodes of colorectal cancer

Table 1 Identification of differentially expressed proteins in normal lymph nodes and sentinel lymph node micrometastasis of colorectal cancer

Spot ID	International proteinindex accession No.	Protein	Score	Sequence coverage (%)	Mr/pI
Up
185	IPI00307162	VCL Isoform 2 of Vinculin	98	11	124 292/5.50
482	IPI00168698	PDZD8 PDZ domain-containing protein 8	198	15	129 681/5.78
5151	IPI00027834	HNRNPL heterogeneous nuclear ribonucleoprotein L isoform a	119	17	64 720/8.46
501	IPI00216952	LMNA Isoform C of Lamin-A/C	190	41	65 153/6.40
530	IPI00289499	ATIC Bifunctional purine biosynthesis protein PURH	252	46	65 089/6.27
741	IPI00021187	RUVBL1 Isoform 1 of RuvB-like 1	133	39	50 538/6.02
1071	IPI00027444	SERPINB1 Leukocyte elastase inhibitor	94	38	42 829/5.90
12661	IPI00007752	TUBB2C Tubulin β-2C chain	134	30	50 255/4.79
1411	IPI00103433	TCP11 Isoform 1 of T-complex protein 11 homolog	108	24	56 675/5.08
14491	IPI00218918	ANXA1 Annexin A1	196	50	38 918/6.57
1649	IPI00554767	CLIC1 18 kDa protein	96	52	17 927/4.71
16501	IPI00746388	EZR Ezrin	133	21	69 353/5.88
1967	IPI00878282	ALB 23 kDa protein	94	36	23 414/5.93
2102	IPI00220766	GLO1 Lactoylglutathione lyase	107	48	20 992/5.12
2410	IPI00216691	PFN1 Profilin-1	62	40	15 216/8.44
Down
391	IPI00021405	LMNA Isoform A of Lamin-A/C	204	42	74 380/6.57
509	IPI00022463	TF Serotransferrin precursor	65	19	79 280/6.81
701	IPI00878282	ALB 23 kDa protein	127	52	23 414/5.93
719	IPI00553177	SERPINA1 Isoform 1 of α-1-antitrypsin precursor	247	52	46 878/5.37
994	IPI00027223	IDH1 Isocitrate dehydrogenase (NADP) cytoplasmic	150	50	46 915/6.53
1042	IPI00021926	PSMC6 26S protease regulatory subunit S10B	85	25	44 430/7.10
1204	IPI00298497	FGB Fibrinogen β chain precursor	204	46	56 577/8.54
1433	IPI00455315	ANXA2 Annexin A2	130	41	38 808/7.57
1442	IPI00295889	SRP19 Signal recognition particle 19 kDa protein	129	38	16 374/9.87
1550	IPI00872780	ANXA4 Annexin A4	217	60	36 088/5.84
1572	IPI00745868	ANXA3 Uncharacterized protein ANXA3 (Fragment)	121	41	36 623/5.53
1715	IPI00394878	C1QTNF1 C1q and tumor necrosis factor related protein 1	273	68	22 841/8.40
1856	IPI00003766	ETHE1 ETHE1 protein, mitochondrial precursor	124	45	28 368/6.35
1863	IPI00465028	TPI1 Isoform 1 of Triosephosphate isomerase	292	80	31 057/5.65
1890	IPI00025512	HSPB1 Heat shock protein β-1	86	48	22 826/5.98
1919	IPI00853525	APOA1 Apolipoprotein A1	191	64	28 005/5.80
1954	IPI00220766	GLO1 Lactoylglutathione lyase	107	48	20 992/5.12
1961	IPI00219622	PSMA2 Proteasome subunit α type-2	275	70	25 996/ 6.92
1995	IPI00003815	ARHGDIA Rho GDP-dissociation inhibitor 1	91	43	23 250/5.02
2104	IPI00014832	PDK2 [Pyruvate dehydrogenase kinase isozyme 2	152	19	51 389/7.67
2237	IPI00096066	SUCLG2 Succinyl-CoA ligase [GDP-forming]	184	23	46 824/6.15
2245	IPI00384679	RNF170 20 kDa protein	184	54	20 773/6.40
2349	IPI00796366	MYL6B 16 kDa protein	97	51	16 451/4.56
2540	IPI00295844	RP11-429E11.3 Novel protein	153	65	15 089/5.49
2564	IPI00796636	HBB Hemoglobin (Fragment)	98	64	11 554/5.90

¹Over-expressed proteins in sentinel lymph nodes and their function-related metastasis of cancer cells.

Table 2 Expression of hnRNP A1, Ezrin, tubulin β-2C, and Annexin A1 in normal lymph nodes and sentinel lymph node micrometastasis

Group	Case	hnRNP A1			Ezrin			Tubulin β-2C			Annexin A1
		N	P	Rate (%)	N	P	Rate (%)	N	P	Rate (%)	N	P	Rate (%)
NLN	62	28	34	54.8	57	5	8.1	50	12	19.3	53	9	14.5
SLNMM	126	38	88	69.8	16	110	87.3	32	94	74.6	58	68	53.9
χ2 vlue		4.11			109.84			51.57			26.75
P value		0.05 > P > 0.01			< 0.01			< 0.01			< 0.01

NLN: Normal lymph nodes; SLNMM: Sentinel lymph node micrometastasis; N: Negative; P: Positive.