Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients

Table 1 Primers and conditions used in nested reverse transcripts-polymerase chain reaction

Primers	Primers from 5' to 3'	PCR conditions denaturation annealing extension
MAGE-1 outer primers	Forward primer: 5'-CGGCCGAAGGAACCTGACCCAG-3'	94°C for 45 s
	Reverse primer: 5'-GCTGGAACCCTCACTGGGTTGCC-3'	65°C for 45 s
		72°C for 45 s
MAGE-1 inner primers	Forward primer: 5'-ACAGAGGAGCACCAAGGAGAAG-3'	94°C for 45 s
	Reverse primer: 5'-AGTTGATGGTAGTGGGAAAGGC-3'	65°C for 45 s
		72°C for 45 s
SSX-1 outer primers	Forward primer: 5'-CTAAAGCATCAGAGAAGAGAAGC-3'	94°C for 60 s
	Reverse primer: 5'-AGATCTCTTATTAATCTTCTCAGAAA-3'	57°C for 60 s
		72°C for 60 s
SSX-1 inner primers	Forward primer: 5'-TCAGAGAAGAGAAGCAAGGCCTTT-3'	94°C for 45 s
	Reverse primer: 5'-TTCTCAGAAATATTTGCTTTTCC-3'	56°C for 45 s
		72°C for 45 s
CTp11 outer primers	Forward primer: 5'-CTGCCCCAGACATTGAAGAA-3'	94°C for 45 s
	Reverse primer: 5'-TCCATGAATTCCTCCTCCTC-3'	57°C for 60 s
		72°C for 90 s
CTp11 inner primers	Forward primer: 5'-TGTGAATCCAACGAGGTG-3'	94°C for 45 s
	Reverse primer: 5'-TTGATTCTGTTCTCTCGGGC-3'	60°C for 45 s
		72°C for 45 s

PCR: Polymerase chain reaction; MAGE-1: Melanoma antigen-1; SSX-1: Synovial sarcoma X breakpoint-1; CTp11: Cancer-testis-associated protein of 11 kDa.