Expression of protein S100A4 is a predictor of recurrence in colorectal cancer

Table 1 Relationship between expression of protein S100A4, E-cadherin, p53 and clinicopathologic parameters n (%)

Parameters	Expression of protein S100A4			Expression of E-cadherin			Expression of p53
	Negative	Positive	P value	Reduced	Preserved	P value	Negative	Positive	P value
	(n = 82)	(n = 45)		(n = 48)	(n = 79)		(n = 56)	(n = 71)
Gender			0.465			0.787			0.364
Male	51 (62.2)	25 (55.6)		28 (58.3)	48 (60.8)		36 (64.3)	40 (56.3)
Female	31 (37.8)	20 (44.4)		20 (41.7)	31 (39.2)		20 (35.7)	31 (43.7)
Age (yr)			0.797			0.963			0.344
mean ± SD	58.4 ± 11.1	60.9 ± 11.2		59.3 ± 13.1	59.4 ± 10.0		58.3 ± 9.6	60.2 ± 12.3
Tumor location			0.352			0.120			0.528
Colon	38 (46.3)	17 (37.8)		25 (52.1)	30 (38.0)		26 (46.4)	29 (40.8)
Rectum	44 (53.7)	28 (62.2)		23 (47.9)	49 (62.0)		30 (53.6)	42 (59.2)
Differentiation			0.5001			0.0011			0.695
Differentiated	75 (91.5)	42 (93.3)		39 (81.3)	78 (98.7)		51 (91.1)	66 (93.0)
Undifferentiated	7 (8.5)	3 (6.7)		9 (18.8)	1 (1.3)		5 (8.9)	5 (7.0)
Depth of tumor			0.319			0.615			0.154
T1-2	25 (30.5)	10 (22.2)		12 (25.0)	23 (29.1)		19 (33.9)	16 (22.5)
T3-4	57 (69.5)	35 (77.8)		36 (75.0)	56 (70.9)		37 (66.1)	55 (77.5)
Lymph node metastasis			0.282			0.338			0.667
Absent	50 (61.0)	23 (51.1)		25 (52.1)	48 (60.8)		31 (55.4)	42 (59.2)
Present	32 (39.0)	22 (48.9)		23 (47.9)	31 (39.2)		25 (44.6)	29 (40.8)
Distant metastasis			0.0531			0.3651			0.6541
Absent	81 (98.8)	41 (91.1)		45 (93.8)	77 (97.5)		53 (94.6)	69 (97.2)
Present	1 (1.2)	4 (8.9)		3 (6.3)	2 (2.5)		3 (5.4)	2 (2.8)
pTNM stage			0.175			0.403			0.857
I	17 (20.7)	7 (15.6)		6 (12.5)	18 (22.8)		11 (19.6)	13 (18.3)
II	33 (40.2)	16 (35.6)		19 (39.6)	30 (38.0)		20 (35.7)	29 (40.8)
III	31 (37.8)	18 (40.0)		20 (41.7)	29 (36.7)		22 (39.3)	27 (38.0)
IV	1 (1.2)	4 (8.9)		3 (6.3)	2 (2.5)		3 (5.4)	2 (2.8)
Recurrence2			0.004			0.269			0.845
Absent	67 (82.7)	24 (58.5)		31 (68.9)	60 (77.9)		40 (75.5)	51 (73.9)
Present	14 (17.3)	17 (41.5)		14 (31.1)	17 (22.1)		13 (24.5)	18 (26.1)

¹Calculated by Fisher’s exact test; ²Stage IV patients were excluded.

Table 2 Correlations of protein S100A4 and E-cadherin

S100A4	E-cadherin	n (%)
Co-expression pattern
Negative	Negative	55 (39.9)
Negative	Positive	34 (24.6)
Positive	Negative	32 (23.2)
Positive	Positive	17 (12.3)

Kendall’s Tau-b correlation coefficient = -0.035, P = 0.681.

Table 3 Correlations of protein S100A4 and p53

S100A4	p53	n (%)
Co-expression pattern
Negative	Negative	35 (25.4)
Negative	Positive	54 (39.1)
Positive	Negative	26 (18.8)
Positive	Positive	23 (16.7)

Kendall’s Tau-b correlation coefficient = -0.132, P = 0.120.

Table 4 Univariate overall survival analysis for seven clinicopathologic parameters

Parameters	n	5-yr overall survival rate (%)	P value
Gender			0.817
Male	76	80.9
Female	51	78.0
Differentiation			0.001
Differentiated	117	82.3
Undifferentiated	10	50.0
Depth of tumor			0.001
T1-2	35	97.1
T3-4	92	72.9
Lymph node metastasis			0.001
Absent	73	88.5
Present	54	68.0
S100A4 expression			0.044
Negative	82	86.1
Positive	45	68.3
E-cadherin			0.105
Preserved	79	83.1
Reduced	48	74.3
p53			0.218
Negative	56	83.6
Positive	71	76.6

Table 5 Cox regression analysis on those parameters shown to significantly influence overall survival in a univariate analysis

Parameters	Hazard ratio	95% CI	P value
Lymph node metastasis	2.283	1.014-5.141	0.046
Depth of tumor	10.374	1.391-77.352	0.022
Differentiation	2.748	1.059-7.133	0.038
S100A4 expression	1.985	0.953-4.134	0.067