Effects of blueberry on hepatic fibrosis and transcription factor Nrf2 in rats

doi:10.3748/wjg.v16.i21.2657

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Jun 7, 2010 (publication date) through Jan 30, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. Jun 7, 2010; 16(21): 2657-2663
Published online Jun 7, 2010. doi: 10.3748/wjg.v16.i21.2657

Table 1 Primer sequences for real-time RT-PCR analyses

Genes	GenBank
Genes	Number	Forward	Reverse
β-actin	V01217	TCCTCCTGAGCGCAAGTACTCT	GCTCAGTAACAGTCCGCCTAGAA
Nrf2	NM_057152	CCATGCCTTCTTCCACGAA	AGGGCCCATGGATTTCAGTT
Nqo1	NM_017000	CCAATCCTCCACCCACTTGT	GTCCCTCAGCCATTGTTTGAG

RT-PCR: Reverse transcriptase-polymerase chain reaction.

Table 2 Liver index, serum HA, ALT, and SOD, MDA, GST of liver homogenate in different groups (mean ± SD)

Group	n	Liver index (relative liver weight)	HA (ng/mL)	ALT (U/L)	SOD (μ/mg)	MDA (nmol/mg)	GST (μ/mg)
A	8	0.03 ± 0.000	351.75 ± 125.16	57.25 ± 6.88	1.76 ± 0.34	0.206 ± 0.052	5.95 ± 1.97
B	8	0.054 ± 0.009^a	828.50 ± 237.83^a	203.25 ± 31.62^a	1.08 ± 0.19^a	0.335 ± 0.056^a	7.30 ± 1.26^a
C	9	0.038 ± 0.008^c	502.33 ± 110.57^c	149.44 ± 16.51^a^c	1.36 ± 0.09^c	0.294 ± 0.026^a^c	7.37 ± 0.87^a
D	8	0.036 ± 0.007^c	524.25 ± 255.42^c	136.88 ± 10.07^a^c	1.42 ± 0.13^c	0.285 ± 0.025^a^c	7.35 ± 0.88^a
E	8	0.036 ± 0.005^c	499.25 ± 198.10^c	127.38 ± 11.03^a^c	1.50 ± 0.15^c	0.284 ± 0.028^a^c	7.81 ± 1.16^a

^aP < 0.05 vs group A;

^cP < 0.05 vs group B. HA: Hyaluronic acid; ALT: Alanine aminotransferase; SOD: Superoxide dismutase; MDA: Malondialdehyde; GST: Glutathione-S-transferase.

Table 3 Degree of hepatic fibrosis in each group of rats

Group	n	Degree of hepatic fibrosis							Mean rank
Group	n	0	1	2	3	4	5	6	Mean rank
A	8	5	3	0	0	0	0	0	4.5
B	8	0	0	0	0	1	6	1	34.25^a
C	9	0	0	3	2	2	2	0	23.00^a^c
D	8	0	0	4	1	1	1	1	22.38^a^c
E	8	0	0	4	1	2	1	0	20.63^a^c

^aP < 0.05 vs group A;

^cP < 0.05 vs group B.

Table 4 Expression of Nrf2 and Nqo1 in each group (mean ± SD)

Group	n	Relative amount of mRNA		Protein expression (%)		Protein level
Group	n	Nrf2	Nqo1	Nrf2	Nqo1	Nrf2	Nqo1
A	8	46.86 ± 6.49	13.38 ± 4.37	57.50 ± 26.14	15.25 ± 3.62	0.21 ± 0.03	0.06 ± 0.03
B	8	254.88 ± 55.96^a	68.38 ± 12.61^a	78.75 ± 15.29^a	42.50 ± 16.26^a	0.30 ± 0.06^a	0.16 ± 0.02^a
C	9	333.44 ± 90.53^a	72.78 ± 15.94^a	85.11 ± 13.65^a	54.44 ± 15.09^a	0.34 ± 0.08^a	0.18 ± 0.03^a
D	8	277.00 ± 67.38^a	70.63 ± 20.17^a	80.00 ± 21.38^a	50.63 ± 18.98^a	0.33 ± 0.09^a	0.17 ± 0.04^a
E	8	345.00 ± 72.60^a	77.88 ± 27.50^a	87.25 ± 14.16^a	58.13 ± 15.80^a	0.35 ± 0.09^a	0.18 ± 0.02^a

^aP < 0.05 vs group A.

Citation: Wang YP, Cheng ML, Zhang BF, Mu M, Wu J. Effects of blueberry on hepatic fibrosis and transcription factor Nrf2 in rats. World J Gastroenterol 2010; 16(21): 2657-2663
URL: https://www.wjgnet.com/1007-9327/full/v16/i21/2657.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i21.2657