NOD2/CARD15, ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn’s disease

Table 1 Demographics and clinical features of patients with CD (mean ± SD) n (%)

	Childhood-onset CD (n = 110)	Adult-onset CD (n = 364)
Sex (male/female)	60/50	178/186
Age of diagnosis (yr)	11.5 ± 4.8	28.99 ± 14.22
Family history in first-degree relative	8 (7.27)	12 (3.29)
Smoking habit
Never		146 (40.11)
Ex-smoker		32 (8.79)
Current		186 (51.10)
Localization of disease
Ileitis	21 (19.09)	121 (33.24)
Colitis	29 (26.36)	38 (10.50)
Ileocolitis	48 (43.64)	187 (51.38)
Upper gastroenteric	12 (10.91)	18 (4.94)
Disease features
Inflammatory	78 (70.91)	223 (61.26)
Stricturing	20 (18.18)	99 (27.20)
Fistulizing	12 (10.91)	42 (11.54)
Extraintestinal manifestations	21 (19.09)	58 (15.93)

CD: Crohn’s disease.

Table 2 Genotype and allele frequencies of NOD2/CARD15 polymorphisms in childhood-onset CD patients, adult-onset CD, and controls n (%)

	Childhood-onset CD (n = 110)	Adult-onset CD (n = 364)	Controls (n = 539)
R702W
Genotype
CC	94 (85.45)	300 (82.40)	482 (89.40)
CT	14 (12.72)	62 (17.00)	55 (10.20)
TT	2 (1.81)	2 (0.55)	2 (0.37)
P value1	NS
P value2	NS
P value3		0.0260
T allele	18 (8.18)	66 (9.10)	59 (5.47)
P value1, OR (95% CI)	NS
P value2, OR (95% CI)	NS
P value3, OR (95% CI)		0.0040, 1.72 (1.19-2.48)
G908R
Genotype
GG	91 (82.73)	295 (81.00)	468 (86.83)
GC	17 (15.45)	65 (18.00)	69 (12.80)
CC	2 (1.81)	4 (1.00)	2 (0.37)
P value1	NS
P value2	NS
P value3		0.0420
C allele	21 (9.54)	73 (10.00)	73 (6.77)
P value1, OR (95% CI)	NS
P value2, OR (95% CI)	NS
P value3, OR (95% CI)		0.0140, 1.53 (1.09-2.15)
3020insC
Genotype
-	78 (70.90)	301 (82.69)	503 (93.32)
insC/-	28 (25.45)	57 (15.66)	35 (6.49)
insC/insC	4 (3.64)	6 (1.65)	1 (0.18)
P value1	< 0.0001
P value2	0.0200
P value3		< 0.0001
insC allele	36 (16.36)	69 (9.47)	37 (3.43)
P value1, OR (95% CI)	< 0.0001, 5.5 (3.39-8.94)
P value2, OR (95% CI)	0.0067, 1.87 (1.21-2.88)
P value3, OR (95% CI)		< 0.0001, 2.95 (2.04-4.44)

¹Childhood-onset vs controls;

²Childhood-onset vs adult onset;

³Adult onset vs controls. NS: Not significant.

Table 3 Genotype and allele frequencies of ATG16L1 polymorphism rs2241880 and IL23R polymorphism rs11209026 in childhood-onset CD patients, adult-onset CD, and controls n (%)

	Childhood-onset CD (n = 110)	Adult-onset CD (n = 364)	Controls (n = 539)
rs2241880
Genotype
AA	17 (15.45)	46 (12.64)	104 (19.30)
AG	45 (40.91)	177 (48.63)	274 (50.83)
GG	48 (43.64)	141 (38.74)	161 (29.90)
P value1	0.0190
P value2	NS
P value3		0.0040
G allele	141 (64.09)	459 (63.05)	596 (55.29)
P value1, OR (95% CI)	0.0170, 1.44 (1.07-1.95)
P value2, OR (95% CI)	NS
P value3, OR (95% CI)		0.0010, 1.38 (1.14-1.67)
rs11209026
Genotype
RR	105 (95.45)	329 (90.38)	458 (84.97)
RQ	5 (4.54)	32 (8.79)	79 (14.66)
QQ	0	3 (0.82)	2 (0.37)
P value1	0.0120
P value2	NS
P value3		0.0220
Q allele	5 (2.27)	38 (5.22)	83 (7.69)
P value1, OR (95% CI)	0.0018, 0.28 (0.11-0.69)
P value2, OR (95% CI)	NS
P value3, OR (95% CI)		0.0400, 0.66 (0.44-0.98)

¹Childhood-onset vs controls;

²Childhood-onset vs adult onset;

³Adult onset vs controls.