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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Feb 14, 2009; 15(6): 697-704
Published online Feb 14, 2009. doi: 10.3748/wjg.15.697
Published online Feb 14, 2009. doi: 10.3748/wjg.15.697
Table 1 Real-time PCR primers for N-cadherin, E-cadherin, MMP-9 and GAPDH
Target gene | Primers | Length of product (bp) |
N-cadherin | Sense: 5’-GGTGGAGGAGAAGAAGACCAG-3’ | 72 |
Antisense: 5’-GGCATCAGGCTCCACAGT-3’ | ||
E-cadherin | Sense: 5’-CCCGGGACAACGTTTATTAC-3’ | 72 |
Antisense: 5’-GCTGGCTCAAGTCAAAGTCC-3’ | ||
MMP9 | Sense: 5’-GAACCAATCTCACCGACAGG-3’ | 67 |
Antisense: 5’-GCCACCCGAGTGTAACCATA-3’ | ||
GAPDH | Sense: 5’-AGCCACATCGCTCAGACA-3’ | 66 |
Antisense: 5’-GCCCAATACGACCAAATCC-3’ |
Table 2 Correlations of E-cadherin, N-cadherin expression with clinicopathological features of ESCC
Items | n | E-cadherin | N-cadherin | ||||
Cases (%) | χ2 | P | Cases (%) | χ2 | P | ||
Histological classification | |||||||
NEE | 62 | 59 (95.2) | 18 (29.0) | ||||
AH | 31 | 22 (71.0) | 48.426 | 0.000 | 19 (61.3) | 29.091 | 0.000 |
ESCC | 62 | 25 (40.3) | 47 (75.8) | ||||
Histological grade | |||||||
I | 15 | 11 (73.3) | 8 (53.3) | ||||
II | 25 | 9 (36.0) | 9.962 | 0.007 | 19 (76.0) | 6.924 | 0.031 |
III | 22 | 5 (22.7) | 20 (90.9) | ||||
Depth of invasion | |||||||
Not to serosa | 7 | 6 (85.7) | 4.797 | 0.029 | 2 (28.6) | 6.916 | 0.009 |
To serosa | 55 | 19 (34.5) | 45 (81.8) | ||||
Lymph node metastasis | |||||||
Yes | 42 | 21 (50.0) | 3.897 | 0.048 | 28 (66.7) | 4.486 | 0.034 |
No | 20 | 4 (20.0) | 19 (95.0) |
Table 3 Correlation between E-cadherin and N-cadherin protein expressions
E-cadherin | N-cadherin (+) | N-cadherin (-) | γ | P | |
+ | 25 | 12 | 13 | -0.534 | 0.000 |
- | 37 | 35 | 2 |
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Citation: Li K, He W, Lin N, Wang X, Fan QX. N-cadherin knock-down decreases invasiveness of esophageal squamous cell carcinoma
in vitro . World J Gastroenterol 2009; 15(6): 697-704 - URL: https://www.wjgnet.com/1007-9327/full/v15/i6/697.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.697