A simple taurocholate-induced model of severe acute pancreatitis in rats

Table 1 Pancreatitis histopathology: Schmidt Scoring Criteria

	Score
	0	1	2	3
Interstitial edema	None	Interlobular	Lobule involved	Isolated island-like acinar cells
Leukocyte infiltration	None	< 20%	20%-50%	> 50%
Acinar cell necrosis	None	< 5%	5%-20%	> 20%
Hemorrhage	None	1-2 points	3-5 points	> 20%

The Schmidt Scoring Criteria of pancreatitis pathology includes 4 aspects, including interstitial edema, inflammatory infiltration, parenchymal necrosis and hemorrhage, all of which were given scores of 0-3 according to the severity of the disease.

Table 2 Pancreatitis pathology score

Groups (n = 8)	Score
	Interstitial edema	Leukocyte infiltration	Acinar cell necrosis	Hemorrhage	Total
Control
4 d	0.13 ± 0. 33	0.13 ± 0.33	0	0	0.25 ± 0.67
7 d	0.13 ± 0.33	0	0	0	0.13 ± 0.33
SAP
4 d	1.5 ± 0.76a	2.38 ± 0.74a	1.75 ± 0.71a	0.75 ± 1.17a	6.38 ± 2.20a
7 d	1.25 ± 0.46b	2.13 ± 0.99b	1.5 ± 0.54b	0.50 ± 1.07b	5.38 ± 1.77b

In this table, the pancreatitis pathology scores in severe acute pancreatitis (SAP) rats were significantly higher than in control rats in every single aspect as well as the total score, both on the 4th day (^aP < 0.01) and on the 7th day (^bP < 0.01).

Table 3 Infection determined by the bacterial culture method

Groups (n = 8)	Infected organs number
	Blood			MLN			Pancreas			Liver			Total
	Ae	Ana	T	Ae	Ana	T	Ae	Ana	T	Ae	Ana	T	Ae	Ana	T
Control
4 d	0	0	0	0	1	1	0	0	0	0	0	0	0	1	1
7 d	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SAP
4 d	3	2	3	6	6	6	7	7	7	7	7	7	23	22	23a
7 d	3	3	3	5	6	6	7	8	8	5	5	5	20	22	22b
Total	6	5	6c	11	13	13	14	15	15	12	12	12	43	45	46

Infection rates of each organ and overall in the SAP group were higher than that in the control group, both on the 4th day (^aP < 0.01) and 7th day (^bP < 0.01); Compared with the other 3 types of organ, the infection rate of blood was lowest, ^aP < 0.05. MLN: Mesenteric lymph nodes; Ae: Aerobes; Ana: Anaerobes; T: Total.

Table 4 Bacterial translocation detected by real-time polymerase chain reaction

	Samples (n = 16)
	Blood	MLN	Pancreas	Liver
Infected number in control group	0	1	0	0
Infected number in SAP group	5a	10a	13a	12a
Bacteria number (transformed by log10)	5.43 ± 0.847	6.48 ± 1.56	7.70 ± 0.766b	6.57 ± 1.44

^aP < 0.01, compared with the “Infected number” in the control group;

^bP < 0.05, compared with the “Bacteria number (transformed by log₁₀)” in blood, MLN and liver.