Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma

Table 1 Adhesion of MHCC97-H cells to fibronectin coated microtiter plates

	20 min	40 min	60 min	90 min
Control	0.309 ± 0.16	0.432 ± 0.17	0.548 ± 0.12	0.717 ± 0.06
5 μg/mL genistein	0.181 ± 0.04a	0.267 ± 0.06a	0.341 ± 0.02	0.341 ± 0.11b
10 μg/mL genistein	0.108 ± 0.02b	0.219 ± 0.01b	0.257 ± 0.02a	0.367 ± 0.01b
20 μg/mL genistein	0.142 ± 0.07a	0.201 ± 0.04b	0.225 ± 0.04a	0.298 ± 0.09b

^aP < 0.05,

^bP < 0.01 vs control. The A₅₉₅ for MHCC97-H cells treated with or without genistein in fibronectin pre-coated 96-well microtiter plates was measured at 20, 40, 60 and 90 min. Values are A₅₉₅ mean ± SD from experiments.

Table 2 Effects of genistein on cell cycle progression in the MHCC97-H cell line

Treatment (μg/mL genistein)	Cell cycle phase			Apoptosis (%)
	G0/G1 (%)	G2/M (%)	S (%)
Control	59.12 ± 3.89	10.95 ± 6.41	29.93 ± 2.52	1.18 ± 0.01
5	67.86 ± 2.36a	4.08 ± 1.82a	28.06 ± 0.55	3.92 ± 1.63a
10	69.53 ± 2.13a	6.80 ± 0.48	23.65 ± 1.76b	7.84 ± 1.72b
20	71.85 ± 3.60a	8.84 ± 4.07	19.31 ± 1.51b	18.90 ± 4.98b

^aP < 0.05,

^bP < 0.01 vs control. Cell cycle and apoptosis were evaluated by flow cytometric analysis on a FACScan cytofluorimeter. Values are mean ± SD from experiments.