Characterization of CD133+ parenchymal cells in the liver: Histology and culture

Table 1 Age, sex, and etiology of liver diseases in our study

	n	Age (yr)	Male/Female	Etiology
Normal liver	3	50	2/1
Chronic hepatitis/cirrhosis	5	58	3/2	HBV (3), HCV (2)
HCC
Well-differentiated	3	62	2/1	HBV (1), HCV (2)
Moderately differentiated	24	62	20/4	HBV (11), HCV (7), alcohol (3), NASH (1), cryptogenic (1)1
Poorly differentiated	6	54	4/2	HBV (3)
Cholangiocarcinoma	6	60	3/3	HCV (4)
Combined carcinoma	5	59	4/1	HBV (1), HCV (4)

¹The remaining one case had no etiology of liver diseases and showed histologically normal liver. NASH: Nonalcoholic steatohepatitis; HCC: Human hepatocellular carcinoma; HBV: Hepatitis B virus; HCV: Hepatitis C virus.

Table 2 Sequences, annealing temperatures, cycle times, and product sizes of PCR primers

	F/R	Sequence	Temperature (°C)	Cycles	Size (bp)
CD1331	1st F	GCCAGAAACTGTAATCTTAG	48	35	275
	1st R	TTACCTGGTGATTTGCCACA
	2nd F	CCTGGGGCTGCTGTTTATTA	55	35	153
	2nd R	ATCACCAACAGGGAGATTGC
CK19	F	TCCCGCGACTACAGCCACTACTACACGACC	55	35	745
	R	CGCGACTTGATGTCCATGAGCCGCTGGTA
CK7	F	GGATGCTGCCTACATGAGC	52	30	164
	R	CCAGGGAGCGACTGTTGT
AFP	F	GGGAGCGGCTGACATTATTA	50	30	231
	R	TCTTGCTTCATCGTTTGCAG
Albumin	F	TGCTTGAATGTGCTGATGACAGGG	50	30	161
	R	AAGGCAAGTCAGCAGGCATCTCATC
β-actin	F	CAAGAGATGGCCACGGCTGCT	55	30	334
	R	TCCTTCTGCATCCTGTCGGCA

¹Examined by nested PCR using first and second sets of primers. F: Forward; R: Reverse.