Influence of paeonol on expression of COX-2 and p27 in HT-29 cells

Table 1 Expression of COX-2 protein in HT-29 cells after treatment with paeonol

Paeonol concentration (mmol/L)	Staining intensity	Positivity rate (%)
0	+++	98.21 ± 0.835
0.094	+++	95.82 ± 0.751a
0.376	++	47.39 ± 0.831a
1.504	+	23.60 ± 1.390a

^aP < 0.05 vs untreated control cells.

Table 2 Rate of apoptosis, cell cycle and expression of p27 after treatment with paeonol

Paeonol concentration(mmol/L)	p27 staining intensity	p27 positive rate	Rate of apoptosis	G0/G1 phase	S phase	G2/M phase
0	-	0.46	2.3	61.3	20.3	18.4
0.094	+	18.18b	7.6b	28.7b	55.8b	16.1
0.376	++	46.70b	16.1b	50.7b	35.8b	13.5b
1.504	+++	86.86b	34.5b	38.0b	45.6b	16.4

^bP < 0.01 vs untreated control cells.