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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Aug 14, 2009; 15(30): 3776-3782
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3776
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3776
Table 1 Characteristics of IBD patients
Variables | CD (n = 94) |
Boys | 44 |
Girls | 50 |
Age of onset (yr) | 11.3 ± 3.7 |
Location of disease1 | |
Terminal ileum | 11 |
Ileocolonic | 56 |
Colon | 8 |
Upper digestive tract2 | 19 |
H-B index | 5.0 ± 1.5 |
ESR (mm/h) | 39.0 ± 14 |
Albumin (g/L) | 32.0 ± 7 |
WBC (× 109/L) | 9.3 ± 3.4 |
HgB (g/L) | 110.0 ± 14 |
MCV (fL) | 76.3 ± 7.2 |
PLT (x 109/L) | 468 ± 146 |
ABS polys (%) | 6.1 ± 3 |
ABS lymphs (%) | 2.0 ± 2.1 |
ALT (IU/L) | 13.0 ± 8.9 |
AST (IU/L) | 19.5 ± 7.3 |
Table 2 Characteristic of the primers used to genotype the SNP in TNF-α, IL-10 and GR genes
Locus | SNP | Amplimers | ASO probe1 | |
TNF-α | F 5'CTAAGGAATGGAGGGAGGGA3' | |||
R 5'CTTCTGTCTCGGTTTCTTCT3' | ||||
T-1031C | rs1799964 | GAGAAGATGAAGGAA | ||
GAGAAGACGAAGGAA | ||||
C-863A | rs1800630 | GACCCCCCCTTAACG | ||
GACCCCCACTTAACG | ||||
C-857T | rs1799724 | CCCTTAATGAAGACA | ||
CCCTTAACGAAGACA | ||||
G-308A | rs59729336 | GGGCATGGGGACGGG | ||
GGGCATGAGGACGGG | ||||
G-238A | rs361525 | CGGAATCGGAGCAGG | ||
CGGAATCAGAGCAGG | ||||
IL-10 | F 5'CACTACTAAGGCTTCTTTGGG3' | |||
R 5'CTGTAGGAAGCCAGTCTCTG3' | ||||
G-1087A | rs1800896 | TTTGGGAGGGGGAAG | ||
TTTGGGAAGGGGAAG | ||||
C-824T | rs1800871 | GTGATGTAACATCTCTGTG | ||
GTGATGTAATATCTCTGTG | ||||
C-597A | rs1800872 | CGCCTGTCCTGTAGG | ||
CGCCTGTACTGTAGG | ||||
GR | R23K | F 5'TGTAGGATTGATATTCACTGATG3' | rs6189 | GGAGAGGGGAGATGT |
G > A | R 5'CAAAAGTCTTCGCTGCTTGG3' | GGAGAAGGGAGATGT | ||
N363S | F 5'TCCATGGTGTGAGTACCTCTGG3' | rs6195 | CGGTTCCGAAAGTTG | |
A > G | R 5'GACCAGGGAAGTTCAGAGTCC3' | CGGTTCCGAAAATTG |
Table 3 Allele and genotype distribution of the GR polymorphism n (%)
Position | Controls | CD |
R23K G > A | ||
GG | 79 (94) | 106 (94) |
GA | 5 (6) | 7 (6) |
AA | - | - |
G | 163 (97) | 219 (97) |
A | 5 (3) | 7 (3) |
N363S A > G | ||
AA | - | - |
AG | 5 (6) | 4 (4) |
GG | 82 (94) | 108 (94) |
A | 5 (3) | 4 (2) |
G | 169 (97) | 220 (98) |
Table 4 Distribution of GR haplotypes in patients and control groups (%)
Haplotype1 | Controls (n = 174) | CD (n = 226) |
GG | 94 | 95 |
GA | 3 | 2 |
AG | 3 | 3 |
Table 5 Allele and genotype distribution of the IL-10 polymorphisms n (%)
Position | Controls | CD |
-1087 G > A1 | ||
GG | 19 (20) | 24 (22) |
GA | 42 (45) | 50 (45) |
AA | 33 (35) | 37 (33) |
A | 108 (57) | 124 (56) |
G | 80 (43) | 98 (44) |
-824 T > C | ||
TT | 7 (7) | 14 (13) |
TC | 37 (39) | 39 (35) |
CC | 50 (53) | 64 (58) |
T | 51 (27) | 55 (25) |
C | 137 (73) | 167 (75) |
-597 A > C | ||
AA | 1 (1) | 8 (7) |
AC | 37 (39) | 40 (36) |
CC | 50 (53) | 63 (57) |
A | 51 (27) | 56 (25) |
C | 137 (73) | 166 (75) |
Table 6 IL-10 haplotypes inferred in patients and control groups (% )
Haplotype1 | Controls (n = 188) | CD (n = 222) |
GCC | 43 | 44 |
ACC | 30 | 30 |
ACA | - | 0.9 |
ATC | - | 0.45 |
ATA | 27 | 24 |
Table 8 TNF-α haplotypes inferred in patients and control groups (%)
Table 7 Allele and genotype distribution of the TNF-α polymorphisms n (%)
Position2 | Controls | CD |
-1031 C > T | ||
CC1 | 1 (1) | 12 (11) |
CT | 39 (47) | 45 (40) |
TT | 43 (52) | 55 (49) |
C | 41 (25) | 69 (31) |
T | 125 (75) | 155 (69) |
-863 A > C | ||
AA | 4 (5) | 10 (10) |
AC | 22 (28) | 32 (32) |
CC | 52 (67) | 58 (58) |
A | 30 (19) | 52 (26) |
C | 126 (81) | 148 (74) |
-857 T > C | ||
TT | 1 (1) | 1 (1) |
CT | 14 (18) | 17 (17) |
CC | 63 (80) | 82 (82) |
T | 16 (10) | 19 (10) |
C | 140 (90) | 181 (90) |
-308 A > G | ||
AA | 1 (1) | - |
AG | 27 (29) | 29 (26) |
GG | 66 (70) | 83 (74) |
A | 29 (15) | 29 (13) |
G | 159 (85) | 195 (87) |
-238 A > G | ||
AA | 2 (2) | - |
AG | 7 (8) | 11 (10) |
GG | 80 (90) | 97 (90) |
A | 11 (6) | 11 (5) |
G | 167 (94) | 205 (95) |
- Citation: Sanchez R, Levy E, Costea F, Sinnett D. IL-10 and TNF-α promoter haplotypes are associated with childhood Crohn’s disease location. World J Gastroenterol 2009; 15(30): 3776-3782
- URL: https://www.wjgnet.com/1007-9327/full/v15/i30/3776.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.3776