Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

doi:10.3748/wjg.15.3757

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 15, Issue 30

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (4399)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-6) series, Tables (1-2) series.

Item

Count

PDF

456

HTML

3310

Figures (1-6)

443

Tables (1-2)

190

Sum=4399

Aug 14, 2009 (publication date) through Nov 20, 2024

Times Cited of This Article

Times Cited (22)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Articles

World J Gastroenterol. Aug 14, 2009; 15(30): 3757-3766
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3757

Table 1 Interfering sequence specified for STAT3 gene

Number	Sequence
LV-STAT3siRNA-1	Oligo1: 5’TaaTGTTCTCTATCAGCACAATTTCAAGAGAATTGTGCTGATAGAGAACATTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaTGTTCTCTATCAGCACAATTCTCTTGAAATTGTGCTGATAGAGAACATTA3’
LV-STAT3siRNA-2	Oligo1: 5’TaaCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaCATCTGCCTAGATCGGCTATCTCTTGAATAGCCGATCTAGGCAGATGTTA3’
LV-STAT3siRNA-3	Oligo1: 5’TaaCTTCAGACCCGTCAACAAATTCAAGAGATTTGTTGACGGGTCTGAAGTTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaCTTCAGACCCGTCAACAAATCTCTTGAATTTGTTGACGGGTCTGAAGTTA 3’

Three coding regions corresponding to targeting human STAT3 starting at 466, 1638, and 1061 in the GenBank (NM 139276). Interfering sequence specified for STAT3 gene, namely LV-STAT3siRNA-1, LV-STAT3siRNA-2 and LV- STAT3siRNA-3, respectively.

Table 2 Cell cycle detected by flow cytometry (%)

Group	G1 fraction	G2 fraction	S fraction	G2 + S fraction
SW1990	35.83 ± 5.85	49.29 ± 2.80	14.45 ± 3.24	63.74 ± 5.66
LV-Con	43.34 ± 2.14	51.88 ± 3.35	4.79 ± 1.27	56.66 ± 2.14
LV-STAT3siRNA-2	52.36 ± 1.02	43.34 ± 1.14	4.30 ± 2.08	47.63 ± 1.20^a^c

Cell cycle detected by flow cytometry. The G2 + S phase fraction of the LV-STAT3siRNA-2 group was the lowest compared with LV-Con group and parental SW1990 group. This result was consistent with that of the MTT assay.

^aP < 0.05 vs SW1990 group,

^cP < 0.05 vs LV-Con group.

Citation: Yang G, Huang C, Cao J, Huang KJ, Jiang T, Qiu ZJ. Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion. World J Gastroenterol 2009; 15(30): 3757-3766
URL: https://www.wjgnet.com/1007-9327/full/v15/i30/3757.htm
DOI: https://dx.doi.org/10.3748/wjg.15.3757