Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

doi:10.3748/wjg.15.3757

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 14, 2009; 15(30): 3757-3766
Published online Aug 14, 2009. doi: 10.3748/wjg.15.3757

Table 1 Interfering sequence specified for STAT3 gene

Number	Sequence
LV-STAT3siRNA-1	Oligo1: 5’TaaTGTTCTCTATCAGCACAATTTCAAGAGAATTGTGCTGATAGAGAACATTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaTGTTCTCTATCAGCACAATTCTCTTGAAATTGTGCTGATAGAGAACATTA3’
LV-STAT3siRNA-2	Oligo1: 5’TaaCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaCATCTGCCTAGATCGGCTATCTCTTGAATAGCCGATCTAGGCAGATGTTA3’
LV-STAT3siRNA-3	Oligo1: 5’TaaCTTCAGACCCGTCAACAAATTCAAGAGATTTGTTGACGGGTCTGAAGTTTTTTTTC3’
	Oligo2: 5’TCGAGAAAAAAaaCTTCAGACCCGTCAACAAATCTCTTGAATTTGTTGACGGGTCTGAAGTTA 3’

Three coding regions corresponding to targeting human STAT3 starting at 466, 1638, and 1061 in the GenBank (NM 139276). Interfering sequence specified for STAT3 gene, namely LV-STAT3siRNA-1, LV-STAT3siRNA-2 and LV- STAT3siRNA-3, respectively.

Table 2 Cell cycle detected by flow cytometry (%)

Group	G1 fraction	G2 fraction	S fraction	G2 + S fraction
SW1990	35.83 ± 5.85	49.29 ± 2.80	14.45 ± 3.24	63.74 ± 5.66
LV-Con	43.34 ± 2.14	51.88 ± 3.35	4.79 ± 1.27	56.66 ± 2.14
LV-STAT3siRNA-2	52.36 ± 1.02	43.34 ± 1.14	4.30 ± 2.08	47.63 ± 1.20^a^c

Cell cycle detected by flow cytometry. The G2 + S phase fraction of the LV-STAT3siRNA-2 group was the lowest compared with LV-Con group and parental SW1990 group. This result was consistent with that of the MTT assay.

^aP < 0.05 vs SW1990 group,

^cP < 0.05 vs LV-Con group.

Citation: Yang G, Huang C, Cao J, Huang KJ, Jiang T, Qiu ZJ. Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion. World J Gastroenterol 2009; 15(30): 3757-3766
URL: https://www.wjgnet.com/1007-9327/full/v15/i30/3757.htm
DOI: https://dx.doi.org/10.3748/wjg.15.3757