Phenotypic changes of human cells in human-rat liver during partial hepatectomy-induced regeneration

Table 1 Phenotypic changes of donor-derived human cells in HRC liver during PHx-induced liver regeneration

Animal No. (age)		Cell markers
		AFP		CK19		CK18		CD34		CD45
		I	II	I	II	I	II	I	II	I	II
1 and 2 (2 mo)	A	-	-	+	-	-	+	-	-	-	-
	B	-	+	+	-	-	+	-	+	-	-
	C	-	-	+	-	-	+	-	-	-	-
3 and 4 (2 mo)	A	-	-	+	+	+	-	+	-	-	-
	B	-	-	+	+	+	-	+	+	-	+
	C	-	-	+	+	+	+	-	-	-	+
5 and 6 (2 mo)	A	-	-	+	+	+	-	-	-	-	-
	B	-	+	+	+	+	+	-	+	-	+
	C	-	-	+	+	+	+	-	-	-	-
7 and 8 (2 mo)	A	-	-	-	+	+	+	-	-	-	-
	B	-	-	-	+	+	+	-	-	-	-
	C	-	-	-	+	+	+	-	-	-	-
9 and 10 (2 mo)	A	-	-	-	-	+	+	-	-	-	-
	B	-	-	-	-	+	+	-	-	-	+
	C	-	-	-	+	+	+	-	-	-	-

Animals 1-10 at the age of 2 mo with the engraftment of donor-derived hHLCs in the liver of HRC were screened by human gene-specific PCR for hAlu as previously described[1920] and ISH for human Alu sequences confirmed by detecting human β2-M expression using IHC. Animals 2, 4, 6, 8 and 10 at the age of 2 mo underwent 70% PHx. Age-matched animals 1, 3, 5, 7 and 9 (not undergone PHx), were used as negative controls. In the human-rat chimeric liver, donor-derived human cells with different cellular phenotypes were detected by IHC for different markers (AFP, CD34, CD45, CK18 and CK19). Minimal liver tissues from PHx (II) and non-PHx (I) groups used for IHC were dissected and collected before PHx (A), and after 10 d (B) and 20 d (C) of PHx.