Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR

Table 1 Etiologic diagnoses of neonatal intrahepatic cholestasis (NIHC)

Etiology	Number of cases
Neonatal sepsis	9
Cytomegalovirus	5
Urinary infection tract	4
Syphilis	2
Toxoplasmosis	2
α1-antitripsin deficiency	5
Other metabolic diseases	5
Galactosemia	4
Allagile’s syndrome	2
Byler’s disease	1
Cystic fibrosis	1
Secondary to use of parenteral nutrition	1
Down’s syndrome	1
Panhypopituitarism	1
Idiopathic	58
Total	101

Table 2 Serological results (IgM-ELISA), presence of cytomegalic cells (histological analysis), IHC, and PCR

Methods	Positive	n1 (%)
IgM-ELISA	7	64 (11)
Histological analysis	0	84 (0)
IHC	1	44 (2)
PCR	6	77 (8)

¹Number of patients submitted to each diagnostic method.

Table 3 Clinical and laboratory data of CMV-positive patients by PCR: gender, diagnosis, weight at birth, serology (IgM-ELISA), IHC, and presence of cytomegalic cells

Patients	Gender	Diagnosis	Weight at birth (g)	IgM-ELISA	IHC	Cytomegalic cell
1	M	Cystic fibrosis	3460	Positive	-	Absent
2	F	Idiopathic	2500	Negative	Negative	Absent
3	M	Byler’s disease	3320	Positive	Positive	Absent
4	M	Idiopathic	2700	Negative	-	Absent
5	F	Idiopathic	1500	Negative	Negative	Absent
6	M	Neonatal sepsis	2570	Negative	-	Absent

-: Not performed.

Table 4 Results of PCR and serology (IgM-ELISA) and number of patients tested

IgM-ELISA	PCR
	+	-	Total
+	2	5	7
-	4	40	44
Total	6	45	51

Table 5 Results of positive predictive value (PV+), negative predictive value (PV-), accuracy, sensitivity, and specificity between PCR and serology with 95% confidence intervals (CI)

	Percent	CI (95%)
		Inferior limit	Superior limit
Sensibility	33.3	6.00	75.89
Specificity	88.89	75.15	95.84
PV+	28.57	5.11	69.74
PV-	90.91	77.42	97.05
Accuracy	82.35	68.64	91.13