Comparative identification of Ca2+ channel expression in INS-1 and rat pancreatic β cells

Table 1 Sequences of primer pairs of Ca²⁺ channel subunits and RT-PCR

Primer	Sequence	Temperature (°C)
L-α1C (5')	5'-GTCCATAGTGGGTCGTCT-3'	50
L-α1C (3')	5'-TGGTTTGTTCTTGCTTTC-3'
L-α1D (5')	5'-CAGGGATGCTGTGGAAGT-3'	51
L-α1D (3')	5'-TGGGCTGAGAACCTAGACG-3'
L-α-S (5')	5'-ATGCCAGAGGATGACAACAAC-3'	55
L-α-S (3')	5'-CACCCAgAAAgACAATGATgAA-3'
L-α1F (5')	5'-GACGGCAACTTGGCTTCT-3'	53
L-α1F (3')	5'-GCTGGCATGACTGCTGGT-3'
N-α1β(5')	5'-GCTCGCTCTTCGTCTTCA-3'	52
N-α1β (3')	5'-AGGTTCCGTGTCATCCAGT-3'
R-α1E (5')	5'-ATGTCCCTGAAGATGTATGG-3'	50
R-α1E (3')	5'-AACGACCTCAAAGATGCTG-3'
Ryr1 (5')	5'-GGGCGGAGAATGAGAAAGA-3'	55
Ryr1 (3')	5'-CAGGGTCGTCACGGTTGT-3'
Ryr2 (5')	5'-TGAGTATGCCCATTGAGT-3'	48
Ryr2 (3')	5'-CTTTGCTTTAGGCGTGAG-3'
TRPC1 (5')	5'-GTCAGACATTAAGAGGCTGTG-3'	50
TRPC1 (3')	5'-AAGTTGCCAAGTAAAGGGA-3'
TRPC4α (5')	5'-AATGGTTCTGCCCTGGTG-3'	50
TRPC4α (3')	5'-GAAGATTTGGTTTGCGTTT-3'
TRPC4β (5')	5'-GCAGCATTCCTGGTCTCA-3'	51
TRPC4β (3')	5'-GGGCGTGTTTCTCCTTTG-3'
TRPM2 (5')	5'-GTCATCACCATCGGCATAGC-3'	56
TRPM2 (3')	5'-TGTCCAGGCAGGTCAGGTT-3'
IP3R1 (5')	5'-CAACCGTTACTATGGAAACATC-3'	54
IP3R1 (3')	5'-TCAGCCAGGCTCATCTCAC-3'
IP3R2 (5')	5'-CGATGCCAGGATACGATGT-3'	54
IP3R2 (3')	5'-CACCCTTGAAGTACCGATT-3'
IP3R3 (5')	5'-AGGAGCTGGTGGACGTGAT-3'	55
IP3R3 (3')	5'-TGCTTGTTGTGCCTGGAAA-3'

Table 2 Expression profile and clone numbers of Ca²⁺ channel subunits in INS-1 and rat primary β cells (mean ± SE)

Ca2+ channel subunits	Length of PCR production (bp)	INS-1	β cells
L-α1C	147	1.767 × 107± 1.763 × 106	3.836 × 106± 1.087 × 106
L-α1D	197	1.046 × 106± 2.074 × 104	1.913 × 106± 1.329 × 105
L-α-S1	181	-	-
L-α1F1	144	-	-
N-α1β	188	1.078 × 104± 6.477 × 102	6.832 × 103± 3.220 × 102
R-α1E	102	6.614 × 103± 1.477 × 102	2.84 × 103± 1.943 × 102
Ryr11	159	-	-
Ryr2	250	1.367 × 104± 7.213 × 10	6.520 × 105± 4.864 × 104
TRPC1	101	5.631 × 104± 1.904 × 103	3.260 × 106± 2.531 ×105
TRPC4α	196	8.033 × 104± 5.405 × 103	2.073 × 106± 2.207 × 105
TRPC4β	165	2.226 × 105± 7.672 × 104	6.666 × 106± 1.595 × 106
TRPM2	130	1.028 × 106± 9.696 × 104	1.538 × 106± 2.476 × 105
IP3R1	159	1.189 × 105± 1.650 × 104	1.922 × 106± 7.584 × 105
IP3R2	191	3.339 × 104± 7.945 × 103	8.293 × 104± 7.041 × 103
IP3R3	130	5.613 × 105± 1.009 × 105	7.863 × 105± 3.894 × 103

¹These three subunits were not identified in INS-1 and rat β cells.