Biochemical metabolic changes assessed by 31P magnetic resonance spectroscopy after radiation-induced hepatic injury in rabbits

Table 1 The criteria of liver damage score

Grade	Albumin (g/L)	γ-globulins (g/L)	AST (U/L)	Conjugated bilirubin (&mgr;mol/L)	Creatinine (&mgr;mol/L)
0	> 36.5	< 19.9	< 50	< 6	< 119
1	32.9-36.4	20-26	51-180	7-32	120-150
2	28.5-32.8	26.1-34.9	181-384	33-75	151-230
3	24.5-28.4	> 35	> 385	> 76	> 231
4	21.8-24.4
5	< 21.7

Table 2 Relative quantification of metabolites in rabbit liver using the LDS evaluation method (mean ± SD)

Metabolite	Control group (n = 10)	LDS-mild group (n = 13)	LDS-moderate group (n = 9)	LDS-severe group (n = 8)	F	P
PME	0.86 ± 0.23j	0.79 ± 0.33	0.80 ± 0.22	0.58 ± 0.22a	1.852	0.155
PDE	2.27 ± 0.62	2.14 ± 0.51	2.48 ± 0.63	1.99 ± 0.88	3.023	0.042
Pi	0.74 ± 0.18j	0.63 ± 0.28	0.62 ± 0.29	0.43 ± 0.14a	3.475	0.026
ATP	1.83 ± 0.33eil	1.55 ± 0.24bhl	1.27 ± 0.09cej	0.98 ± 0.18cfg	22.647	0.000
PME/PDE	0.36 ± 0.12	0.39 ± 0.18	0.35 ± 0.14	0.44 ± 0.54	0.462	0.710
PME/ATP	0.50 ± 0.11	0.50 ± 0.18	0.62 ± 0.15	0.61 ± 0.25	0.990	0.409
PDE/ATP	1.43 ± 0.31gj	1.40 ± 0.38gj	1.94 ± 0.50ad	1.96 ± 0.72ad	5.080	0.005
Pi /ATP	0.40 ± 0.09	0.41 ± 0.19	0.49 ± 0.24	0.46 ± 0.16	1.582	0.211
PME/Pi	1.47 ± 0.65	1.49 ± 0.92	1.76 ± 1.12	1.39 ± 0.58	0.081	0.970
PDE/Pi	3.46 ± 1.15	4.26 ± 2.58	4.91 ± 2.65	5.05 ± 3.18	0.902	0.450

^aP < 0.05,

^bP < 0.01,

^cP < 0.005 vs control group;

^dP < 0.05,

^eP < 0.01,

^fP < 0.005 vs mild group;

^gP < 0.05,

^hP < 0.01,

ⁱP < 0.005 vs moderate group;

^jP < 0.05, ^kP < 0.01,

^lP < 0.005 vs severe group.

Table 3 Relative quantification of metabolites in rabbit liver with the pathological evaluation method

Metabolite	Control group (n = 10)	Pathological mild group (n = 12)	Pathological moderate group (n = 11)	Pathological severe group (n = 7)	F	P
PME	0.86 ± 0.23j	0.82 ± 0.35	0.79 ± 0.24	0.60 ± 0.21a	1.861	0.154
PDE	2.27 ± 0.62	2.08 ± 0.47g	2.67 ± 0.38dk	1.92 ± 0.83h	1.118	0.355
Pi	0.74 ± 0.18k	0.61 ± 0.24	0.7 ± 0.33j	0.43 ± 0.14bg	2.334	0.090
ATP	1.83 ± 0.33dil	1.58 ± 0.25agl	1.32 ± 0.07cdk	1.02 ± 0.18cfh	22.878	0.000
PME/PDE	0.36 ± 0.12	0.40 ± 0.18	0.31 ± 0.13	0.45 ± 0.48	0.189	0.903
PME/ATP	0.50 ± 0.11h	0.51 ± 0.19i	0.60 ± 0.18bf	0.59 ± 0.22	1.412	0.255
PDE/ATP	1.43 ± 0.31h	1.34 ± 0.33ij	2.04 ± 0.36bf	1.83 ± 0.72d	4.082	0.014
Pi/ATP	0.40 ± 0.09	0.39 ± 0.15	0.54 ± 0.26	0.43 ± 0.16	0.601	0.619
PME/Pi	1.47 ± 0.65	1.57 ± 0.97	1.44 ± 1.03	1.61 ± 0.75	0.316	0.814
PDE/Pi	3.46 ± 1.15	4.12 ± 2.36	4.87 ± 2.63	5.09 ± 3.24	0.810	0.497

^aP < 0.05,

^bP < 0.01,

^cP < 0.005 vs control group;

^dP < 0.05, ^eP < 0.01,

^fP < 0.005 vs mild group;

^gP < 0.05,

^hP < 0.01,

ⁱP < 0.005 vs moderate group;

^jP < 0.05,

^kP < 0.01,

^lP < 0.005 vs severe group.