Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates

doi:10.3748/wjg.15.204

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 14, 2009; 15(2): 204-210
Published online Jan 14, 2009. doi: 10.3748/wjg.15.204

Table 1 Primer used for PCR genotyping of Cuban H pylori strains

Primer	Sequence (5’-3’)	AT °C	Size (bp)	Ref.
glmMF	CCCTCACGCCATCAGTCCCAAAAA	60	417	[18]
glmMR	AAGAAGTCAAAAACGCCCCAAAAC
cagF1	GATAACAGGCAAGCTTTTGA	60	349	[7]
cagB1	CTGCAAAAGATTGTTTGGCAGA
vacAsF	ATGGAAATACAACAAACACAC	52	s1-259/s2-286	[20]
vacAsR	CTGCTTGAATGCGCCAAAC
vacAmF	CAATCTGTCCAATCAAGCGAG	56	m1-567/m2-642	[20]
vacAmR	GCGTCAAAATAATTCCAAGG
bab7-F	CCAAACGAAACAAAAAGCGT	60	271	[21]
bab7-R	GCTTGTGTAAAAGCCGTCGT
babA2F¹	AATCCAAAAAGGAGAAAAAGTATGAAA	60	832	[13]
babA2R	TGTTAGTGATTTCGGTGTAGGACA
babA2R607²	GTTTTCTTTGAGCGCGGGTAAGC	60	607	[14]

¹Forward primer used with primer babA2R or babAR607 to amplify babA2 gene;

²Five nucleotides (GTTTT) were added to the original primer designed by Zambon et al[14] to increase specificity.

Table 2 Correlation between vacA alleles and cagA and babA2 genotypes in 130 Cuban H pylori isolates

vacA	*s1m1*	s1m2	s2m2	s2m1	s1m-	Total (%)
cagA+	70	14	8	1	2	95 (73.2)
cagA-	4	5	25	0	1	35 (26.8)
babA2+	67	14	24	1	1	107 (82.3)
babA2-	7	5	9	0	2	23 (17.7)
Total (%)	74 (56.9)	19 (14.6)	33 (35.4)	1 (0.8)	3 (2.3)	130

Table 3 Correlation between virulence factor genotypes and disease outcome

Genotypes	Pathologies
Genotypes	FD n = 51 (%)	GU n = 33 (%)	DU n = 46 (%)
vacAs1m1	28 (54.9)	16 (48.5)	30 (65.2)
s1m2	5 (9.7)	9 (27.3)	5 (10.9)
s2m2	16 (31.4)	6 (18.2)	11 (23.9)
s1m-	1 (2)	2 (6)	-
s2m1	1 (2)	-	-
cagA+	36 (70.6)	19 (57.6)	40 (87)
babA2+	36 (70.6)	28 (84.8)	43 (93.5)^b
Type 1	29 (56.9)	16 (48.5)	40 (87)^d
Triple-positive	24 (47.1)	12 (36.4)	37 (80.4)^f

FD: Functional dyspepsia; GU: Gastric Ulcer; DU: Duodenal Ulcer; P values were calculated with the χ² test; ^{b, d, f}Statistically significant differences (P values < 0.01).

Table 4 Worldwide distribution of main H pylori virulence factors

World Area	vacA alleles prevalence (%)				References	cagAprevalence (%) cagA+	babA2prevalence (%) babA2+
World Area	s1	s2	m1	m2	References	cagAprevalence (%) cagA+	babA2prevalence (%) babA2+
Europe	48-89	11-51	37	63	[14 23 26 27]	66-73[14 23 26 27]	34-72[13 14 34]
America	57-68	16-48	37-44	29-63	[19 20 24]	57-75[7 19 24]	46-69[24 32]
East Asia	100	0	41-94	5-55	[12 25 28]	90-100[12 25 28 35]	80-100[12 21 35]

Citation: Torres LE, Melián K, Moreno A, Alonso J, Sabatier CA, Hernández M, Bermúdez L, Rodríguez BL. Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates. World J Gastroenterol 2009; 15(2): 204-210
URL: https://www.wjgnet.com/1007-9327/full/v15/i2/204.htm
DOI: https://dx.doi.org/10.3748/wjg.15.204