Original Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. May 7, 2009; 15(17): 2097-2108
Published online May 7, 2009. doi: 10.3748/wjg.15.2097
Table 1 Primers used for quantitative and qualitative PCR
GeneForwardReverse
hGAPDHGAAGGTGAAGGTCGGAGTCATGGGTGGAATCATATTGGAA
hCSECACTGTCCACCACGTTCAAGGTGGCTGCTAAACCTGAAGC
hCSE-IR1CATTACAGAGTTCAGTGTACCTGCAGCTTGGATTCTCATCAGTC
r18SGCAATTATTCCCCATGAACGGGCCTCACTAAACCATCCAA
rCSEGTATTGAGGCACCAACAGGTGTTGGGTTTGTGGGTGTTTC
rFXRTGGACTCATACAGCAAACAGAGAGTCTGAAACCCTGGAAGTCTTTT
rαSMAGCTCCATCCTGGCTTCTCTATAGAAGCATTTGCGGTGGAC
rCOL1α1TGCTGCCTTTTCTGTTCCTTGGATTTGAAGGTGCTGGGTA
rSHPCCTGGAGCAGCCCTCGTCTCAGAACACTGTATGCAAACCGAGGA
m18SACCGCAGCTAGGAATAATGGAGCCTCAGTTCCGAAAACCA
mCSETGCTGCCACCATTACGATTAGATGCCACCCTCCTGAAGTA
mα1-collagenACGTCCTGGTGAAGTTGGTCCAGGGAAGCCTCTTTCTCCT
Table 2 Effect of loss of FXR on liver injury induced by 12 administrations of CCl4 (6 wk)
AST (U/L)ALT (U/L)Bilirubin (mg/dL)
FXR +/+ naive127 ± 1548 ± 80.015 ± 0.001
FXR +/+ CCl4250 ± 40a369 ± 35a0.118 ± 0.004a
FXR -/- naive143 ± 1079 ± 25a0.068 ± 0.004a
FXR -/- CCl4 740 ± 230ce354 ± 137c0.252 ± 0.02ce