Effect of Bu-Zhong-Yi-Qi-Tang on deficiency of N-glycan/nitric oxide and islet damage induced by streptozotocin in diabetic rats

doi:10.3748/wjg.15.1730

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Apr 14, 2009 (publication date) through Mar 2, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 14, 2009; 15(14): 1730-1737
Published online Apr 14, 2009. doi: 10.3748/wjg.15.1730

Table 1 Changes in histomorphometry of pancreatic islets of diabetic rats after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)

Treatment	Islet number islets/pancrease section	Islet size cells/islet section	Necrosis	Atrophy	Cell infiltration
Normal rats	3.50 ± 0.84	3.83 ± 0.45	0	0.17 ± 0.41	0
Diabetic rats	1.08 ± 0.29^b	1.00 ± 0.0^b	3.25 ± 0.45^b	2.08 ± 0.29^a	0
Bu-Zhong-Yi-Qi-Tang-treated rats	1.17 ± 0.41^b	2.5 ± 0.55^b^d	0.33 ± 0.52^d	1.17 ± 0.41^a^d	0

^aP < 0.05,

^bP < 0.01 vs normal control rats;

^dP < 0.01 vs diabetic rats.

Table 2 Levels of nitrite and nitrate in serum and pancreas tissue of diabetic rats after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)

Treatment	Nitrite &mgr;mol/L serum	Nitrate &mgr;mol/L serum	Nitrite &mgr;mol/L per gram wet tissue	Nitrate &mgr;mol/L per gram wet tissue
Normal rats	21.33 ± 0.25	98.78 ± 49.20	146.70 ± 24.77	13.29 ± 10.73
Diabetic rats	14.00 ± 0.19^b	85.76 ± 30.08	95.60 ± 28.89^b	11.59 ± 9.54
Bu-Zhong-Yi-Qi-Tang-treated rats	25.98 ± 0.74^b^d	114.78 ± 30.09^d	132.82 ± 54.03^d	21.30 ± 18.73

^aP < 0.05,

^bP < 0.01 vs normal rats;

^dP < 0.01 vs diabetic rats.

Table 3 Activity of α-mannosidase and amylase in serum and pancreas tissue of diabetic group after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)

Treatment	Serum enzyme activities (U/mL serum)		Tissue enzyme activities (U/g wet tissue)
	α-mannosidase	Amylase	α-mannosidase	Amylase
Normal rats	3.60 ± 2.43	28.71 ± 11.07	81.78 ± 30.34	19.53 ± 3.84
Diabetic rats	3.17 ± 7.07	17.35 ± 13.76^a	20.26 ± 20.42^b	13.15 ± 0.98^a
Bu-Zhong-Yi-Qi-Tang-treated rats	1.53 ± 1.72	25.14 ± 8.76^c	44.79 ± 26.93^b^d	17.94 ± 2.20^d

^aP < 0.05,

^bP < 0.01 vs normal rats;

^cP < 0.05,

^dP < 0.01 vs diabetic rats.

Table 4 Binding of lectin to amylase in serum of diabetic group after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)

Treatment	ConA-binding% (0-binding%)	LCA-binding% (0-binding%)	PNA-binding% (0-binding%)	PSA-binding% (0-binding%)
Normal rats	22.18 ± 42.27 (71.43)	2.61 ± 6.78 (85.71)	3.89 ± 9.92 (85.71)	6.52 ± 14.22 (78.57)
Diabetic rats	31.18 ± 43.91 (58.33)	27.84 ± 44.15^a (58.33)	32.86 ± 41.01^a (50.0)	25.0 ± 45.23 (75.0)
Bu-Zhong-Yi-Qi-Tang-treated rats	1.20 ± 3.62^a^c (87.50)	16.41 ± 34.06 (75.0)	1.78 ± 5.0^c (87.50)	9.09 ± 16.44 (62.50)

^aP < 0.05 vs normal rats;

^cP < 0.05 vs diabetic rats.

Table 5 Binding of of lectin to amylase in pancreas tissue of diabetic group after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)

Treatment	ConA-binding% (0-binding%)	LCA-binding% (0-binding%)	PSA-binding% (0-binding%)
Normal rats	23.53 ± 27.83 (40.0)	7.06 ± 15.78 (80.0)	20.0 ± 44.72 (80.0)
Diabetic rats	20.0 ± 35.46 (62.5)	47.30 ± 40.53^a (12.5)	14.87 ± 27.68 (75.0)
Bu-Zhong-Yi-Qi-Tang-treated rats	16.82 ± 31.18 (50.0)	35.91 ± 37.08^a (30.0)	22.0 ± 41.46 (50.0)

^aP < 0.05 vs normal rats.

Table 6 Correlation between the number, size, necrosis and atrophy of islets and levels of α-mannosidase, amylase, nitrite and nitrate and binding of lectin to amylase

Biochemistry index	n	Islet number	Islet size	Necrosis	Atrophy
Serum nitrite	50	0.404^b	0.736^b	-0.319^b	-0.866^b
Serum nitrate	50	0.181	0.194	-0.126	-0.245^a
Pancreas tissue nitrite	50	0. 30^a	0.456^b	-0.222	-0.480^b
Pancreas tissue nitrate	50	0.101	0.157	-0.041	-0.317^b
LCA-binding of amylase in serum	61	-0.254^a	-0.290^a	0.077	0.228^a
ConA-binding of amylase in serum	61	-0.048	-0.10	-0.011	-0.016
PNA-binding of amylase in serum	61	-0.114	-0.261^a	0.283^a	0.041
PSA-binding of amylase in serum	61	-0.143	-0.26^a	0.165	0.153
LCA-binding of amylase in pancreas tissue	43	-0.442^b	-0.253^a	0.174	0.183
ConA-binding of amylase in pancreas tissue	43	-0.117	-0.095	-0.192	0.232
PSA-binding of amylase in pancreas tissue	43	-0.095	-0.037	-0.288^a	0.106
The activity of α-mannosidase in serum	61	0.067	0.050	0.026	-0.108
The activity of α-mannosidase in pancreas tissue	43	0.638^b	0.747^b	-0.508^b	-0.714^b
The activity of amylase in serum	61	0.158	0.365^b	-0.215^a	-0.310^b
The activity of amylase in pancreas tissue	43	0.428^b	0.639^b	-0.314^a	-0.631^b

^aP < 0.05,

^bP < 0.01, test of significance of correlation coefficient.

Citation: Liu XQ, Wu L, Guo XJ. Effect of Bu-Zhong-Yi-Qi-Tang on deficiency of N-glycan/nitric oxide and islet damage induced by streptozotocin in diabetic rats. World J Gastroenterol 2009; 15(14): 1730-1737
URL: https://www.wjgnet.com/1007-9327/full/v15/i14/1730.htm
DOI: https://dx.doi.org/10.3748/wjg.15.1730