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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Mar 28, 2009; 15(12): 1465-1471
Published online Mar 28, 2009. doi: 10.3748/wjg.15.1465
Published online Mar 28, 2009. doi: 10.3748/wjg.15.1465
Table 1 Epidemiological characteristics of the control group and patients n (%)
Demographic data | Control | Gastritis | DU | GU | AC |
n= 100 | n= 80 | n= 33 | n= 34 | n= 30 | |
Age (yr) | |||||
> 50 | 31 | 26 (32) | 11 (33) | 12 (35) | 21 (70) |
< 50 | 69 | 54 (68) | 22 (67) | 22 (65) | 9 (30) |
P value | NS | NS | NS | 0.00 | |
Sex | |||||
Male | 57 | 39 (49) | 19 (58) | 21 (62) | 19 (63) |
Female | 43 | 41 (51) | 14 (42) | 13 (38) | 11 (37) |
P value | NS | NS | NS | NS | |
IgG-anti H pylori | |||||
Positive | 61 | 74 (92) | 30 (91) | 33 (97) | 29 (97) |
Negative | 39 | 6 (8) | 3 (9) | 1 (3) | 1 (3) |
P value | 0.00 | 0.00 | 0.00 | 0.00 | |
anti-CagA IgG (HP+) | |||||
Positive | 38 | 53 (66) | 29 (97) | 31 (94) | 28 (97) |
Negative | 23 | 21 (34) | 1 (3) | 2 (6) | 1 (3) |
P value | NS | 0.00 | 0.00 | 0.00 |
Table 2 Comparison of genotype frequencies for the polymorphisms of IL-1β genes studied with results of other studies in different ethnic and population groups n (%)
Genotypes | Pará (Brazil) | Minas Gerais (Brazil) | Caucasians | Asians |
IL-1β-31 | ||||
TT | 9 | 102 (35.8)1 | 7 (12)2 | 7 (4)4 |
TC | 23 | 138 (48.4) | 21 (36) | 34 (20) |
CC | 68 | 45 (15.8) | 30 (52) | 128 (76) |
P value | Reference | 0.001 | NS | NS |
IL-1β-511 | ||||
CC | 31 | 108 (37.9)1 | 29 (50)2 | 34 (20)4 |
CT | 46 | 137 (48.1) | 21 (36) | 97 (57) |
TT | 23 | 40 (14) | 8 (14) | 38 (23) |
P value | Reference | NS | NS | NS |
IL-1RN | ||||
11 | 58 | 175 (61.4)1 | 37 (64)2 | 163 (96)4 |
12 | 36 | 92 (32.3) | 14 (24) | 4 (3) |
22 | 6 | 18 (6.3) | 17 (10) | 2 (1) |
P value | Reference | NS | NS | 0.001 |
TNF-α-308 | ||||
GG | 86 | 223 (78.3)1 | 152 (72)3 | 274 (91.3)5 |
GA | 13 | 54 (18.9) | 52 (25) | 24 (8) |
AA | 1 | 8 (2.8) | 6 (3) | 2 (0.7) |
P value | Reference | NS | 0.025 | NS |
Table 3 Distribution of genotypes for IL-1β (-511 and -31), IL-1RN and TNF-α in the control group and in patients with gastrointestinal pathologies n (%)
Genotypes | Control | Gastritis | DU | GU | AC |
n= 100 | n= 80 | n= 33 | n= 34 | n= 30 | |
IL-1β-31 | |||||
T/T | 9 | 3 (4) | 1 (3) | 2 (6) | 1 (4) |
C/T | 23 | 21 (26) | 9 (27) | 7 (20) | 4 (13) |
C/C | 68 | 56 (70) | 23 (70) | 25 (74) | 25 (83) |
P value | NS | NS | NS | NS | |
IL-1β-511 | |||||
C/C | 31 | 26 (33) | 9 (27) | 10 (29) | 6 (20) |
C/T | 46 | 34 (42) | 13 (39) | 14 (41) | 11 (37) |
T/T | 23 | 20 (25) | 11 (34) | 10 (30) | 13 (43) |
P value | NS | NS | NS | NS | |
IL-1RN | |||||
1/1 | 58 | 41 (51) | 15 (46) | 11 (32) | 10 (33) |
1/2 | 36 | 34 (43) | 16 (49) | 21 (62) | 19 (63) |
2/2 | 6 | 5 (6) | 2 (6) | 2 (6) | 1 (4) |
P value | NS | NS | 0.00 | 0.00 | |
TNF-α | |||||
G/G | 86 | 62 (77) | 23 (70) | 29 (85) | 24 (80) |
G/A | 13 | 16 (20) | 8 (24) | 4 (12) | 5 (16) |
A/A | 1 | 2 (3) | 2 (6) | 1 (3) | 1 (4) |
P value | NS | NS | NS | NS |
Table 4 Combined risk of polymorphism in the IL1-RN gene and IgG CagA antibody for development of gastric ulcers and adenogastric carcinoma
IL1-RN | CagA | Control | GU | ORa (95% IC) | P | AC | ORb (95% IC) | P |
1/1 | (-) | 15 (24%) | 2 (6%) | Ref. | - | 1 (4%) | Ref. | - |
2* carrier | (-) | 8 (13%) | 2 (6%) | 1.875c | 0.983 | 1 (4%) | 1.875c | 0.735 |
1/1 | (+) | 21 (35%) | 9 (27%) | 3.214 (0.605-17.063) | 0.289 | 8 (30%) | 5.714 (0.644-50.648) | 0.185 |
2* carrier | (+) | 17 (28%) | 20 (61%) | 8.823 (1.762-44.181) | 0.008 | 19 (62%) | 16.764 (1.997-140.707) | 0.004 |
Total | 61 | 33 | 29 |
Table 5 Association of polymorphism for IL-RN gene and anti-CagA antibodies with histopathological findings from patients
IL1-RN | CagA | DI | OR (95% CI) | P | NA | OR (95% CI) | P | Metaplasia | OR (95% CI) | P | |||
1 | 2 and 3 | 1 | 2 and 3 | + | - | ||||||||
1/1 | (-) | 13 | 2 | - | - | 12 | 3 | - | - | 1 | 14 | - | - |
2* carrier | (-) | 9 | 1 | 0.722a | 0.706 | 8 | 2 | 1a | 0.609 | 2 | 8 | 3.5a | 0.706 |
1/1 | (+) | 24 | 34 | 9.2 00 (1.90-44.606) | 0.004 | 26 | 32 | 4.923 (1.254-19.314) | 0.032 | 17 | 41 | 5.804 (0.706-47.693) | 0.139 |
2* carrier | (+) | 20 | 63 | 20.475 (4.253-98.556) | 0.001 | 23 | 60 | 10.434 (2.695-40.388) | 0.003 | 35 | 58 | 8.448 (1.064-67.065) | 0.038 |
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Citation: Melo Barbosa HP, Martins LC, dos Santos SEB, Demachki S, Assumpção MB, Aragão CD, Corvelo TCO. Interleukin-1 and TNF-α polymorphisms and
Helicobacter pylori in a Brazilian Amazon population. World J Gastroenterol 2009; 15(12): 1465-1471 - URL: https://www.wjgnet.com/1007-9327/full/v15/i12/1465.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.1465