Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Table 1 Chromosome aberrations detected by SKY in LST-R1 cell line

64-66,XX,+der(1)t(1;3)(p11;?)t(3;19)(?;?)[21],der(2)t(2;6)(p11;q23)[30],der(2)t(2;22)(p21;?)t(3;22)(?;?)t(2;3)(?;?)[9],der(2)t(2;22)(p21;?)t(3;22)(?;?)t(2;3)(?;?)t(2;3)(q24;?)[21],-2[30],del(3)(p24)[30],-3[9],der(3)del(p12)t(2;3)(q21;q11)[21],-3[9],der(4)t(4;10)(q11;p11)[30],der(4)dup(14)(q22q31)t(4;14)(q?;q?)[9],der(4)t(3;4)(p21;p16)dup(14)(q22q31)t(4;14)(q?;q?)[21],-4[30],der(5;8)(p10;p10)[30],+i(5)(p10)×2[30],+der(5;20)(p10;q10)del(5)(q31)[30],der(6)t(2:6)(p13;q23)[30],der(7)t(7;15)(p22;q?)[30],der(7)t(7;17)(p13;?)[29],der(8)(3;8)(?;q22)[30],der(9)t(9;19)(p11;?)[30],+der(9)t(9;19)(q11;?)[30],+r(9)(p13q13)[29],der(10;13)(q10;q10)[30],der(10)t(4;10)(p11;11)t(4;8)(q31;q13)t(3;10)(p23;q23)[30],der(12)t(3:12)(q24;q11)[30],der(12)t(5;12)(?;p11)[30],+der(12)t(2;12)(q22;q11)[30],+der(12)t(2;12)(p?;p11)t(2;12)(q24;q?)[30],+der(12)t(12;20)(q24;?)[30],-13[30],-14[30],+der(15)t(6;15)(?;p11)[30],der(17)t(15:17)(q25;q24)[30],-18[30],-19[30],-19[30],-21[30],der(22)t(3;22)(?;p13)[9],-22[30][cp30]

Table 2 Genes differentially expressed in LST cells when compared with SW480 cells are functionally categorized based on the Gene Ontology terms available from the online batch extract interface for SOURCE. (http://smd.stanford.edu/cgi-bin/source/sourceBatchSearch). This table shows the genes categorized in cell adhesion

Accession	Gene name	Gene symbol	Fold change
Z24725	Mitogen inducible 2	MIG2	3.21
Z22555	CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1	CD36L1	3.10
S80990	Ficolin (collagen/fibrinogen domain-containing) 1	FCN1	2.91
AL137480	Formin binding protein 4	FNBP4	2.73
AL117505	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2	ATP2A2	2.63
M26683	Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)	SCYA2	2.45
AF055084	Monogenic, audiogenic seizure susceptibility 1 homolog (mouse)	MASS1	2.39
X68264	Melanoma cell adhesion molecule	MCAM	2.38
AF052124	Secreted phosphoprotein 1 (osteopontin, bone sialoproteinI, early T-lymphocyte activation 1)	SPP1	2.31
M25280	Selectin L (lymphocyte adhesion molecule 1)	SELL	2.23
M16336	CD2 antigen (p50), sheep red blood cell receptor	CD2	2.19
AL161973	Myeloid/lymphoid or mixed-lineage leukemia [trithorax (Drosophila) homolog]; translocated to, 4	MLLT4	2.14
L25286	Collagen, type XV, alpha 1	COL15A	2.10
D83018	Nel (chicken)-like 2	NELL2	-48.1
AB040902	Fibronectin leucine rich transmembrane protein 3	FLRT3	-3.51
AA460440	CD164 antigen, sialomucin	CD164	-2.23
U85611	Calcium and integrin binding protein (DNA-dependent protein kinase interacting protein)	CIB1	-2.03

Table 3 Genes differentially expressed in LST cells when compared with LoVo cells are functionally categorized based on the Gene Ontology terms available from the online batch extract interface for SOURCE. (http://smd.stanford.edu/cgi-bin/source/sourceBatchSearch). This table shows the genes categorized in cell adhesion

Accession	Gene name	Gene symbol	Fold change
S80990	Ficolin (collagen/fibrinogen domain-containing) 1	FCN1	42.87
AL161973	Myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog); translocated to, 4	MLLT4	26.47
M26683	Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)	SCYA2	24.92
M31210	Endothelial differentiation, sphingolipid G-protein-coupled receptor, 1	EDG1	11.07
Z22555	CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1	CD36L1	4.33
M16336	CD2 antigen (p50), sheep red blood cell receptor	CD2	3.07
U43901	Laminin receptor 1 (67 kD, ribosomal protein SA)	LAMR1	2.55
M85289	Heparan sulfate proteoglycan 2 (perlecan)	HSPG2	2.11
Y13323	ADAM-like, decysin 1	ADAMDEC1	-2.03
AL133035	Filamin-binding LIM protein-1	FBLP1	-2.10
NM_002292	Laminin, beta 2 (laminin S)	LAMB2	-2.10
AL161972	Intercellular adhesion molecule 2	ICAM2	-2.12
BE301622	Pinin, desmosome associated protein	PNN	-2.17
AF053944	AE-binding protein 1	AEBP1	-2.21
Y10931	Symplekin	SYMPK	-2.24
M98457	CD209 antigen	CD209	-2.25
D87469	Cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog	CELSR2	-2.26
AW843882	Leucine-rich repeats and immunoglobulin-like domains 3	LRIG3	-2.26
X78947	Connective tissue growth factor	CTGF	-2.33
AF135021	Catenin (cadherin-associated protein), alpha-like 1	CTNNAL1	-2.36
AL136139	Enhancer of filamentation 1 (cas-like docking; Crk-associated substrate related)	HEF1	-2.36
AF055084	Monogenic, audiogenic seizure susceptibility 1 homolog (mouse)	MASS1	-2.37
AF039747	Cadherin 10, type 2 (T2-cadherin)	CDH10	-2.39
U58516	Milk fat globule-EGF factor 8 protein	MFGE8	-2.41
D49742	Hyaluronan-binding protein 2	HABP2	-2.42
U79716	Reelin	RELN	-2.42
Y11307	Cysteine-rich, angiogenic inducer, 61	CYR61	-2.45
AB007865	Fibronectin leucine rich transmembrane protein 2	FLRT2	-2.48
NM_006149	Lectin, galactoside-binding, soluble, 4 (galectin 4)	LGALS4	-2.52
M59807	Natural killer cell transcript 4	NK4	-2.59
AJ009985	Annexin A9	ANXA9	-2.61
NM_014000	Vinculin	VCL	-2.63
AL117505	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2	ATP2A2	-2.64
AF062075	Leupaxin	LPXN	-2.67
X05610	Collagen, type IV, alpha 2	COL4A2	-2.70
D12676	CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II)	CD36L2	-2.72
D21255	Cadherin 11, type 2, OB-cadherin (osteoblast)	CDH11	-2.77
U70136	Proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein)	PRG4	-2.77
S53911	CD34 antigen	CD34	-2.82
AW025274	Kin of IRRE like 2 (Drosophila)	KIRREL2	-2.85
M73255	Vascular cell adhesion molecule 1	VCAM1	-2.86
L25286	Collagen, type XV, alpha 1	COL15A1	-2.87
AL117604	Deleted in liver cancer 1	DLC1	-2.91
AJ132445	Claudin 14	CLDN14	-2.95
AL133035	Filamin binding LIM protein 1	FBLP1	-3.14
Z24725	Mitogen inducible 2	MIG2	-3.15
X15880	Collagen, type VI, alpha 1	COL6A1	-3.53
AL137480	Formin binding protein 4	FNBP4	-3.58
AF052124	Secreted phosphoprotein 1 (osteopontin, bone sialoproteinI, early T-lymphocyte activation 1)	SPP1	-3.60
AB018269	Calsyntenin 3	CLSTN3	-3.70
AB002298	PDZ domain containing 3	PDZK3	-3.97
X87838	Catenin (cadherin-associated protein), beta 1 (88 kDa)	CTNNB1	-4.64