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©2008 The WJG Press and Baishideng.
World J Gastroenterol. Dec 21, 2008; 14(47): 7199-7207
Published online Dec 21, 2008. doi: 10.3748/wjg.14.7199
Published online Dec 21, 2008. doi: 10.3748/wjg.14.7199
Table 1 Pilot-experiment data
| Probe concentration (ng/μL) | Digest time (min) | Incubation time (h)/temperature (°C) | Chromogenic time (min) | ||
| Tissue array | Tp53 | 10 | 30 | 44/48 | 110 |
| Tissue array | C-myc | 10 | 20 | 41.5/42 | 30 |
| Tissue array | CCND1 | 10 | 20 | 36/45 | 50 |
Table 2 Abnormal expression of Tp53 mRNA and over-expression of Tp53 gene in tissue array
| Histological grade | n | Tp53 positive | P value |
| Esophagus | |||
| Paracancerous tissue | 30 | 6 | P = 0.000 |
| I | 30 | 20 | |
| II | 30 | 19 | |
| III | 30 | 5 | |
| Stomach | |||
| Paracancerous tissue | 30 | 15 | P = 0.000 |
| I | 30 | 20 | |
| II | 30 | 29 | |
| III | 30 | 15 | |
| Rectum | |||
| Paracancerous tissue | 20 | 2 | P = 0.001 |
| I | 20 | 5 | |
| II | 20 | 13 | |
| III | 20 | 5 | |
| Thyroid gland | |||
| Paracancerous tissue | 20 | 2 | P = 0.023 |
| Follicular adenoma | 20 | 7 | |
| Papillary carcinoma | 20 | 10 | |
| Hepar | |||
| Paracancerous tissue | 20 | 6 | P = 0.000 |
| I | 20 | 19 | |
| II | 20 | 16 | |
| III | 20 | 15 | |
| Colon | |||
| Paracancerous tissue | 20 | 13 | P = 0.555 |
| I | 20 | 11 | |
| II | 20 | 14 | |
| III | 20 | 10 | |
| Mammary gland | |||
| Paracancerous tissue | 20 | 3 | P = 0.000 |
| Lobular hyperplasia | 20 | 4 | |
| Fibroadenoma | 20 | 4 | |
| Lobular carcinoma | 20 | 15 | |
| DCIS | 20 | 15 |
Table 3 Abnormal expression of CCND1 mRNA and over-expression of CCND1 gene in tissue array
| Histological grade | n | CCND1 positive | P value |
| Esophagus | |||
| Paracancerous tissue | 30 | 19 | |
| I | 30 | 25 | P = 0.058 |
| II | 30 | 27 | |
| III | 30 | 25 | |
| Stomach | |||
| Paracancerous tissue | 30 | 15 | |
| I | 30 | 16 | P = 0.034 |
| II | 30 | 25 | |
| III | 30 | 19 | |
| Rectum | |||
| Paracancerous tissue | 20 | 1 | |
| I | 20 | 5 | P = 0.000 |
| II | 20 | 15 | |
| III | 20 | 10 | |
| Thyroid gland | |||
| Paracancerous tissue | 20 | 3 | |
| Follicular adenoma | 20 | 10 | P = 0.000 |
| Papillary carcinoma | 20 | 16 | |
| Hepar | |||
| Paracancerous tissue | 20 | 11 | |
| I | 20 | 19 | P = 0.037 |
| II | 20 | 15 | |
| III | 20 | 14 | |
| Colon | |||
| Paracancerous tissue | 20 | 13 | |
| I | 20 | 19 | P = 0.064 |
| II | 20 | 14 | |
| III | 20 | 12 | |
| Mammary gland | |||
| Paracancerous tissue | 20 | 3 | |
| Lobular hyperplasia | 20 | 4 | P = 0.001 |
| Fibroadenoma | 20 | 8 | |
| Lobular carcinoma | 20 | 10 | |
| DCIS | 20 | 15 |
Table 4 Abnormal expression of C-myc mRNA and over-expression of C-myc gene in tissue array
| Histological grade | n | C-myc positive | P value |
| Esophagus | |||
| Paracancerous tissue | 30 | 17 | |
| I | 30 | 29 | P = 0.000 |
| II | 30 | 27 | |
| III | 30 | 20 | |
| Stomach | |||
| Paracancerous tissue | 30 | 28 | |
| I | 30 | 15 | P = 0.000 |
| II | 30 | 28 | |
| III | 30 | 25 | |
| Rectum | |||
| Paracancerous tissue | 20 | 16 | |
| I | 20 | 11 | P = 0.214 |
| II | 20 | 10 | |
| III | 20 | 13 | |
| Thyroid gland | |||
| Paracancerous tissue | 20 | 9 | |
| Follicular adenoma | 20 | 18 | P = 0.004 |
| Papillary carcinoma | 20 | 16 | |
| Hepar | |||
| Paracancerous tissue | 20 | 10 | |
| I | 20 | 11 | P = 0.813 |
| II | 20 | 10 | |
| III | 20 | 8 | |
| Colon | |||
| Paracancerous tissue | 20 | 10 | |
| I | 20 | 15 | P = 0.305 |
| II | 20 | 14 | |
| III | 20 | 11 | |
| Mammary gland | |||
| Paracancerous tissue | 20 | 7 | |
| Lobular hyperplasia | 20 | 12 | P = 0.133 |
| Fibroadenoma | 20 | 12 | |
| Lobular carcinoma | 20 | 10 | |
| DCIS | 20 | 15 |
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Citation: Liu GY, Luo Q, Xiong B, Pan C, Yin P, Liao HF, Zhuang WC, Gao HZ. Tissue array for
Tp53 ,C-myc ,CCND1 gene over-expression in different tumors. World J Gastroenterol 2008; 14(47): 7199-7207 - URL: https://www.wjgnet.com/1007-9327/full/v14/i47/7199.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.7199