Acute and persisting Th2-like immune response after fractionated colorectal &gamma;-irradiation

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Dec 14, 2008 (publication date) through Jan 21, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Colorectal Cancer

World J Gastroenterol. Dec 14, 2008; 14(46): 7075-7085
Published online Dec 14, 2008. doi: 10.3748/wjg.14.7075

Table 1 Specifications of the primer sets used for gene expression analysis by RT-PCR

Gene	Forward primer	Reverse primer
IL-1β	CAACAAAAATGCCTCGTGC	TGCTGATGTACCAGTTGGG
TNF-α	CATCTTCTCAAAATTCGAGTGACAA	TGGGAGTAGACAAGGTACAACCC
MCP-1	CAGCCAGATGCAGTTAATGCC	AGCCGACTCATTGGGATCAT
INOS	GATTTTTCACGACACCCT	GGTCCTCTGGTCAAACTC
IL-10	GTTGCCAAGCCT-TGTCAGAAA	TTTCTGGGCCATGGTTCTCT
IFN-γ	CACGCCGCGTCTTGGT	TCTAGGCTTTCAATGAGTGTGCC
Suppressors of cytokine signalling proteins
SOCS3	CCTCCAGCATCTTTGTC-GGAAGAC	TACTGGTCCAGGAACTCCCGAATG
Transcription factors
T-Bet	TCCTGTCTCCAGCCGTTTCT	CGCTCACTGCTCGGAACTCT
GATA-3	GGCGGCGAGATGGTACTG	TCTGCCCATTCATTTTATGGTAGA
Chemokine receptors
CXCR3	AGGTCAGTGAACGTCAAGTGCTAG	CAAAAAGAGGAGGCTGTAGAGGA
CCR4	GCCTCCAAGACAGACTTCCTTG	AGCGTTCGGTTCTAGTTTCCAC
Housekeeping
HPRT	GCTCGAGATGTCATGAAGGAGA	TCAGCGCTTTAATGTAATCCAGC

Citation: Grémy O, Benderitter M, Linard C. Acute and persisting Th2-like immune response after fractionated colorectal γ-irradiation. World J Gastroenterol 2008; 14(46): 7075-7085
URL: https://www.wjgnet.com/1007-9327/full/v14/i46/7075.htm
DOI: https://dx.doi.org/10.3748/wjg.14.7075