Gli-1 siRNA induced apoptosis in Huh7 cells

Table 1 Combined effect of Gli-1 siRNA and 5-Fu on apoptosis in Huh7 cells

Group	Percentage of sub G1 population (%)
Control	2.63 ± 0.28
Negative control	2.78 ± 0.39
Gli-1 siRNA	15.8 ± 2.13a
5-Fu	27.29 ± 3.14a
5-Fu + Gli-1 siRNA	44.25 ± 4.59a,c

Huh7 Cells were transfected with Gli-1 siRNA. After 6 h, cells were treated in the presence or absence of 25 &mgr;mol/L 5-Fu for 48 h. After treatment, the nuclei were stained with propidium iodide and analyzed by flow cytometry. The percentage of cells in the sub-G1 apoptotic fraction is shown in table. The data shown as mean ± SD were from three independent experiments. ANOVA analysis showed difference between groups was significant (^aP < 0.05 vs control or negative control; ^cP < 0.05 vs other treatment groups).