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©2008 The WJG Press and Baishideng.
World J Gastroenterol. Oct 21, 2008; 14(39): 6072-6077
Published online Oct 21, 2008. doi: 10.3748/wjg.14.6072
Published online Oct 21, 2008. doi: 10.3748/wjg.14.6072
Table 1 Clincal data of patients with hepatocellular carcinoma
Case | Sex | Age | Hepatitis virus | Differentiated grade | TNM score |
1 | M | 54 | HBV | WD | T1N0M0 |
2 | M | 60 | HCV | WD | T1N0M0 |
3 | F | 61 | HBV | WD | T2N0M0 |
4 | M | 62 | HBV | WD | T2N0M0 |
5 | M | 58 | HBV | WD | T1N0M0 |
6 | F | 56 | HCV | MD | T3N0M0 |
7 | F | 44 | HBV | WD | T2N0M0 |
8 | M | 49 | HCV | WD | T1N0M0 |
9 | M | 58 | HBV | WD | T2N0M0 |
10 | M | 67 | HCV | PD | T3N0M0 |
11 | M | 69 | HBV | WD | T2N0M0 |
12 | F | 63 | HCV | WD | T1N0M0 |
13 | M | 48 | HCV | MD | T2N0M0 |
14 | F | 63 | HBV | WD | T1N0M0 |
15 | M | 49 | HCV | MD | T1N0M0 |
16 | F | 51 | HBV | PD | T3N1M0 |
17 | M | 65 | HCV | MD | T3N1M0 |
18 | F | 58 | HBV | PD | T4N1M1 |
19 | M | 60 | HBV | MD | T2N1M0 |
20 | F | 56 | HCV | PD | T3N1M1 |
21 | M | 42 | HCV | PD | T3N1M0 |
22 | M | 55 | HBV | PD | T4N1M1 |
23 | M | 66 | HBV | MD | T3N1M0 |
24 | F | 70 | HCV | WD | T2N1M0 |
25 | M | 58 | HBV | PD | T4N1M1 |
26 | M | 53 | HCV | PD | T3N1M0 |
27 | M | 61 | HBV | PD | T4N1M0 |
28 | F | 65 | HBV | MD | T3N1M0 |
29 | M | 59 | HCV | MD | T3N1M1 |
30 | M | 50 | HBV | PD | T3N1M0 |
31 | F | 63 | HCV | PD | T4N1M1 |
32 | M | 66 | HCV | PD | T3N1M0 |
Table 2 Primers used to amplify cDNA at various genes in real time RT-PCR
Target gene | Objective | Forward primer sequence (5'-3') | Reverse primer sequence (3'-5') | Genebank accession no./Amplicon size |
RPS5 | qRT-PCR | GTATGCCGCCAAACGCTTC | CGCCTGTGAGCAGGTGTAT | NM_001009, 152 bp |
KRT8 | qRT-PCR | GGAGGCATCACCGCAGTTAC | GGTTGGCAATATCCTCGTACTGT | NM_002273, 637 bp |
CFLAR | qRT-PCR | GACAGAGCTTCTTCGAGACAC | GCTCGGGCATACAGGCAAAT | AF009616, 116 bp |
ATP5F1 | qRT-PCR | ACTGGGCTTATCTTGTACGCT | GCAAAGTCTGCAACAAAGGGA | NM_001688, 131 bp |
IGFBP2 | qRT-PCR | GACAATGGCGATGACCACTCA | GCTCCTTCATACCCGACTTGA | NM_000597, 121 bp |
MAP3K5 | qRT-PCR | AAAAAGGCATTTGAATCTGAGCC | GCTTGAATGACTCTCATGTGGTC | NM_005923, 233 bp |
MMP9 | qRT-PCR | GGGACGCAGACATCGTCATC | TCGTCATCGTCGAAATGGGC | NM_004994, 139 bp |
VIM | qRT-PCR | AGTCCACTGAGTACCGGAGAC | CATTTCACGCATCTGGCGTTC | AK093924, 98 bp |
ACTB | qRT-PCR | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT | NM_001101, 250 bp |
GAPD | qRT-PCR | CAACTGGTCGTGGACAACCAT | GCACGGACACTCACAATGTTC | AC002389, 260 bp |
CD58 | qRT-PCR | CTCATGGGATTGTCCTATGGAGC | GCTTGGGATACAGGTTGTCAAA | NM_001779, 154 bp |
Table 3 The 11 genes for which expression levels differed between HBV- and HCV-associated HCC
Gene name | Symbol1 | Accession2 | Fold change3 | HBV:HCV4 |
7 genes up-regulated in HBV-associated HCC | ||||
Ribosomal protein S5 | RPS5 | NM_001009 | 6.35 | 2.38 |
Keratin 8 | KRT8 | NM_002273 | 5.68 | 3.19 |
CASP8 and FADD-like apoptosis regulator | CFLAR | Y14039 | 2.86 | 2.09 |
ATP synthase, H+transporting, mitochondrial F0 complex, subunit b, isoform 1 | ATP5F1 | X60221 | 4.11 | 3.52 |
Insulin-like growth factor binding protein 2 (36 kDa) | IGFBP2 | NM_000597 | 3.37 | 2.49 |
Mitogen-activated protein kinase kinase kinase 5 | MAP3K5 | NM_005923 | 3.76 | 2.33 |
Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) | MMP9 | NM_004994 | 7.43 | 3.74 |
4 genes up-regulated in HCV-associated HCC | ||||
Vimentin | VIM | NM_003380 | 8.61 | 0.28 |
Actin-β | ACTB | X00351 | 4.13 | 0.37 |
Glyceraldehyde-3-phosphate_dehydrogenase | GAPD | NM_002046 | 5.27 | 0.29 |
CD58 antigen, (lymphocyte function-associated antigen 3) | CD58 | NM_001779 | 4.68 | 0.31 |
- Citation: Lee CF, Ling ZQ, Zhao T, Lee KR. Distinct expression patterns in hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma. World J Gastroenterol 2008; 14(39): 6072-6077
- URL: https://www.wjgnet.com/1007-9327/full/v14/i39/6072.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.6072