Gene profiles between non-invasive and invasive colon cancer using laser microdissection and polypeptide analysis

Table 1 Baseline clinical and pathological characteristics of the patients

Characteristic	Non-invasive tumor (n)	Invasive tumor (n)
Gender
Male	19	19
Female	11	21
Age
Median yr	57.9 ± 3.24	58.7 ± 3.46
Ranger yr	45-80	43-80
Cancer TNM stage
IA	30	0
III-IV	0	40
Histological type
Differentiated	16	22
Undifferentiated	14	18

Table 2 Representative list of differentially expressed genes detected by MALDI-TOF

GeneBank	Description	Cy3:Cy52
NM_006500	MCAM (melanoma adhesion molecule)	10.791
NM_002545	OPCML (opioid binding protein/cell adhesion molecule-like)	10.561
NM_002933	RNASE1 (ribonuclease, RNase A family, 1)	6.81
NM_001993	F3 (coagulation factor III)	6.291
NM_005433	YES1 (v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1)	5.9161
NM_016525	UBAP (ubiquitin associated protein)	5.876
NM_001428	ENO1 (enolase 1)	5.6921
S68616	SLC9A1 (Na+/H+ exchanger NHE-1 isoform)	5.484
NM_003254	TIMP1(tissue inhibitor of metalloproteinase 1)	5.291
NM_005903	MADH5 (mothers against decapentaplegic, homolog 5)	5.083
U82828	ATM (ataxia telangiectasia)	0.2051
NM_006343	MERTK (c-mer proto-oncogene tyrosine kinase)	0.19
NM_002985	SCYA5 (small inducible cytokineA5)	0.191
NM_005348	HSPCA (heat shock 90 kDa protein 1, alpha)	0.164
NM_003968	UBE1C (ubiquitin-activating enzyme E1C)	0.161
NM_004374	COX6C (cytochrome c oxidase subunit VIc)	0.131
NM_002990	SCYA22 (small inducible cytokine subfamily A, member22)	0.129
NM_005781	ACK1 (activated p21cdc42Hs kinase)	0.1281
NM_005139	ANXA3 (annexin A3)	0.124
AF053630	SERPINB1 (serine proteinase inhibitor, clade B, member 1)	0.123
NM_012090	MACF1 (microtubule-actin crosslinking factor 1)	0.0981
XM_042551	CAMK2A (calcium/calmodulin-dependent protein kinase)	0.0645
NM_000909	NPY1R (neuropeptide Y receptor Y1)	0.02521
NM_015230	CENTD1 (centaurin, delta 1)	0.024
NM_004958	FRAP1 (FK506 binding protein 12-rapamycin associated protein 1)	0.0205

¹Confirmed by semiquantitative RT-PCR in another group of 15 cases;

²aRNA from primary colon carcinoma tissues and the corresponding metastatic lymph node were labeled with Cy3-dCTP and Cy5-dCTP, respectively.