Copyright
©2008 The WJG Press and Baishideng.
World J Gastroenterol. Aug 28, 2008; 14(32): 5025-5031
Published online Aug 28, 2008. doi: 10.3748/wjg.14.5025
Published online Aug 28, 2008. doi: 10.3748/wjg.14.5025
Parameters | n |
Age (yr; mean ± SD, range) | 59 ± 11 (33-78) |
Male | 10 |
Female | 8 |
Primary tumor | |
Colorectal cancer | 12 |
Benign liver lesions | 2 |
Renal carcinomas | 1 |
Prostate carcinoma | 1 |
Cholangiocarcinoma | 1 |
Pseudopapillary pancreas tumor | 1 |
Operation | |
Right hemi-hepatectomy | 7 |
Left hemi-hepatectomy | 4 |
Extended right hemi-hepatectomy | 2 |
Right lateral sectorectomy | 2 |
Extended left hemi-hepatectomy | 1 |
Left lateral sectorectomy | 1 |
Non-anatomic resection | 1 |
Treatment groups | Mouse group | IP injection | No. of mice |
Negative control (Hepatocyte only) | 1 | 5 x 106 hepatocytes | 3 |
Positive control 1 (HT-29 only) | 2 | 5000 HT-29 cells | 3 |
3 | 100 000 HT-29 cells | 3 | |
4 | 500 000 HT-29 cells | 3 | |
5 | 2 million HT-29 cells | 3 | |
Positive control 2 (Hepatocytes + HT-29) | 6 | 5 million hepatocytes + 5000 HT-29 | 3 |
7 | 5 million hepatocytes + 100 000 HT-29 | 3 | |
8 | 5 million hepatocytes + 500 000 HT-29 | 3 | |
9 | 5 million hepatocytes + 2 million HT-29 | 3 | |
Bead (Hepatocyte + HT-29) | 10 | 5 million hepatocytes + 5000 HT-29 | 3 |
11 | 5 million hepatocytes + 100 000 HT-29 | 3 | |
12 | 5 million hepatocytes + 500 000 HT-29 | 3 | |
13 | 5 million hepatocytes + 2 million HT-29 | 3 |
Treatment groups | IP injection | Mouse with tumour | Percentage expression (%) |
Negative control (Hepatocyte only) | 5 x 106 hepatocytes | 0/3 | 0 |
Positive control 1 (HT-29 only) | 5000 HT-29 cells | 0/3 | 0 |
100 000 HT-29 cells | 1/31 | 01 | |
500 000 HT-29 cells | 2/3 | 67 | |
2 million HT-29 cells | 3/3 | 100 | |
Positive control 2 (Hepatocytes + HT-29) | 5 million hepatocytes + 5000 HT-29 | 0/3 | 0 |
5 million hepatocytes + 100 000 HT-29 | 0/3 | 0 | |
5 million hepatocytes + 500 000 HT-29 | 3/3 | 100 | |
5 million hepatocytes + 2 million HT-29 | 2/3 | 67 | |
Bead (Hepatocyte + HT-29) | 5 million hepatocytes + 5000 HT-29 | 0/3 | 0 |
5 million hepatocytes + 100 000 HT-29 | 0/3 | 0 | |
5 million hepatocytes + 500 000 HT-29 | 0/3 | 0 | |
5 million hepatocytes + 2 million HT-29 | 0/3 | 0 |
- Citation: Gunasegaram A, Akhter J, Yao P, Johnson LA, Riodan SM, Morris DL. Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation. World J Gastroenterol 2008; 14(32): 5025-5031
- URL: https://www.wjgnet.com/1007-9327/full/v14/i32/5025.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.5025