Construction and expression of eukaryotic plasmids containing lamivudine-resistant or wild-type strains of Hepatitis B Virus genotype C

Table 1 HBsAg and HBeAg in transfected HepG2 cells determined by ELISA

Clone	HBsAg (Sample/Negative)		HBeAg (Sample/Negative)
	48 h	72 h	48 h	72 h
pcDNA3.1 (+)	0.33 ± 0.028	0.37 ± 0.094	0.39 ± 0.046	0.38 ± 0.050
pcDNA3.1 (+)-HBV/C YMDD	3.14 ± 0.069	3.47 ± 0.413	8.72 ± 0.059	8.77 ± 0.256
pcDNA3.1 (+)-HBV/C YIDD	6.77 ± 0.099	8.26 ± 0.334	2.06 ± 0.318	2.18 ± 0.028
pcDNA3.1 (+)-HBV/C YVDD	10.30 ± 0.065	10.37 ± 0.205	5.03 ± 0.132	5.30 ± 0.117

The ratio of sample/negative (S/N) ≥ 2.1 was considered as positive HBsAg and HBeAg response.

Table 2 Real-time PCR detection of HBV DNA in transfected HepG2 cells (× 10⁸ copies/mL)

Clone	48 h	72 h
pcDNA3.1 (+)1	-	-
pcDNA3.1 (+)-HBV/C YMDD	3.57 ± 0.084	3.80 ± 0.078
pcDNA3.1 (+)-HBV/C YIDD	6.85 ± 0.143	6.90 ± 0.038
pcDNA3.1 (+)-HBV/C YVDD	17.64 ± 0.240	18.55 ± 0.127

The HBV DNA levels ≥ 5 × 10² copies/mL was considered as positive HBV DNA response.

¹The results were negative.