Preparation of chitosan-polyaspartic acid-5-fluorouracil nanoparticles and its anti-carcinoma effect on tumor growth in nude mice

Table 1 The mean weight in four groups (g)

Groups	Before treatment	After treatment (d)
		3	7	10	14
NS	20.96 ± 0.82	22.35 ± 1.17	23.41 ± 1.53	24.28 ± 1.72	24.11 ± 1.73
5-Fu	22.16 ± 2.83	22.20 ± 2.86	22.58 ± 2.80	20.86 ± 3.16	18.34 ± 3.69
CTS-Pasp	20.80 ± 1.19	22.96 ± 1.34	24.45 ± 1.26	24.14 ± 1.07	24.52 ± 1.75
CTS-Pasp-5Fu	22.01 ± 2.78	23.31 ± 2.39	25.15 ± 2.15	25.46 ± 2.08	25.53 ± 1.80

Table 2 The mean tumor volume (cm²) and inhibition rate of tumor growth (IR) in four groups

Groups	Before treatment	After treatment
		3 d	7 d	IR	10 d	IR	14 d	IR
NS	0.11 ± 0.03	0.19 ± 0.05	0.33 ± 0.10		0.62 ± 0.28		0.80 ± 0.23
5-Fu	0.12 ± 0.03	0.12 ± 0.04	0.24 ± 0.11	27.76%	0.31 ± 0.19	50.27%	0.33 ± 0.20	58.69%
CTS-Pasp	0.12 ± 0.03	0.19 ± 0.04	0.32 ± 0.10	2.80%	0.54 ± 0.10	13.49%	0.76 ± 0.26	5.58%
CTS-Pasp-5Fu	0.10 ± 0.01	0.11 ±0.04	0.13 ± 0.05	60.76%	0.19 ± 0.05	68.99%	0.23 ± 0.07	70.82%

Table 3 Tumor weight (g) and tumor inhibition rate (TIR) at the end of experiment

Groups	Mean tumor weight (g)	TIR (%)
NS	1.47 ± 0.18	-
5-Fu group	0.51 ± 0.11	65.30
CTS-Pasp group	1.40 ± 0.13	5.09
CTS-Pasp-5Fu group	0.40 ± 0.13	72.79

Table 4 WBC, TB, ALT, creatinine and cell colony culture in four groups

Groups	WBC(109/L)	TB(&mgr;mol/L)	ALT(U/L)	Creatinine (&mgr;mol/L)	CFU-GM (unit/plate)
NS	1.48 ± 0.84	1.24 ± 0.19	67.44 ± 19.33	27.83 ± 6.95	247 ± 18.06
5-Fu	1.42 ± 0.50	15.08 ± 4.32	145.67 ± 42.52	22.98 ± 8.33	120 ± 8.25
CTS-Pasp	1.50 ± 0.46	1.29 ± 0.67	65.43 ± 5.95	24.37 ± 4.89	241 ± 6.26
CTS-Pasp-5Fu	1.54 ± 0.23	1.26 ± 0.49	58.84 ± 4.36	22.18 ± 6.18	239.6 ± 7.40

Table 5 Histological change in four groups

Groups	Tumor number	Score of histological tumor necrosis
		-	+	++	+++
NS	8	3	5	0	0
CTS-Pasp	8	2	6	0	0
5-Fu	6	1	4	1	0
CTS-Pasp-5Fu	8	0	4	3	1

Table 6 Expression of Bcl-2, Bax in four groups (n = 8)

Groups		Bcl-2	Bax
NS groups		39.14 ± 5.84	18.94 ± 9.92
CTS-Pasp		38.62 ± 6.53	18.26 ± 7.65
5-Fu		31.74 ± 5.49a	33.24 ± 4.89a
CTS-Pasp-5Fu		22.74 ± 4.55bc	37.22 ± 9.21d

^aP < 0.05 vs NS;

^cP < 0.05 vs 5-Fu;

^bP < 0.001 vs NS;

^dP < 0.001 vs NS group.