Primary study of leptin and human hepatocellular carcinoma in vitro

Table 1 Effect of leptin on proliferation of HepG2

Concentration (ng/mL)	A
	24 h	48 hd	72 hd
0	0.29 ± 0.01	0.34 ± 0.01	0.37 ± 0.01
50b	0.34 ± 0.02	0.42 ± 0.01	0.54 ± 0.02
100b	0.41 ± 0.03	0.53 ± 0.05	0.62 ± 0.02
200b	0.46 ± 0.04	0.63 ± 0.01	0.73 ± 0.03

^bP < 0.01 vs group of 0 ng/mL leptin;

^dP < 0.01 vs group of incubation 24 h.

Table 2 Effect of leptin on cell cycle of HepG2

Concentration (ng/mL)	Cell phase (%)
	G0-G1	S	G2-M
0	61.8 ± 2.5	36.5 ± 1.0	1.6 ± 0.3
50	56.9 ± 2.2	38.5 ± 0.9	4.7 ± 1.4
100	50.2 ± 2.6a	40.3 ± 1.2	9.4 ± 2.1a
200	43.6 ± 2.7b	42.0 ± 0.3a	14.4 ± 2.5a

^aP < 0.05,

^bP < 0.01 vs group of 0 ng/mL leptin.