Copyright
©2008 The WJG Press and Baishideng.
World J Gastroenterol. Apr 14, 2008; 14(14): 2235-2240
Published online Apr 14, 2008. doi: 10.3748/wjg.14.2235
Published online Apr 14, 2008. doi: 10.3748/wjg.14.2235
Table 1 Labeling efficacy and radiochemical purity of labeled compounds (mean ± SD)
Interval between binding and labeling (d) | Radioactivity of 99mTcO4- (MBq) | |||||
15 | 30 | 60 | 370 | 740 | 1480 | |
Labeling efficiency (%) | ||||||
Via HYNIC | 57.36 ± 3.69 | 62.13 ± 4.25 | 62.87 ± 3.04 | 58.74 ± 5.32 | 62.86 ± 4.27 | 63.28 ± 3.38 |
Via NHS-MAG3 | 67.35 ± 4.03 | 68.35 ± 3.56 | 69.85 ± 4.63 | 68.67 ± 4.82 | 70.31 ± 5.09 | 71.56 ± 5.37 |
Radiochemical purity (%) | ||||||
99mTc-HYNIC-ASON | 95.75 ± 5.21 | 96.32 ± 4.92 | 95.86 ± 5.28 | 96.56 ± 4.45 | 96.87 ± 3.65 | 97.16 ± 4.34 |
99mTc-MAG3-ASON | 96.43 ± 4.69 | 95.67 ± 5.17 | 96.39 ± 4.78 | 96.35 ± 6.12 | 95.86 ± 4.67 | 96.54 ± 5.65 |
Table 2 Radiochemical stability of labeled compounds (%, mean ± SD)
Incubation time at room temperature (h) | Incubation time at 37°C (h) | |||||
1 | 2 | 4 | 1 | 2 | 4 | |
99mTc-HYNIC-ASON | 93.43 ± 5.32 | 89.17 ± 4.62 | 87.16 ± 5.36 | 92.75 ± 4.46 | 89.52 ± 3.67 | 86.86 ± 5.49 |
99mTc-MAG3-ASON | 97.26 ± 6.02 | 96.68 ± 5.54 | 96.39 ± 4.68 | 95.86 ± 5.69 | 95.47 ± 4.07 | 94.79 ± 5.34 |
Table 3 Biodistribution of labeled compounds in BALB/c mice (% ID/g) (mean ± SD)
Tissue | 0.5 h | 1 h | 2 h | 4 h | Paired | t-test | ||||
M | H | M | H | M | H | M | H | |||
Blood | 1.12 ± 0.76 | 6.21 ± 1.03 | 2.38 ± 0.63 | 6.56 ± 1.11 | 1.14 ± 0.42 | 3.58 ± 1.21 | 1.10 ± 0.09 | 2.83 ± 0.54 | t = 4.347 | P = 0.022 |
Heart | 0.62 ± 0.31 | 2.13 ± 0.45 | 0.58 ± 0.07 | 1.64 ± 0.34 | 0.32 ± 0.05 | 1.16 ± 0.12 | 0.18 ± 0.08 | 0.81 ± 0.13 | t = 5.362 | P = 0.013 |
Lungs | 3.11 ± 0.82 | 2.68 ± 0.65 | 3.87 ± 1.36 | 3.23 ± 1.04 | 3.04 ± 0.79 | 2.78 ± 1.03 | 2.08 ± 0.62 | 1.76 ± 0.36 | t = -4.934 | P = 0.016 |
Liver | 7.52 ± 2.45 | 11.46 ± 2.31 | 13.19 ± 1.47 | 15.24 ± 2.53 | 9.21 ± 1.03 | 12.89 ± 1.68 | 9.48 ± 2.56 | 10.46 ± 1.97 | t = 3.806 | P = 0.032 |
Kidneys | 11.42 ± 3.34 | 4.17 ± 1.05 | 17.13 ± 2.86 | 5.03 ± 0.94 | 24.58 ± 3.57 | 2.78 ± 0.68 | 21.95 ± 4.02 | 2.28 ± 0.95 | t = -4.511 | P = 0.020 |
Spleen | 2.71 ± 1.62 | 4.87 ± 2.36 | 5.65 ± 0.93 | 6.08 ± 1.93 | 5.35 ± 0.26 | 4.08 ± 1.54 | 4.84 ± 1.33 | 5.21 ± 2.04 | t = 0.603 | P = 0.589 |
Stomach | 1.08 ± 0.86 | 7.46 ± 2.13 | 2.58 ± 0.95 | 11.48 ± 3.01 | 1.73 ± 0.21 | 7.49 ± 1.86 | 1.41 ± 0.34 | 7.85 ± 3.02 | t = 9.901 | P = 0.002 |
Intestines | 0.53 ± 0.31 | 1.26 ± 0.31 | 0.86 ± 0.14 | 2.68 ± 0.95 | 1.23 ± 0.19 | 6.44 ± 2.13 | 1.96 ± 0.53 | 7.84 ± 2.34 | t = 2.706 | P = 0.073 |
Muscle | 1.35 ± 0.16 | 0.54 ± 0.21 | 0.87 ± 0.63 | 0.94 ± 0.81 | 0.75 ± 0.08 | 0.42 ± 0.06 | 0.64 ± 0.15 | 0.19 ± 0.05 | t = -2.095 | P = 0.127 |
Table 4 Human colon carcinoma HT29 cellular uptake of 99mTc-HYNIC-ASON and 99mTc-MAG3-ASON (mean ± SD)
10 min | 20 min | 40 min | 60 min | 120 min | |
99mTc-HYNIC-ASON (%) | 0.43 ± 0.08 | 0.56 ± 0.21 | 0.93 ± 0.54 | 1.42 ± 0.64 | 1.67 ± 0.86 |
99mTc-MAG3-ASON (%) | 2.78 ± 0.81 | 5.64 ± 0.51 | 7.82 ± 2.53 | 13.63 ± 2.71 | 15.25 ± 3.13 |
- Citation: Li YC, Tan TZ, Zheng JG, Zhang C. Anti-sense oligonucleotide labeled with technetium-99m using hydrazinonictinamide derivative and N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline: A comparison of radiochemical behaviors and biological properties. World J Gastroenterol 2008; 14(14): 2235-2240
- URL: https://www.wjgnet.com/1007-9327/full/v14/i14/2235.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.2235