Copyright
©2008 The WJG Press and Baishideng.
World J Gastroenterol. Mar 28, 2008; 14(12): 1851-1857
Published online Mar 28, 2008. doi: 10.3748/wjg.14.1851
Published online Mar 28, 2008. doi: 10.3748/wjg.14.1851
Table 1 Primers for the target genes and β-actin used in real-time PCR
| Target gene | Primer | Target fragment length (bp) |
| β-actin | 5’-TGACGAGGCCCAGAGCAAGA-3’(F) | 331 |
| 5’-ATGGGCACAGTGTGGGTGAC-3’(R) | ||
| TNF-α | 5’-GGCAGCCTTGTCCCTTGAAGAG-3’(F) | 171 |
| 5’-GTAGCCCACGTCGTAGCAAACC-3’(R) | ||
| IL-6 | 5’-CCACTTCACAAGTCGGAGGCTTA-3’(F) | 108 |
| 5’-GTGCATCATCGCTGTTCATACAATC-3’(R) | ||
| TLR4 | 5’-CTCACAACTTCAGTGGCTGGATTTA -3’(F) | 178 |
| 5’-TGTCTCCACAGCCACCAGATTC-3’(R) | ||
| TLR2 | 5’-GGCCACAGGACTCAAGAGCA-3’(F) | 102 |
| 5’-AGAGGCCTATCACAGCCATCAAG-3'(R) | ||
| CD14 | 5’- GAATCCCAGTCGGAGGCGTA-3’(F) | 94 |
| 5’-GGAGCAAAGCCAAAGTTCCTGA-3’(R) |
- Citation: Peng JH, Hu YY, Cheng Y, Han C, Xu LL, Feng Q, Chen SD, Tao Q, Li HS, Li XM. Effect of JIANPI HUOXUE decoction on inflammatory cytokine secretion pathway in rat liver with lipopolysaccharide challenge. World J Gastroenterol 2008; 14(12): 1851-1857
- URL: https://www.wjgnet.com/1007-9327/full/v14/i12/1851.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.1851