Comparative genomic hybridization analysis of genetic aberrations associated with development of esophageal squamous cell carcinoma in Henan, China

Table 1 CGH results of 37 ESCC cases, Linzhou, Henan

Case No.	Gains	Losses
66 908	5p, 10q11-10q21	3p21-3p11, 9,10q23-10qter, 15
66 865	1p31-1qter, 3q, 3q22-3qter, 5p, 6q15-6qter, 7p14-7qter, 8q13-qter, 18p	4pter-4p13, 9q, 5q11-14, 11p, 18q12-18qter
66 867	3q, 5pter-5q12, 6p12-6q12, 7p12-7qter, 8q, 16	3pter-3p13, 4q23-4qter, 8p, 9,11p13-11qter, 13,17p, Xp
66 909	3q, 5p, 7,8q12-8qter,	9p13-9qter, 19
66 755	1q, 2p, 3q, 8q, 9p, 11q13-11q21	1pter-1p31, 3pter-3p13, 8p, 11q22-11qter, 15,16,17,19,21,22
16 182	4p13-4qter, 8p12-8qter, 9p, 16p11-16qter, 16p11-16q21, 18p	1pter-1p32, 3p, 4pter-p14, 11q22-11qter, 18q, 19
66 907	2q14.1-14.3, 2q31-32, 3q23-3qter, 5p, 6q, 7p, 8,12p, 18p	3p, 4,5q, 10,11q21-qter, 12q, 17p, Xp
66 912	2q14-2qter, 3q22-3qter, 5p, 6p12-6q15, 8q, 12q11-12q22, 13,20q	1pter-1p31, 5q, 11pter-11q12, 16,17p, 19
66 910	6p21-6q13, 7q11-7q31, 8q, 13	1pter-1p31, 8p, 16q
66 948	2p, 3q, 3q22-3qter, 7,8q, 10p, 14,16q11-12	1pter-1p31, 3p, 6q, 8p, 11q14-11qter, 13,19,21
16 146	3q, 5pter-5q12, 6p21-6q12, 7q21-7q22, 8q, 17q, 18pter-18q11	8p, 9q, 16,18q12-18qter
16 634	3p12-3qter, 5pter-5q12, 7q21-7qter, 8q, 12p, 18p, 20,20p	1pter-1p33, 4pter-4p15, 9,11pter-11q12, 13,16q, 17,18q, 19p, 21,22
16 179	1q, 3q, 3q22-3qter, 4p13-4q21, 5p, 6p, 7, 8q, 13	1pter-1p31, 3p, 5q, 8p, 9,10,14,19,Y
16 601	5p, 8p12-8qter,Y	3p, 19
16 605	6p, 8q, 11q11-11q21, 14,16pter-16q22, 17p, 20p11-20q13, 22	11q22-11qter, 18q12-18qter
16 609	1q, 2,3q, 3q22-3qter, 5p, 8q, 11p13-11q21, 12p, 13q21-13qter, 16q12-16qter	1p, 3p, 4,5q, 8p, 9p, 10,12q, 18p11-18qter, 21, Xp
16 610	3q, 7p, 8q, 12p12-12q23, 16p11-16q21, 18	1pter-1p31, 8pter-8p12, 11,17,19,Y
16 615	1q31-1qter, 2pter-2q33, 3q13-3qter, 4q12-4q21, 5p, 7q21-7q31, 8q22-8qter, 12,18p	2q34-2qter, 5q13-5q14, 7p11-7q11, 8p, 9,22, X
16 616	6p21-6qter, 7pter-7q21, 8p12-8qter, 10,14	7q22-7qter
16 629	1p32-1p21, 2q11-2q32, 3q22-3qter, 6p, 8q, 9p, 11p, 12q14-12q22, 18,20	2q33-2qter, 6q, 8p, 9q, 10q, 11q, 13,16, X
16 639	1p31-1p13, 3q13-3qter, 11q13-q23, 12pter-12q13, 14	3pter-13, 4pter-12, 5q21-qter, 21,22, Xpter-q13
16 658	3q, 4pter-4q13, 5p, 6p12-6q14, 8q	3p, 8p, 9q21-9qter, 11,19
16 721	1p11-1q22, 7pter-7q31, 14q21-14q23, 19,20	2p, 3p, 5q12-5qter, 6q, 11p, Y
16 740	5p13-5q13, 18pter-18q12	6pter-6p21, 14q13-14qter
19 110	1q21-1qter, 2q, 6,7p, 11p, 11q13-q22, 14, 16p13-16p11	1pter-1p34, 8pter-8q12, 8q23-8qter, 15q22-15qter, 16q, 17, 22
19 315	6p12-6q14, 13,16q11-16q21, 18pter-18q12	1p13-22, 3pter-q21, 8pter-12, 18q21-qter, X
19 419	2q24-2q32, 3q, 8,13,20	3p, 9p22-9q21, 10,16p, 17
16 172	8q22-8qter	13
16 181	3q13-3qter, 7,8,12,17,18p, 20,22	2q14-2q34, 3p, 4q, 5q11-q23, 6q11-24, 13
16 186	3p12-3qter, 5p, 6p21-6q15, 7, 8q, 11p14-11q14, 16,17pter-17q12, 20pter-20q11	13,18q
16 604	1p31-1qter, 3q, 4p12-4q12, 6, 7,8p22-8qter, 10pter-10q11, 11q12-11q23, 14q11-14q31	2pter-2q22, 3p, 5q21-5qter, 9p12-9qter, 13,15,17,18
16 624	1pter-1p31, 3pter-3p21, 3q, 5p, 6p21-6p12, 7p, 8q, 11,18pter-18q12, 19p13-19qter, 20	2p, 3p21-3p11, 4,5q, 8p, 10,14,16q, Y
16 633	8q22-23, 9,12p11-12q21, 21,X	3p, 6,10,11p, 12q21-12qter, 16q23-16qter, 18q,
16 632	1q, 2pter-q32, 3p12-qter, 5pter-q12, 8p22-qter, 9q, 11, 12pter-q13, 15, 16p12-q21, 17p12-q24, 18pter-q12	3pter-3p13, 4,10,13
16 624	3q, 3q22-3qter, 5p, 11p11-11q13	3pter-3p12, 11q14-11qter, 13,18, X
16 625	1q, 3q, 4p14-4q21, 4q26-4qter, 19p11-19q13	1pter-1p319q33-9qter, 15,16,17p
66 945	1p31-1qter, 3q, 5p, 6q14-6qter, 8q12-8qter, 9pter-9q31	3p, 4,5q, 9q31-9qter, 11,13,14

Table 2 CGH results of lymph node metastasis, Linzhou, Henan

Case No.	Gains	Losses
16 148	2p12-2q32, 3p12-3qter, 5p, 9q	3pter-3p13, 5q31-5qter,X
L16 148	1q, 2q11-2q31, 3q, 5p, 9p12-9qter, 12,20	2q32-2qter, 3p, 4,5q, 6pter-6p22, 19,X
16 668	3q13-3qter, 4q31-4qter, 7pter-1p11, 8q12-8qter, 18p	1p, 4pter-4p14, 4q21-4q28, 7q22-7qter, 9p
L16 668	3q, 4p12-4q13, 7p15-7q22, 8p21-8qter, 13,15pter-15q15, 18pter-18q12, 20pter-20q12	3p, 4pter-4p13, 4q13-4q25, 7q31-7qter, 9p, 11q13-11qter
16 669	3p12-3qter, 7p, 8q, 12pter-12q13, 14	1p, 3pter-3p12, 4p, 10,11,17,19
L16 669	3p12-3qter, 6p12-6q14, 7p, 8p12-8qter, 14,20p11-20qter	1p, 3pter-3p12, 4p, 10,11,19
16v688	1q21-1qter, 3q, 5p, 6p12-6q24, 7q11-31, 8p22-8q23, 11q, 11q11-11q14, 18pter-18q12	3p, 4q13-4qter, 6pter-6p21, 7q32-7qter, 9p12-9qter, 10p, 11p, 16q, 17pter-17p11, 17q22-17qter, 19
L16 688	1p13-1qter, 1p13-1q21, 2,3q, 6p21-6q12, 8q11-8q23, 11q, 11q11-11q14, 12p, 18pter-18q11, 18p11-18q11, 20	3p, 4,5pter-5q15, 6pter-6p21, 6q16-6qter, 7p, 7q31-7qter, 8pter-8p12, 9q, 10p, 11p, 13,17p, 17q22-17qter, 19,21
16 691	1q, 2pter-2q21, 3q, 11q11-11q13, 13q14-13qter, 16q11-16q13, 17q21.1-21.3, 20	3p, 8q11-8q22, 9q, 11q14-11qter, 13pter-13q14, 16p, 19,21,22
L16 691	1q, 2pter-2q21, 3q, 11q11-11q13, 13q14.3-13qter, 16q11-16q13, 17q21.1-17q21.3, 20	1pter-1p32, 3p, 8q11-8q22, 9q, 11q14-11qter, 13pter-13q14.2, 16p, 17p, 19,21,22
16 670	3q, 8q, 12p, 14,16p11-16q21, 18p, 20	1pter-1p31, 9p12-9qter, 17,18q, 19,Y
L16 670	3q, 8q, 12p, 14,18p, 20	1pter-1p34, 3pter-3p13, 9q, 10,11p11-11q12, 17,19p,Y
16 710	1p33-1qter, 2pter-2q32, 3p13-3qter, 5pter-5q12, 6q16-6qter, 7p, 11q11-11q23, 12pter-12q15, 13,16p13-16qter, 17p,	4p, 18pter-18q21
L16 710	1q, 2p22-12, 2q22-2q32, 3q, 5p, 6q21-6qter, 7p, 9pter-9p21, 11q11-11q14, 13,16q, 17pter-17q12	1p, 4,6pter-6q16, 8pter-8p12, 10pter-10p12, 10q23-10qter, 11p, 11q14-11qter, 12q, 16p, 18,21
16 727	1p13-1q24, 2p24-2p23, 2p14-2p12, 3q13-3qter, 5p, 7q11-7qter, 8q, 12pter-12q12, 14q23-14qter, 18pter-18q12, 20	1p, 2q32-2qter, 3pter-3p21, 4,5q, 8p, 11p, 18q12-qter
L16 727	1q, 2p14-2p11, 3q13-3qter, 5p, 7q11-7qter, 8q, 12pter-12q13, 14,17q, 18p, 20p	1p, 2q32-2qter, 3p, 4,5q, 9p, 10p, 11p, 15pter-15q22, 18q12-18qter, 21
16 728	3q, 8p12-8qter, 11p11-11q14, 12pter-12q12, 16p, 18pter-18q12, 20	3p,Xp
L16 728	1p21-1pter, 3q, 3q24-3qter, 7p12-7q22, 8q, 8q21-8qter, 12p12-12q12, 14q11-14q21, 20p	4q, 8pter-8p22, 10,11,13q12-13q31, 14q23-14qter, 20q,Xp
16 730	1q, 3q, 6,7,8,12pter-12q13, 20q12-20qter	3p, 9p, 16q, 19p13-19q13
L16 730	1q, 2,3q, 6,6p21-6q13, 12pter-12q14, 18p11-18q12	1p, 3p, 4q26-4qter, 5q, 9,10,13,16,18q21-18qter, 19p, 21
16 731	3q, 4p13-4q13, 16q11-16q12, 11p13-11q13, 12	9,13
L16 731	3q, 4p13-4q13, 8p12-8qter	3p21-3p11, 9q, 10,17,18q12-18qter, 19
16 738	1q, 3p13-3qter, 5p, 8q	1pter-1p31, 3pter-3p14, 4,11,16,17,19,Xpter-q21
L16 738	1p13-1qter, 1p13-1q21, 5pter-5q12, 6p21-6q14, 8q, 20	1pter-1q13, 2pter-2p21, 3p, 4,8p, 11pter-11p12, 11q14-11qter, 13,16p, 17,18, Xpter-q21
16 742	1q11-1q24, 3q, 3q24-3qter, 5pter-5q12, 8p11-8qter, 9q, 10p, 12p, 19p12-19q12, 20	1p, 3p, 4,6q16-6qter, 8pter-8p12, 9p, 10q22-10qter, 11,13,14q21-14qter
L16 742	1q11-1q24, 2p21-2qter, 3q, 3q22-3qter, 5p13-5q13, 7,8q, 9q21-9q32, 10p12-10q11, 12pter-12q13, 20	1qter-1q32, 3p, 4,6q, 8p, 10q21-10qter, 11,12q21-12qter, 13,14q21-14qter, 19
16 745	1q, 2q24-2q33, 3q, 5p, 6q13-6q24, 8,9p, 10pter-10q21, 12q14-12q21	1pter-1p32, 2q21-2q24, 2q34-2qter, 3p, 4,5q, 7q31-7qter, 11q14-11qter, 13pter-13q21, 16,18,19,21
L16 745	1q, 2q24-2q35, 3q, 5p, 6p21-6q25, 8	2q21-2q24, 2q34-2qter, 3p, 4,5q, 7q31-7qter, 11q14-11qter, 13pter-13q21, 16,18,19,21
16 770	3p12-3qter, 8p12-8qter	17p, 19p, 22
L16 770	3q, 6p, 16p11-16q21, 18p, 20	11,13,17p, 18q, 19p, 22

Table 3 Chromosomal aberrations with primary ESCC and lymph node metastasis

Chromosomal changes	Primary tumor (%)	Lymph node metastasis %	P value
Gain of 6p	2/15 (13)	7/15 (47)	0.05
Gain of 20p	5/15 (40)	11/15 (73)	0.03
Loss of 10p	2/15 (13)	8/15 (53)	0.05
Loss of 10q	2/15 (13)	7/15 (47)	0.05