Mutations in components of the Wnt signaling pathway in gastric cancer

Table 1 Primers used for AXIN1, AXIN2 and CTNNB1 gene amplification

Exon	Sense primer	Antisense primer
AXIN1
exon1A	5’-TGGTCGTGTTTCATGGACCC-3’	5’-AATGCAGTGACTCAGCCCACT-3’
exon1B	5’-GGATCTGGACCTGGGGTATG-3’	5’-ATAGTGGCCTGGATTTCGGT-3’
exon1C	5’-ATCATGAAGCAGCTGATCG-3’	5’-GAGGTGAGTACAGAAAGTGGAC-3’
exon2	5’-CTGTTGGCAGGCTGTCACT-3’	5’-GTCCGTGAGGGACTGGGTA-3’
exon3	5’-CTGGCCCTCCTGCTCCTCT-3’	5’-AGGACGATGGGCTGAGGAC-3’
exon4	5’-TTTAGCCTGTGACCTTTCAAC-3’	5’-ATCCCGGCGGCAAGAA-3’
exon5A	5’-AGCTGGTGCTGAGAGGTGATG-3’	5’-CCCTGACTTGGGTACGTGCTT-3’
exon5B	5’-TGGGCACGTGGCCAAGAT-3’	5’-AAGCCCCCTCCTCACTGACAG-3’
exon6	5’-CACCGGAGGCCAGGGCGACT-3’	5’-TGGCAAAGCAGGCCCCACGA-3’
exon7	5’-CCAGGGTGTCGGCCACAGTC-3’	5’-CCCCAGGAGTGGTGCTGTGGT-3’
exon8	5’-GCGCAGCAGCATTGGTCGAG-3’	5’-GGGCAGGACCGGGAGGACC-3’
exon9	5’-AGTCCAAAACCAGGTACCAC-3’	5’-AAACCCTCTTTTTCATACCG-3’
exon10	5’-CACGCCGTCCCCTGCCAC-3’	5’-GACACCCGTGCCCGCCAA-3’
AXIN2
exon7	5’-TCCTTGTTTTGCCCAAAGC-3’	5’-GGTCAGGGGAGGCATCGCAG-3’
CTNNB1
exon3
P3-4	5’-GATTTGATGGAGTTGGACATGG-3’	5’-TGTTCTTGAGTGAAGGACTGAG-3’

Table 2 The profile of AXIN1 polymorphisms

Exon	Nucleotide changes	Amino acid1	Frequency (%)
1	334 C→T	Gly74Gly	4.3
	874 C→T	Asp254Asp	42.8
5	1396 G→A	Ser428Ser	10.0
	1690 C→T	Asp526Asp	2.9
6	1942 T→G	Ala610Ala	12.9

¹Amino acid number begins from Met at position 113 of GenBank accession, No. AF009674.