Comparison of ligase detection reaction and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B

Table 1 Results of LDR and real-time PCR for detection of mixed plasmids containing YIDD and YMDD at a final concentration of 10⁶ copies/mL

YIDD plasmid in the mixture	LDR	Real-time PCR
100% (106 copies/mL)	YIDD	YIDD
50% (5 × 105 copies/mL)	YIDD/YMDD	YIDD/YMDD
10% (105 copies/mL)	YIDD/YMDD	YIDD/YMDD
1% (104 copies/mL)	YIDD/YMDD	YIDD/YMDD
0.1% (1000 copies/mL)	YIDD/YMDD	YIDD/YMDD
0.01% (100 copies/mL)	YIDD/YMDD	YMDD

Table 2 Comparison of results obtained by LDR and real-time PCR for 52 clinical samples

Types	No. of samples (%)
	LDR	Real-time PCR
YMDD	0 (0)	0 (0)
YIDD	39 (75)	39 (75)
YVDD	9 (17)	9 (17)
YIDD + YVDD	4 (8)	4 (8)