Survey of molecular profiling during human colon cancer development and progression by immunohistochemical staining on tissue microarray

Table 1 Comparison of IHC results (p53, p21, cyclin D1, bcl-2 and bax) among varying tissues

Groups	n	p53				P	p21				P	Cyclin D1				P	bcl-2				P	bax				P
		-	+	++	+++		-	+	++	+++		-	+	++	+++		-	+	++	+++		-	+	++	+++
Cancer	85	39	6	19	21		19	9	28	26		17	16	30	22		5	1	46	32		21	9	39	16
Adenomas	18	15	2	1	0	0.0032	8	5	4	1	0.0001	3	7	8	0	0.0001	1	1	7	8	0.0012	0	0	9	9	0.0001
Benign tissue	9	9	0	0	0		9	0	0	0		8	1	0	0		2	3	3	0		0	1	1	7
Total	112	63	8	20	21		36	14	32	27		28	24	38	22		8	5	56	40		21	10	49	32

¹Chi-square test;

²Fisher’s exact test.

Table 2 p53, p21, cyclin D1, bcl-2 and bax status in relation to clinicopathological parameters

Clinicopathalogicalparameters		Total	p53				P	p21				P	cyclinD1				P	bcl-2				P	bax				P
			-	+	++	+++		-	1+	2+	3+		-	1+	2+	3+		-	+	++	+++		-	+	++	+++
Age	< 58	39	17	5	8	9	0.3011	10	4	13	12	0.9661	8	6	13	12	0.7521	3	1	21	13	0.6542	11	3	17	8	0.7721
	≥ 58	46	22	1	11	12		9	5	15	14		9	10	17	10		2	0	25	19		10	6	22	8
Gender	Male	48	24	2	10	12	0.6081	12	1	17	16	0.0401	11	9	16	12	0.8911	5	1	26	16	0.1392	14	6	20	8	0.5791
	Female	37	15	4	9	9		7	8	11	10		6	7	14	10		0	0	20	16		7	3	19	8
Histological grade	Low	24	18	0	3	3		10	4	3	6		9	4	8	3		3	1	16	4		11	3	9	1
	Moderate	50	17	3	13	17	0.0052	6	3	21	18	0.0132	5	9	20	16	0.0822	2	0	24	23	0.0752	8	6	25	11	0.0442
	High	11	4	3	3	1		3	2	4	2		3	3	2	3		0	0	6	5		2	0	5	4
Lymph node metastasis	Negative	53	19	4	16	14	0.0671	9	4	19	19	0.1991	11	11	20	11	0.5751	2	0	29	21	0.4072	10	6	26	11	0.4571
	Positive	32	20	2	3	7		10	5	9	7		6	5	10	11		3	1	17	11		11	3	13	5
Distant metastasis	Negative	71	30	6	18	17	0.2922	14	8	23	23	0.6442	12	16	24	19	0.1002	3	0	39	29	0.0522	17	7	32	15	0.6942
	Positive	14	9	0	1	4		5	1	5	3		5	0	6	3		2	1	8	3		4	2	7	1
Dukes stage	A	15	5	2	6	2		3	2	5	5		2	7	4	2		0	0	9	6		2	2	8	3
	B	33	10	2	9	12	0.0612	3	2	13	13	0.2862	7	4	15	7	0.0382	1	0	18	14	0.4872	6	3	16	8	0.7162
	C	23	15	2	3	3		8	4	5	5		3	5	5	10		2	0	12	9		9	2	8	4
	D	14	9	0	1	4		5	1	5	3		5	0	6	3		2	1	8	3		4	2	7	1

¹ Chi-square test;

² Fisher’s exact test.

Table 3 Comparison of IHC results of Cox-2 and nm23-h1among varying tissues

Histology	n	Cox-2				P	nm23-h1				P
		-	+	++	+++		-	+	++	+++
Cancer	85	4	4	30	47		5	7	33	29
Adenomas	18	2	0	13	3	0.002	2	1	13	2	0.007
Benign tissue	9	3	0	1	5		4	1	2	1
Total	112	9	4	44	55		11	9	48	32

Fisher’s exact test.

Table 4 Cox-2 and nm23-h1 status in relation to clinicopathological data

Groups		n	Cox-2				P	nm23-h1				P
			-	+	++	+++		-	+	++	+++
Age (yr)	< 58	39	3	1	17	18	0.239	4	3	21	10	0.272
	≥ 58	46	1	3	13	29		1	4	22	19
Gender	Male	48	1	2	17	28	0.628	5	5	24	14	0.157
	Female	37	3	2	13	19		0	2	19	15
Histological grade	Low	24	3	3	12	6		3	6	13	2
	Moderate	50	1	1	14	33	0.008	2	0	23	24	0.000
	High	11	0	0	4	7		0	1	7	3
Lymph node metastasis	Negative	53	1	2	17	33	0.223	2	3	29	18	0.434
	Positive	32	3	2	13	14		3	4	14	11
Distant metastasis	Negative	71	1	3	27	40	0.026	3	3	39	26	0.008
	Positive	14	3	1	3	7		2	4	4	3
Dukes stage	A	15	0	1	3	11		1	1	11	2
	B	33	0	0	12	21	0.016	0	1	16	16	0.014
	C	23	1	2	12	8		2	1	12	8
	D	14	3	1	3	7		2	4	4	3

Fisher’s exact test.

Table 5 Comparison of the IHC results of β-caternin and c-myc among varying tissues

Histologicalcharacteristics	Total	β-caternin		P	c-myc		P
		-	+		-	+
Cancer	85	15	70		16	68
Adenomas	18	5	13	0.034	4	14	0.003
Benign tissue	9	5	4		6	2
Total	112	25	87		26	84

Fisher’s exact test.

Table 6 PTEN and p-Akt1 status in relation to clinicopathological data

Clinicopathologicalparameters		Total	PTEN				P	p-Akt1				P
			-	+	++	+++		-	+	++	+++
Age	< 58	39	3	2	20	14	0.8602	10	0	16	13	0.4081
	≥ 58	46	2	2	22	20		9	3	19	15
Gender	Male	48	4	4	24	16	0.1672	14	0	19	15	0.0961
	Female	37	1	0	18	18		5	3	16	13
Histological grade	Low	24	4	2	14	4		11	3	6	4
	Moderate	50	1	2	24	23	0.0292	8	0	26	16	0.0002
	High	11	0	0	4	7		0	0	3	8
Lymph node metastasis	Negative	53	3	2	22	26	0.1322	7	1	26	19	0.0321
	Positive	32	2	2	20	8		12	2	9	9
Distant metastasis	Negative	71	2	3	37	29	0.0602	13	3	31	24	0.2752
	Positive	14	3	1	5	5		6	0	4	4
Dukes stage	A	15	0	1	7	7		1	0	6	8
	B	33	1	1	15	16	0.2812	4	1	17	11	0.1062
	C	23	1	1	15	6		8	2	8	5
	D	14	3	1	5	5		6	0	4	4

¹ Chi-square test;

² Fisher’s exact test.

Table 7 β-catenin and c-myc status in relation to clinicopathological data

Clinicopathological	parameters	Total	β-caternin		P	c-myc		P
			-	+		-	+
Age	< 58	39	6	33	0.9831	9	29	0.1171
	≥ 58	46	7	39		5	41
Gender	Male	48	8	40	0.6891	10	38	0.2371
	Female	37	5	32		4	32
Histological grade	Low	24	10	14		11	13
	Moderate	50	3	47	0.0002	3	46	0.0002
	High	11	0	11		0	11
Lymph node metastasis	Negative	53	4	49	0.0111	4	48	0.0051
	Positive	32	9	23		10	22
Distant metastasis	Negative	71	8	63	0.0201	6	65	0.0001
	Positive	14	5	9		8	5
Dukes stage	A	15	0	15		0	15
	B	33	2	31	0.0082	2	31	0.0002
	C	23	6	17		4	19
	D	14	5	9		8	5

¹Chi-square test;

²Fisher’s exact test.